A5037687427- Autophagy in Disease and Therapy
- Sirtuins and Resveratrol in Medicine
- Pluripotent Stem Cells Research
- Cancer, Hypoxia, and Metabolism
- Calcium signaling and nucleotide metabolism
- Renal Diseases and Glomerulopathies
- Genetic and Kidney Cyst Diseases
- Congenital heart defects research
- Hippo pathway signaling and YAP/TAZ
- Error Correcting Code Techniques
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Developmental Biology and Gene Regulation
- Dialysis and Renal Disease Management
- Coronary Artery Anomalies
- Multilevel Inverters and Converters
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Erythropoietin and Anemia Treatment
- Chronic Kidney Disease and Diabetes
- Advanced Wireless Communication Techniques
- Congenital Heart Disease Studies
- Pancreatic function and diabetes
- Tuberous Sclerosis Complex Research
- Acute Kidney Injury Research
Second Affiliated Hospital of Nanjing Medical University
2019-2024
National Institute of Environmental Health Sciences
2015-2023
University of Chinese Academy of Sciences
2017-2023
Center for Excellence in Molecular Cell Science
2022-2023
Triangle
2021
Nanjing Medical University
2014-2020
Shanghai Tenth People's Hospital
2020
Tongji University
2020
Guangdong University of Technology
2016-2018
Southeast University
2018
Recent lineage-tracing studies have produced conflicting results about whether the epicardium is a source of cardiac muscle cells during heart development. Here, we examined developmental potential epicardial tissue in zebrafish both embryonic development and injury-induced regeneration. We found that upstream sequences transcription factor gene tcf21 activated robust, epicardium-specific expression throughout Cre recombinase-based, genetic fate-mapping larval or adult tcf21+ revealed...
Certain lower vertebrates like zebrafish activate proliferation of spared cardiomyocytes after cardiac injury to regenerate lost heart muscle. Here, we used translating ribosome affinity purification profile RNAs in during regeneration. We identified dynamic induction several Jak1/Stat3 pathway members following trauma, events accompanied by cytokine production. Transgenic Stat3 inhibition restricted injury-induced and regeneration, but did not reduce cardiogenesis animal growth. The...
Inhibition of sodium-glucose cotransporter 2 (SGLT2) in the proximal tubule kidney has emerged as an effective antihyperglycemic treatment. The potential protective role SGLT2 inhibition on diabetic disease (DKD) and underlying mechanism, however, remains unknown. In this study, metabolic switch was examined using samples from human with diabetes streptozocin (STZ)-induced experimental mouse model treated or without inhibitor dapagliflozin. Results were further validated primarily cultured...
Highlights•Transgenic skinbow zebrafish barcodes skin superficial epithelial cells (SECs)•Hundreds of SECs are simultaneously monitored in vivo over cell surface lifetimes•SEC behaviors quantified during regeneration wounds or amputation injuries•Identification and quantification ROS effects on SEC regenerationSummaryCurrent fate mapping imaging platforms limited their ability to capture dynamic cells. To deconstruct regenerating adult tissue at single-cell resolution, we created a...
Methionine metabolism is critical for epigenetic maintenance, redox homeostasis, and animal development. However, the regulation of methionine remains unclear. Here, we provide evidence that SIRT1, most conserved mammalian NAD+-dependent protein deacetylase, critically involved in modulating metabolism, thereby impacting maintenance mouse embryonic stem cells (mESCs) subsequent embryogenesis. We demonstrate SIRT1-deficient mESCs are hypersensitive to restriction/depletion-induced...
Abstract Podocytes, a type of highly specialized epithelial cells, require substantial levels energy to maintain glomerular integrity and function, but little is known on the regulation podocytes’ energetics. Lack metabolic analysis during podocyte development led us explore distribution mitochondrial oxidative phosphorylation glycolysis, two major pathways cell metabolism, in cultured podocytes vitro differentiation. Unexpectedly, we observed stronger glycolytic profile, accompanied by an...
Caudal fin regeneration is characterized by a proliferation boost in the mesenchymal blastema controlled precisely time and space. This allows gradual robust restoration of original size. However, how this established regulated not well understood. Here we report that Yap, Hippo pathway effector, chief player process: functionally manipulating Yap during dramatically affects cell expression key signaling pathways, impacting regenerative growth. The intracellular location tightly associated...
Mitochondrial dysfunction leads to loss of renal function and structure; however, the precise mechanisms by which mitochondrial can regulate fibrosis remain unclear. Proximal tubular cells (PTCs) prefer fatty acid oxidation as their energy source dysregulation lipid metabolism has been linked tubulointerstitial (TIF). Here, we demonstrated that uncoupling protein 2 (UCP2) regulates TIF through stimulation deposition extracellular matrix (ECM) accumulation. We show UCP2 expression was...
Sphingolipids are important structural components of cell membranes and prominent signaling molecules controlling growth, differentiation, apoptosis. particularly abundant in the brain, defects sphingolipid degradation associated with several human neurodegenerative diseases. However, molecular mechanisms governing metabolism remain unclear. Here, we report that is under transcriptional control SIRT1, a highly conserved mammalian NAD+-dependent protein deacetylase, mouse embryonic stem cells...
Chromosomes occupy discrete spaces in the interphase cell nucleus, called chromosome territory. The structural and functional relevance of territory remains elusive. We fused 15 17 mouse haploid embryonic stem cells (haESCs), resulting distinct changes territories cognate chromosomes, but with little effect on gene expression, pluripotency gamete functions haESCs. karyotype-engineered haESCs were successfully implemented generating heterozygous (2n = 39) homozygous 38) models. Mice...
Acute kidney injury (AKI) is a devastating condition with high morbidity and mortality rates. The pathological features of AKI are tubular injury, infiltration inflammatory cells, impaired vascular integrity. Pyruvate kinase the final rate-limiting enzyme in glycolysis pathway. We previously showed that pyruvate M2 (PKM2) plays an important role regulating glycolytic reprogramming fibroblasts renal interstitial fibrosis. present study aimed to determine PKM2 fibroblast activation during...
Podocytes play a key role in the formation of cellular crescents experimental and human diseases. However, underlying mechanisms for podocytes promoting crescent need further investigation. Here, we demonstrated that mammalian target rapamycin complex 1 (mTORC1) signaling was remarkably activated hypoxia-inducible factor (HIF) 1α expression largely induced from patients with crescentic glomerular Specific deletion Tsc1 led to mTORC1 activation kidney dysfunction mice. Interestingly, 33 36...
An iterative detection algorithm, based on fixed length sliding window, is proposed for multi-function phased array radar (MPAR) behavior recognition. First, characteristic parameters such as frequency, pulse width, amplitude, repetition interval and beam orientation were extracted. Then the sequence divided by appropriate fixed-length window. Finally, conditional probability calculated step Bayesian criterion, result compared with previous to determine whether it a change point. This...
Training and experiences are usually required to successfully culture differentiate human embryonic stem cells (hESCs). Here, we describe a simple but highly efficient protocol induce endoderm differentiation of hESCs with crotonate, precursor crotonyl-CoA for histone crotonylation deposition on endodermal genes. In this protocol, adding crotonate in different media significantly increases the efficiency substantially reduces amount reagents. For complete details use execution please refer...