A5031636414- Receptor Mechanisms and Signaling
- Analytical Chemistry and Chromatography
- Monoclonal and Polyclonal Antibodies Research
- Traditional Chinese Medicine Analysis
- Pharmacological Effects and Assays
- Protein Interaction Studies and Fluorescence Analysis
- Advanced Biosensing Techniques and Applications
- Chemical Synthesis and Analysis
- Computational Drug Discovery Methods
- Neuropeptides and Animal Physiology
- Ginseng Biological Effects and Applications
- Metabolomics and Mass Spectrometry Studies
- Pharmacological Effects of Natural Compounds
- Plant-based Medicinal Research
- Neuroscience and Neuropharmacology Research
- Advanced biosensing and bioanalysis techniques
- Protein purification and stability
- Click Chemistry and Applications
- Traditional Chinese Medicine Studies
- Biochemical and Structural Characterization
- Medicinal Plants and Neuroprotection
- Molecular spectroscopy and chirality
- Drug Solubulity and Delivery Systems
- Electrochemical Analysis and Applications
- Berberine and alkaloids research
Ministry of Education of the People's Republic of China
2015-2025
Northwest University
2016-2025
Yellow River Conservancy Technical Institute
2023-2025
Guangdong Province Environmental Monitoring Center
2024
North China University of Science and Technology
2023-2024
Hangzhou Children's Hospital
2023
Northwest University
2008-2022
Capital Normal University
2005-2021
Purdue University West Lafayette
2017
China XD Group (China)
2015
Microglia-mediated neuroinflammation plays a crucial role in the pathophysiological process of multiple neurological disorders such as ischemic stroke, yet lacks effective therapeutic agents. Previously, we discovered one novel synthetic compound, tanshinol borneol ester (DBZ), possesses anti-inflammatory and anti-atherosclerotic activities, whereas little is known about its effects CNS. Therefore, present study aims to explore potential mechanism DBZ on microglial function. Our studies...
Objective Omeprazole is metabolized by genetically determined S-mephenytoin 4′-hydroxylase (CYP2C19) in the liver. This study aimed to determine whether effect of omeprazole on intragastric pH depends CYP2C19 genotype status. Methods status for 2 mutations associated with poor metabolizer phenotype was a polymerase chain reaction–restriction fragment length polymorphism method 16 healthy volunteers. Helicobacter pylori serology and [13C]urea breath test. After single oral administration 20...
Significance Obesity is associated with enhanced colonic inflammation, which a major risk factor for colorectal cancer. To date, the mechanisms by obesity increases inflammation are not well-understood, and there few effective strategies controlling obesity-induced diseases. Here, using LC-MS/MS–based metabolomics, we report that soluble epoxide hydrolase (sEH) could be novel therapeutic target inflammation. This lead to rapid human translation, as pharmacological inhibitors of sEH being...
An approach is established for the specific immobilization of GPCRs from cell lysates that circumvents labor intensive purification procedures and minimize loss activity.
Purposes: To investigate whether valproic acid (VPA) has a neuroprotective effect against ischemia/reperfusion (I/R) injury in the rat retina, and to elucidate potential antioxidant mechanisms involved.Methods: Adult male Wistar rats were randomly divided into four groups: sham (group A), plus VPA B), I/R vehicle C), D). Retinal was produced by inducing an exceedingly high intraocular pressure (IOP). Prior insult, administered subcutaneously (300 mg/kg twice daily) for 7 days, after which...
Protein immobilization is particularly significant in proteomics, interactomics, and vitro drug screening. It an essential primary step for numerous biological techniques that rely on immobilized proteins with controlled orientation, high conformational stability, activity (CHH). These have challenged the current strategy demanded increasing efforts efficient method to meet CHH a single step. Herein, we proposed covalent inhibitor-based, one-step G protein-coupled receptor (GPCR) inspired by...
The past decade has witnessed the great promise of strategies for ligand discovery based on surface-immobilized GPCRs. We present here a method preparation immobilized Key features include covalent immobilization with high specificity and robust application in drug-receptor interaction analysis screening. In our example assay using beta2-adrenergic receptor (β2-AR), human DNA repair protein O6-alkylguanine-DNA alkyltransferase (hAGT) fusion expressed Escherichia coli was directly captured...
Responsive self-assembly is a general process in biological systems and highly desired engineered systems. DNA nanostructures provide versatile molecular platform for studying such responsive self-assembly. Various triggers have been explored nanostructures. However, each trigger requires unique mechanism its response. This situation brings great challenge to engineer the responsiveness. Herein, we propose an aptamer-based, allosteric The aptamer–ligand binding causes motif change...
Although a comparatively robust method, immobilized protein-based techniques have displayed limited precision and inconsistent results due to lack of strategy for the accurate selection drug adsorption models on protein surface. We generated data three drugs beta-2-adrenoceptor (β2-AR) by frontal affinity chromatography–mass spectrometry (FAC-MS) site-specific competitive FAC-MS. Using energy distribution (AED) calculations, we achieved best binding salbutamol, terbutaline, pseudoephedrine...
Natural products have failed to meet the urgent need for drug discovery in recent decades due limited resources, necessitating new strategies re-establishing key role of natural hit screening. This work introduced DNA-encoding techniques into synthesis phenolic acid-focused libraries containing 32 000 diverse compounds. Online selection library using immobilized angiotensin II type I receptor (AT
Allosteric ligands are promising drugs owing to their remote regulations of the orthosteric ligand signaling pathway. There few allosteric due lack handy and efficacious method for screening. Herein, we developed an affinity chromatographic screening by immobilizing purified beta2 adrenoceptor (β2-AR) onto macroporous silica gel a two-point tethering method. The relies on occupation site antagonist chelation N-terminal His-tag receptor Ni2+ coated gel. immobilized β2-AR demonstrated greatest...
α-Asaronol from Acorus tatarinowii (known as "Shichangpu" in Traditional Chinese medicine) has been proved to possess more efficient antiepileptic activity and lower toxicity than α-asarone (namely "Xixinnaojiaonang" an drug China) our previous study. However, the molecular mechanism of α-asaronol against epilepsy needs be known if become a novel medicine. Nuclear magnetic resonance (NMR)-based metabolomics was applied investigate metabolic patterns plasma brain tissue extract...
Abstract Background Cutaneous hypertrophic scar is a fibro-proliferative hard-curing disease. Recent studies have proved that antagonists of angiotensin II type 1 receptor (AT R) and agonists 2 were able to relieve scar. Therefore, establishing new methods pursue dual-target lead compounds from Chinese herbs in much demand for treating Methods To this end, we immobilized AT R or onto the surface silica gel cell lysates through specific covalent bond by bioorthogonal chemistry. The columns...