A5084442661- Neuroendocrine regulation and behavior
- Stress Responses and Cortisol
- Parkinson's Disease Mechanisms and Treatments
- MicroRNA in disease regulation
- Neurogenesis and neuroplasticity mechanisms
- Genetics and Neurodevelopmental Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Nerve injury and regeneration
- Autism Spectrum Disorder Research
- Nuclear Receptors and Signaling
- Neuroscience and Neuropharmacology Research
- Birth, Development, and Health
- Computational Drug Discovery Methods
- Circular RNAs in diseases
- Neurotransmitter Receptor Influence on Behavior
- Early Childhood Education and Development
- Transcranial Magnetic Stimulation Studies
- Machine Learning in Healthcare
- Neurological disorders and treatments
- Receptor Mechanisms and Signaling
- Spectroscopy Techniques in Biomedical and Chemical Research
- Resilience and Mental Health
- Alzheimer's disease research and treatments
- Adipose Tissue and Metabolism
- Cancer-related molecular mechanisms research
Fondazione Santa Lucia
2020-2024
Institute of Cognitive Sciences and Technologies
2024
Research Network (United States)
2024
Istituti di Ricovero e Cura a Carattere Scientifico
2020-2023
Sapienza University of Rome
2019-2022
Over the last few decades, emerging evidence suggests that non-coding RNAs (ncRNAs) including long-non-coding RNA (lncRNA), microRNA (miRNA) and circular-RNA (circRNA) contribute to molecular events underlying progressive neuronal degeneration, a plethora of ncRNAs have been identified significantly misregulated in many neurodegenerative diseases, Parkinson’s disease synucleinopathy. Although direct link between neuropathology causative candidates has not clearly established cases,...
Abstract Background The accumulation of α‐synuclein (α‐syn) fibrils in intraneuronal inclusions called Lewy bodies and neurites is a pathological signature Parkinson's disease (PD). Although several aspects linked to α‐syn–dependent pathology (concerning its spreading, aggregation, activation inflammatory neurodegenerative processes) have been under intense investigation, less attention has devoted the real impact α‐syn overexpression on structural functional properties substantia nigra pars...
Abstract Increasing efforts have been made to elucidate how genetic and environmental factors interact in Parkinson’s disease (PD). In the present study, we assessed development of symptoms on a PD rat model that overexpresses human α-synuclein ( Snca +/+ ) at presymptomatic age, exposed pro-inflammatory insult by intraperitoneal injection lipopolysaccharide (LPS), using immunohistology, high-dimensional flow cytometry, constant potential amperometry, behavioral analyses. A single LPS into...
Protocol for quantifying TH+ fibers in rat dorsal striatum and nucleus accumbens core shell
Protocol for cell counting using StereoInvestigator software.
Protocol for cell counting using StereoInvestigator software.
Neuromelanin (NM) is a dark pigment accumulating with age in human substantia nigra pars compacta (SNpc) dopamine (DA) neurons, conferring the look that inspired nigral area's name. Despite NM has long been associated Parkinson's disease (PD), as melanized neurons favorably degenerate during development, functions within SNpc DA are still mostly elusive. Here, by exploiting an NM-producing rat model generated viral vector-induced expression of Tyrosinase (hTyr), we inspected impact on...
Exposure to aversive events during sensitive developmental periods can affect the preferential coping strategy adopted by individuals later in life, leading either stress-related psychiatric disorders, including depression, or well-adaptation future adversity and sources of stress, a behavior phenotype termed "resilience". We have previously shown that interfering with development mother-pups bond Repeated Cross Fostering (RCF) stress protocol induce resilience depression-like adult C57BL/6J...
Abstract Female, but not male, mice with haploinsufficiency for the proautophagic Ambra1 gene show an autistic-like phenotype associated hippocampal circuits dysfunctions which include loss of parvalbuminergic interneurons (PV-IN), decrease in inhibition/excitation ratio, and abundance immature dendritic spines on CA1 pyramidal neurons. Given paucity data relating to female autism, we exploit +/− model investigate whether rectifying inhibitory input onto principal neurons (PN) rescues their...
Early life experiences that affect the attachment bond formation can alter developmental trajectories and result in pathological outcomes a sex-related manner. However, molecular basis of sex differences is quite unknown. The dopaminergic system originating from ventral tegmental area has been proposed to be key mediator this process. Here we exploited murine model early adversity (Repeated Cross Fostering, RCF) test how interfering with affects VTA-related functions sex-specific Through...
Repetitive stereotyped behaviors are core symptoms of autism spectrum disorders (ASD) and fragile X syndrome (FXS), the prevalent genetic cause intellectual disability autism. The nigrostriatal dopamine (DA) circuit rules movement creation habits sequential behaviors; therefore, its dysregulation could promote autistic repetitive behaviors. Nevertheless, inspection substantia nigra pars compacta (SNpc) DA neurons in ASD models has been overlooked specific evidence their altered activity FXS...
Alzheimer's disease (AD) diagnosis often requires invasive examinations (e.g., liquor analyses), expensive tools brain imaging) and highly specialized personnel. The commonly is established when the disorder has already caused severe damage, clinical signs begin to be apparent. Instead, accessible low-cost approaches for early identification of subjects at high risk developing AD years before they show overt symptoms are fundamental provide a critical time window more effective management,...
The cellular mechanisms regulating dopamine (DA) release in the striatum have attracted much interest recent years. By vitro amperometric recordings mouse striatal slices, we show that a brief (5 min) exposure to metabotropic glutamate receptor agonist DHPG (50 μM) induces profound depression of synaptic DA release, lasting over 1 h from washout. This long-term is sensitive glycine, which preferentially inhibits local cholinergic interneurons, as well drugs acting on nicotinic acetylcholine...
Helplessness is a dysfunctional coping response to stressors associated with different psychiatric conditions. The present study tested the hypothesis that early and adult adversities cumulate produce helplessness depending on genotype (3-hit of psychopathology). To this aim, we evaluated whether Chronic Unpredictable Stress (CUS) differently affected mesoaccumbens dopamine (DA) responses stress challenge by mice C57BL/6J (B6) DBA/2J (D2) inbred strains life experience (Repeated Cross...
ABSTRACT Increasing efforts have been made to elucidate how genetic and environmental factors interact in Parkinson’s disease (PD). In the present study, we assessed development of symptoms on a PD rat model that overexpresses human α-synuclein ( Snca +/+ ) at presymptomatic age, exposed pro-inflammatory insult by intraperitoneal injection lipopolysaccharide (LPS), using immunohistology, high-dimensional flow cytometry, constant potential amperometry, behavioral analyses. A single LPS into...
Abstract Repetitive behaviors are core symptoms of autism spectrum disorders (ASD) and fragile X syndrome (FXS), the prevalent genetic cause intellectual disability autism. The nigrostriatal dopamine (DA) circuit rules movement habit formation; therefore, its dysregulation stands as a leading substrate for repetitive behaviors. However, beyond indirect evidence, specific assessment nigral DA neuron activity in ASD FXS models is lacking. Here, we show that hyperactivity substantia nigra pars...