A5077567483- Optical Coherence Tomography Applications
- Photoacoustic and Ultrasonic Imaging
- Advanced Fluorescence Microscopy Techniques
- Optical Imaging and Spectroscopy Techniques
- Renal and Vascular Pathologies
- Anesthesia and Pain Management
- Spectroscopy Techniques in Biomedical and Chemical Research
- Retinal Imaging and Analysis
- Photodynamic Therapy Research Studies
- Neural dynamics and brain function
- Nanoplatforms for cancer theranostics
- Intraoperative Neuromonitoring and Anesthetic Effects
- Intraocular Surgery and Lenses
- Angiogenesis and VEGF in Cancer
- Spine and Intervertebral Disc Pathology
- Gastrointestinal Bleeding Diagnosis and Treatment
- Photoreceptor and optogenetics research
- Kidney Stones and Urolithiasis Treatments
- Retinal and Macular Surgery
- Neuroscience and Neural Engineering
- Neurosurgical Procedures and Complications
- Corneal surgery and disorders
- Cardiac, Anesthesia and Surgical Outcomes
- Advanced X-ray and CT Imaging
- Elasticity and Material Modeling
University of Oklahoma
2019-2024
University of Oklahoma Health Sciences Center
2021-2024
Worcester Polytechnic Institute
2023
University of Maryland, College Park
2014-2021
University of Maryland, Baltimore
2018
Children's National
2015
Abstract Chemotherapy‐induced cognitive impairment (“chemobrain”) is a frequent side‐effect in cancer survivors treated with paclitaxel (PTX). The mechanisms responsible for PTX‐induced remain obscure, and there are no effective treatments or prevention strategies. Here, we test the hypothesis that PTX induces endothelial senescence, which impairs microvascular function contributes to genesis of decline. We transgenic p16‐3MR mice, allows detection selective elimination senescent cells, (5...
Kidney ischemia-reperfusion (I/R) accounts for the majority of acute kidney injury cases, whose consequences are commonly encountered after transplantation. Optical coherence tomography (OCT) has been applied to image changes in microanatomy and microcirculation. In this paper, we demonstrate a quantitative method monitoring status during I/R process using texture properties OCT images. This approach employs skewness measure distribution en face intensities at different depths, thus allowing...
We report the development of a quantum dot (QD)–peptide–fullerene (C60) electron transfer (ET)-based nanobioconjugate for visualization membrane potential in living cells. The bioconjugate is composed (1) central QD donor, (2) membrane-inserting peptidyl linker, and (3) C60 acceptor. photoexcited donor engages ET with acceptor, resulting quenching photoluminescence (PL) that tracks positively number moieties arrayed around QD. nature QD-capping ligand also modulates efficiency; neutral...
Age-related blood-brain barrier (BBB) disruption and cerebromicrovascular rarefaction contribute importantly to the pathogenesis of both vascular cognitive impairment dementia (VCID) Alzheimer's disease (AD). Recent advances in geroscience research enable development novel interventions reverse age-related alterations cerebral microcirculation for prevention VCID AD. To facilitate this research, there is an urgent need sensitive easy-to-adapt imaging methods that longitudinal assessment...
Abstract Optical coherence tomography (OCT) can be used to image microstructures of human kidneys. However, current OCT probes exhibit inadequate field-of-view, leading potentially biased kidney assessment. Here we present a robotic system where the probe is integrated robot manipulator, enabling wider area (covers an 106.39 mm by 37.70 mm) spatially-resolved imaging. Our comprehensively scans surface at optimal altitude with preoperative path planning and image-based feedback control...
Early detection of neoplastic changes remains a critical challenge in clinical cancer diagnosis and treatment. Many cancers arise from epithelial layers such as those the gastrointestinal (GI) tract. Current standard endoscopic technology is difficult to detect subsurface lesions. In this research, we investigated feasibility novel multi-modal optical imaging approach including high-resolution coherence tomography (OCT) high-sensitivity fluorescence laminar (FLOT) for structural molecular...
Emerging three-dimensional (3D) printing technology enables the fabrication of optically realistic and morphologically complex tissue-simulating phantoms for development evaluation novel optical imaging products. In this study, we assess potential to print image-defined neurovascular with patent channels contrast-enhanced near-infrared fluorescence (NIRF) imaging. An anatomical map defined from clinical magnetic resonance (MRI) was segmented processed into files suitable a forebrain vessel...
We present a simple but effective method to quantitatively measure the birefringence of tissue by an all single-mode fiber (SMF) based polarization-sensitive optical coherence tomography (PS-OCT) with single input polarization state. theoretically verify that our SMF PS-OCT system can quantify phase retardance and optic axis orientation after calibration process using quarter wave plate (QWP). Based on proposed method, quantification Berek compensator biological tissues were demonstrated.
Preoperative neoadjuvant treatment in locally advanced breast cancer is recognized as an effective adjuvant therapy, it improves outcomes.However, the potential complications remain a threat, so there urgent clinical need to assess both tumor response and changes its microenvironment using non-invasive precise identification techniques.Here, two-photon microscopy was employed detect morphological alterations progression recession throughout chemotherapy.The structure were analyzed based on...
Abstract The imaging of real‐time fluxes K + ions in live cell with high dynamic range (5–150 × 10 −3 m ) is paramount importance for neuroscience and physiology the gastrointestinal tract, kidney, other tissues. In particular, research on high‐performance deep‐red fluorescent nanoparticle‐based biosensors highly anticipated. It found that boron‐dipyrromethene (BODIPY)‐based ‐sensitive fluoroionophore FI3 encapsulated cationic polymer RL100 nanoparticles displays unusually strong efficiency...
Intracranial aneurysms (ICAs) are focal dilatations that imply a weakening of the brain artery. Incidental rupture an ICA is increasingly responsible for significant mortality and morbidity in American's aging population. Previous studies have quantified pressure-volume characteristics, uniaxial mechanical properties, morphological features human aneurysms. In this pilot study, first time, we comprehensively mechanical, collagen fiber microstructural, properties one resected posterior...
Abstract Cerebral microvascular health is a key biomarker for the study of natural aging and associated neurological diseases. Our aim to quantify aging‐associated change microvasculature at diverse dimensions in mice brain. We used optical coherence tomography (OCT) two‐photon microscopy (TPM) obtain nonaged aged C57BL/6J cerebral images vivo. results indicated that artery & vein, arteriole venule, capillary from showed significant differences density, diameter, complexity, perimeter,...
Human liver transplantation is severely constrained by a critical shortage of donor livers, with approximately one quarter patients on the waiting list dying due to scarcity viable organs. Current viability assessments, which rely invasive pathological methods, are hampered limited sampling from biopsies, particularly in marginal livers extended criteria donors (ECD) intended expand pool. Consequently, there pressing need for more comprehensive and non-invasive evaluation techniques meet...
Abstract The whisker system of rodents is an excellent model to study peripherally evoked neural activity in the brain. Discrete modules represent each somatosensory cortex (“barrels”), thalamus (“barreloids”) and brain stem (“barrelettes”). Stimulation a single evokes sequentially its corresponding barrelette, barreloid barrel. Conventional optical imaging functional activation limited surface structures such as cerebral cortex. To access subcortical image sensory-evoked activity, we...
Epidural anesthesia is one of the most widely used methods. We developed a small hand-held OCT forward-imaging needle device for real-time epidural surgery guidance and demonstrated its feasibility through ex vivo experiments.
Epidural anesthesia is one of the most widely used methods. Due to lack visual feedback guide needle navigation, failure rate epidural up 20%, and complication peripheral nerve block approaches 10%, with potential permanent damage. To address these difficulties, insertion under ultrasound guidance fluoroscopy has been introduced. However, they do not provide adequate resolution contrast distinguish tissue layers that travels through or specifically identify space. improve accuracy space...
Three-dimensional fluorescence laminar optical tomography (FLOT) can achieve resolutions of 100-200 µm and penetration depths 2-3 mm. FLOT has been used in tissue engineering, neuroscience, as well oncology. The limited dynamic range the charge-coupled device-based system makes it difficult to image fluorescent samples with a large concentration difference, limits its depth, diminishes quantitative accuracy 3D reconstruction data. Here, incorporating high-dynamic-range (HDR) method widely...
Abstract Functional mapping of brain activity is important in elucidating how neural networks operate the living brain. The whisker sensory system rodents an excellent model to study peripherally evoked central nervous system. Each facial represented by discrete modules neurons all along pathway leading neocortex. These are called “barrels” layer 4 primary somatosensory cortex. Their location (approximately 300–500 μm below cortical surface) allows for convenient imaging whisker-evoked vivo...
The three-dimensional (3D) tumor spheroid model is a critical tool for high-throughput ovarian cancer research and anticancer drug development