A5062643699- HIV Research and Treatment
- Pneumocystis jirovecii pneumonia detection and treatment
- HIV/AIDS drug development and treatment
- Pneumonia and Respiratory Infections
- HIV/AIDS Research and Interventions
- Respiratory viral infections research
- Immune Cell Function and Interaction
- HIV-related health complications and treatments
- COVID-19 Clinical Research Studies
- Hepatitis C virus research
- Colorectal and Anal Carcinomas
- Cervical Cancer and HPV Research
- Antibiotics Pharmacokinetics and Efficacy
- Tuberculosis Research and Epidemiology
- SARS-CoV-2 and COVID-19 Research
- Viral-associated cancers and disorders
- Antibiotic Resistance in Bacteria
- Cytomegalovirus and herpesvirus research
- Infectious Diseases and Tuberculosis
- T-cell and B-cell Immunology
- Hepatitis B Virus Studies
- Liver Disease Diagnosis and Treatment
- Bacterial Infections and Vaccines
- Research on Leishmaniasis Studies
- COVID-19 Impact on Reproduction
Universitat Autònoma de Barcelona
2016-2025
Vall d'Hebron Institut de Recerca
2016-2025
Vall d'Hebron Hospital Universitari
2013-2025
Botkin Hospital
2021
Hebron University
2009-2018
Office of Infectious Diseases
2008-2017
Instituto Maimónides de Investigación Biomédica de Córdoba
2017
Hospital Universitario Reina Sofía
2017
Universitat de Barcelona
2005-2013
Weatherford College
2011
Latency reversal agents (LRAs) have proven to induce HIV-1 transcription in vivo but are ineffective at decreasing the size of latent reservoir antiretroviral treated patients. The capacity LRAs perturb viral present distinct subpopulations cells is currently unknown. Here, using a new RNA FISH/flow ex reactivation assay, we performed comprehensive assessment different families LRAs, and their combinations, CD4+ T cell subsets. We observed that median 16.28% whole HIV-reservoir induced...
Abstract Resident memory T cells (T RM ) positioned within the respiratory tract are probably required to limit SARS-CoV-2 spread and COVID-19. Importantly, mostly non-recirculating, which reduces window of opportunity examine these in blood as they move lung parenchyma. Here, we identify circulating virus-specific cell responses during acute infection with functional, migratory apoptotic patterns modulated by viral proteins associated clinical outcome. Disease severity is predominantly IFNγ...
Ceftaroline, the active form of prodrug ceftaroline fosamil, is a novel cephalosporin with bactericidal activity against important pathogens associated community-acquired pneumonia (CAP), including Streptococcus pneumoniae and common Gram-negative pathogens. FOCUS 1 randomized, double-blinded, Phase III study that was conducted to evaluate efficacy safety fosamil in treating patients CAP. The primary objective determine non-inferiority [lower limit 95% confidence interval (CI) ≥ −10%]...
Lactic acidosis is a rare but often fatal complication reported in some human immunodeficiency virus (HIV)-infected patients treated with nucleoside-analogue reverse-transcriptase inhibitors. We report series of 12 HIV infection nucleoside analogues who developed unexplained metabolic acidosis. have also reviewed 60 additional published cases. The aim the present study to describe clinical picture, prognostic factors, and final outcome for nucleoside-associated lactic mortality rate high:...
Cells that actively transcribe HIV-1 have been defined as the "active viral reservoir" in HIV-infected individuals. However, important technical limitations precluded characterization of this specific reservoir during both treated and untreated infections. Here, we used a novel single-cell RNA fluorescence situ hybridization-flow cytometry (FISH-flow) assay requires only 15 million unfractionated peripheral blood mononuclear cells (PBMCs) to characterize cell subpopulations HIV different...
Abstract HIV viral reservoirs are established very early during infection. Resident memory T cells (T RM ) present in tissues such as the lower female genital tract, but contribution of this subset to pathogenesis and persistence remains unclear. Here, we show that cervical CD4 + display a unique repertoire clusters differentiation, with enrichment several molecules associated infection susceptibility, longevity self-renewing capacities. These protein profiles enriched fraction expressing...
Our objective was to assess the therapeutic noninferiority of dual therapy with darunavir/ritonavir and lamivudine compared triple plus 2 nucleos(t)ides for maintenance human immunodeficiency virus type 1 (HIV-1) suppression.This a multicenter, open-label, trial (margin 12%). Patients HIV-1 RNA <50 copies/mL 6 months or longer on (tenofovir disoproxil fumarate emtricitabine abacavir lamivudine) no resistance were randomized continue (n = 128) switch 129). The primary endpoint proportion...
BackgroundFactors affecting outcomes of SARS-CoV-2 infection in people living with HIV are unclear. We assessed the factors associated diagnosis and severe among HIV.MethodsWe did a retrospective cohort study using data from PISCIS Catalonia (Spain) between March 1 Dec 15, 2020. linked integrated health-care, clinical, surveillance registries through Public Data Analysis for Health Research Innovation Program (PADRIS) to obtain on diagnosis, chronic comorbidities, as well clinical mortality...
We studied all human immunodeficiency virus (HIV)-infected patients with invasive pneumococcal disease who received their diagnosis during 1996-2002 to investigate the incidence of this in highly active antiretroviral therapy era and study influence CD4 lymphocyte count on clinical presentation outcome disease. The overall was 11.3 cases per 100,000 person-years adult without known HIV infection 677 HIV-infected patients. This remained stable over period. Clinical presentation, severity...
To determine whether rifampicin reduces serum concentrations of nevirapine and modifies rifampicin, levels these agents were determined at steady state by high-performance liquid chromatography in 10 HIV-infected patients with tuberculosis. The median area under the curve (AUC) 0-12h before after was 56.2 32.8 microg/ml per hour, respectively ( p =.04). This represents a 31% reduction concentrations. C(max) decreased from 5.6 to 4.5 =.04), which represented 36% reduction. A 21% decrease...
The role of pneumococcal urinary antigen detection in the treatment adults with community-acquired pneumonia (CAP) is not well defined. We assessed usefulness diagnosis and antimicrobial guidance patients hospitalized CAP.A prospective study all CAP was performed from February 2007 through January 2008. To evaluate accuracy test, we calculated its sensitivity, specificity, positive negative predictive values, likelihood ratios. gold standard used for isolation blood or pleural fluid...
Human immunodeficiency virus (HIV) infection induces immunological dysfunction, which limits the elimination of HIV-infected cells during treated infection. Identifying and targeting dysfunctional immune might help accelerate purging persistent viral reservoir. Here, we show that chronic HIV increases natural killer (NK) cell populations expressing negative regulator KLRG1, both in peripheral blood lymph nodes. Antiretroviral treatment (ART) does not reestablish these functionally impaired...
Abstract Resident memory T cells (T RM ) present at the respiratory tract may be essential to enhance early SARS-CoV-2 viral clearance, thus limiting infection and disease. While long-term antigen-specific are detectable beyond 11 months in lung of convalescent COVID-19 patients, it is unknown if mRNA vaccination encoding for S-protein can induce this frontline protection. Here we show that frequency CD4 + secreting IFNγ response S-peptides variable but overall similar mRNA-vaccinated...
Background: The aim of this study was to analyze the incidence new cases, survival HIV-1-infected patients with progressive multifocal leukoencephalopathy (PML), and characteristics PML-associated immune reconstitution inflammatory syndrome (IRIS). Methods: Multicenter observational cohort all newly diagnosed PML in 7 hospitals Barcelona (Spain) from 2002 2006. annual calculated. Survival estimated using Kaplan-Meier method. IRIS defined as onset or rapid worsening shortly after initiation...
Background. Increased rates of empyema have been reported in children after the introduction pneumococcal conjugate vaccine (PCV7). Our objective was to describe risk factors for adults and analyze differences incidence, disease characteristics, serotype distribution between pre- post-PCV7 eras.
Abstract The identification of exclusive markers to target HIV-reservoir cells will represent a significant advance in the search for therapies cure HIV. Here, we identify B lymphocyte antigen CD20 as marker HIV-infected vitro and vivo. molecule is dimly expressed subpopulation CD4-positive (CD4 + ) T lymphocytes from blood, with high levels cell activation heterogeneous memory phenotypes. In lymph node samples infected patients, present productively cells, ex vivo viral infection...
The development of physiological models that reproduce SARS-CoV-2 infection in primary human cells will be instrumental to identify host-pathogen interactions and potential therapeutics. Here, using cell suspensions directly from lung tissues (HLT), we have developed a rapid platform for the identification viral targets expression entry factors, as well screening inhibitors anti-inflammatory compounds. direct use HLT cells, without long-term culture vitro differentiation approaches,...
Vaccines against smallpox are known to have cross-protective activity monkeypox, and monkeypox infections believed generate permanent immunity. Nevertheless, there scarce data about the possibility of reinfection or reactivation. Recently, a case apparent has been reported. We present suspected second episode in healthy previously vaccinated man, with confirmed primary infection occurring three months before presentation.
Background: Lipoatrophy is the most stigmatizing side effect of stavudine therapy. We assessed long-term effects replacing with tenofovir in HIV-infected patients lipoatrophy. Method: Prospective switch study. Sixty-two clinically stable antiretroviral therapy (ART) containing stavudine, HIV-1 RNA <50 copies/mL, and lipoatrophy at least face on physical examination were included. All switched from to without changing any other drug. Objective (malar ultasonography, bioelectrical impedance...
Latent parasitic infections can reactivate because of immunosuppression. We conducted a prospective observational study all human immunodeficiency virus (HIV)–infected immigrants who visited the Infectious Diseases Department Hospital Universitari Vall d'Hebron, Barcelona, Spain, during June 2010–May 2011. Screening most prevalent tropical diseases (intestinal parasitosis, Chagas disease, leishmaniasis, malaria, schistosomiasis, and strongyloidiasis) was performed according to geographic...