A5044467196- Platelet Disorders and Treatments
- Iron Metabolism and Disorders
- Hemoglobinopathies and Related Disorders
- Blood groups and transfusion
- Antibiotics Pharmacokinetics and Efficacy
- Blood properties and coagulation
- Renal function and acid-base balance
- Advancements in Transdermal Drug Delivery
- Clinical Laboratory Practices and Quality Control
- Nanoparticle-Based Drug Delivery
- Blood Coagulation and Thrombosis Mechanisms
- 3D Printing in Biomedical Research
- Blood disorders and treatments
- Erythropoietin and Anemia Treatment
- Cell death mechanisms and regulation
- Chronic Kidney Disease and Diabetes
- Vitamin D Research Studies
- Lipid metabolism and disorders
- Veterinary Pharmacology and Anesthesia
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Telomeres, Telomerase, and Senescence
- Advanced Drug Delivery Systems
- Cannabis and Cannabinoid Research
- Vaccine Coverage and Hesitancy
- Neonatal Health and Biochemistry
University of Pennsylvania
2016-2025
Children's Hospital of Philadelphia
2023-2025
Baylor College of Medicine
2021-2023
Texas Children's Hospital
2021-2023
Case Western Reserve University
2015
University of Puerto Rico-Mayaguez
2013
Rutgers, The State University of New Jersey
2013
Significance Extensive studies have detailed the molecular regulation of individual components hemostatic system. Important questions remain, however, regarding how elements coagulation cascade, platelets, and vessel wall are integrated at systems level to achieve hemostasis in vivo. Here, a series high-resolution imaging studies, we explore detail plug composed platelets fibrin forms setting penetrating vascular injuries. The results reveal surprisingly heterogeneous architecture, including...
Current cancer therapies are challenged by weakly soluble drugs and drug combinations that exhibit non-uniform biodistribution poor bioavailability. In this study, we have presented a new platform of advanced healthcare materials based on albumin nanoparticles (ANPs) engineered as tumor penetrating, delivery vehicles combinatorially applied factors to solid tumors. These were designed overcome three sequential key barriers: tissue level transport across matrix; uptake kinetics into...
Abstract Hemostasis is an innate protective mechanism that plays a central role in maintaining the homeostasis of vascular system during injury. Studying this essential physiological process often challenged by difficulty modeling and probing complex dynamics hemostatic responses native context human blood vessels. To address major challenge, paper describes microengineering approach for vitro hemostasis. This microphysiological model replicates living endothelium, multilayered...
Abstract Ku70 (Lupus Ku autoantigen p70) is essential in nonhomologous end joining DNA double-strand break repair, and ku70 −/− mice age prematurely because of increased genomic instability damage responses. Previously, we found that also inhibits Bax, a key mediator apoptosis. We hypothesized Bax-mediated apoptosis would be enhanced the absence contribute to premature death observed mice. Here, show bax +/− have better survival, especially females, than mice, even though Bax deficiency did...
Abstract Background Accurate assessment of kidney function is essential for early detection damage. While measured glomerular filtration rate (mGFR) occasionally used as a reference, estimated GFR (eGFR) from serum creatinine- and cystatin C (CysC)-based equations are routinely in clinical practice reliable less invasive approach. In pediatric populations, CysC-based provide closer approximation they independent body composition. Limited information available on the performance comparison...
Abstract Introduction Detection of iron deficiency (ID) remains challenging. We aimed to evaluate the performance reticulocyte hemoglobin equivalent (Ret‐He) as a potential diagnostic marker assess ID and anemia (IDA) in large pediatric cohort. Methods A total 3158 patients (aged 15 days 19 years with median age 8.5 years; 60.2% female) were retrospectively studied. Statistical analysis was performed (a) relationship Ret‐He other relevant complete blood count panel parameters; (b) compare...
Eltrombopag is a thrombopoietin-receptor agonist used to restore platelet count hemostatic levels in chronic immune thrombocytopenia. The drug has shown have hepatobiliary adverse effects, but also positive interference with the analytical measurement of bilirubin. Understanding degree this bilirubin testing becomes relevant clinical management these patients.Eltrombopag at concentrations ranging from 10 150 µg/ml was spiked into plasma samples different baseline Total bilirubin, conjugated,...
Abstract Background Vitamin D toxicity is rare in pediatric population. Falsely elevated levels of 25‐hydroxyvitamin have been reported as a major challenge with immunoassay methods for quantifying vitamin metabolites. Case Presentation and Method Here, we present two cases falsely that resulted unnecessary further testing. We also report significant same‐day variation the measurement using Abbott i2000SR immunoassay. Samples were spun twice their values confirmed gold standard liquid...
Abstract Background Ionized calcium monitoring is crucial for critically ill patients as well undergoing apheresis. According to CLSI C31-A2:2001, the acceptable stability time all tests be run on blood gas analyzers in heparinized syringes thirty minutes from collection. We sought determine whether of ionized samples can extended accommodate prolonged transport times. Methods Sixty whole were collected into 1CC containing 23 IU heparin (Vital Signs, Inc.) and sent laboratory pH analysis...
Abstract Background Total carbon dioxide (tCO2) measurements are important in the assessment of acid-base disorders. The collection and storage conditions specimens preanalytical factors that can influence accuracy tCO2 measurements. Proficiency testing (PT) materials used to assess reliability a laboratory’s test performance. Unsuccessful performance on PT challenges be an indicator procedural issues. However, stability material compared physiologic is unknown no guidance provided...
Abstract Background In the U.S., fentanyl mixed with xylazine has been designated as an emerging health threat. Information on whether is present poisoning could help better navigate management of children exposed to xylazine. The detection can be a marker used differentiate medical vs non-medical exposure. We aimed explore trends in positivity rates and/or at urban pediatric tertiary care hospital. Methods conducted retrospective analysis from October 2021 - January 2024 positive urine drug...
Atovaquone has traditionally been used as an antiparasitic and antifungal agent, but recent studies have shown its potential anticancer agent. The high variability in atovaquone bioavailability highlights the need for therapeutic drug monitoring, especially pediatric patients. goal of our study was to develop validate performance assay quantify plasma concentrations collected from cancer patients using LC-MS/MS. extracted a 10 µL volume K2-EDTA human solution consisting ACN: EtOH: DMF (8:1:1...
To gain insights on the heterogeneity of immune responses to vaccination against SARS-CoV-2 and identify factors that could make individuals vulnerable infection due lack response vaccination, our hospital started offering free voluntary post-antibody testing spike protein IgG for all fully vaccinated employees. Post-vaccination was assessed using FDA-EUA approved VITROS anti-SARS-CoV-2 immunometric assay specific protein. Out a total 3266 antibody tests performed in Texas Children's, 99.4%...
Abstract Background Given the recent legalization of recreational and medical marijuana in different states, there is an increasing exposure children teenagers to products which has led increase health care visits hospitalizations. Our drug testing algorithm uses immunoassay test for initial urinary screening (UDS) followed by automatic confirmatory only when a positive UDS result obtained. The performed in-house detects major metabolites delta9- tetrahydrocannabinol (THC), main psychoactive...
Previous studies using intravital microscopy have shown that hemostatic plugs formed in the mouse microvasculature a characteristic architecture which extent of platelet activation reflects gradients distribution agonists radiating outwards from injury site. In setting, we found minimal overlap thrombin and ADP signaling, with primarily responsible for robust close to site P2Y 12 -mediated signaling resulting accumulation minimally activated platelets. Here taken these major step forward by...
We have previously shown in the microcirculation that gradients of platelet agonists develop during hemostatic response as a consequence solute transport properties within evolving mass. These agonist result formation gradient activation extending from site vascular injury. Here we utilized combined light and electron microscopy approach to examine organization plugs formed larger injuries significant bleeding mouse jugular vein. The results show vessel wall puncture large vein is similarly...
Endogenous anticoagulants, including antithrombin III, tissue factor pathway inhibitor, and activated protein C (aPC) provide a biochemical means to inhibit thrombin generation and/or activity. In addition, we have previously shown that platelet mass retraction prevents the escape exchange of solutes, thereby providing biophysical mechanism limit The relative contribution each these mechanisms termination activity during hemostatic response in vivo remains poorly understood. Here, using...