A5024083306- Uterine Myomas and Treatments
- Ovarian cancer diagnosis and treatment
- Cancer Cells and Metastasis
- Sarcoma Diagnosis and Treatment
- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Cancer-related gene regulation
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Cancer-related Molecular Pathways
- Cancer Immunotherapy and Biomarkers
- Immunotherapy and Immune Responses
- Metastasis and carcinoma case studies
- Cancer Research and Treatments
- Endometrial and Cervical Cancer Treatments
- DNA Repair Mechanisms
- Kruppel-like factors research
- Peptidase Inhibition and Analysis
- Mechanisms of cancer metastasis
- Single-cell and spatial transcriptomics
- Telomeres, Telomerase, and Senescence
- Genetic factors in colorectal cancer
- TGF-β signaling in diseases
- Cancer-related molecular mechanisms research
Inserm
2014-2024
Université Claude Bernard Lyon 1
2014-2024
Centre Léon Bérard
2014-2024
Centre de Recherche en Cancérologie de Lyon
2014-2024
Centre National de la Recherche Scientifique
2012-2024
Université de Lyon
2023
Institut Curie
2021
Recently, two novel concepts have emerged in cancer biology: the role of so-called "cancer stem cells" tumor initiation, and involvement an epithelial-mesenchymal transition (EMT) metastatic dissemination epithelial cells. Using a mammary progression model, we show that cells possessing both tumorigenic characteristics can be derived from human following activation Ras-MAPK pathway. The acquisition these characters is driven by EMT induction.
The epithelial-mesenchymal transition (EMT) is an embryonic transdifferentiation process consisting of conversion polarized epithelial cells to motile mesenchymal ones. EMT–inducing transcription factors are aberrantly expressed in multiple tumor types and known favor the metastatic dissemination process. Supporting oncogenic activity within primary lesions, TWIST ZEB proteins can prevent from undergoing oncogene-induced senescence apoptosis by abolishing both p53- RB-dependent pathways....
Lower<sup>65</sup>Cu/<sup>63</sup>Cu ratios in the serum of colorectal and breast cancer patients relative to healthy individuals have potential diagnostic value.
Abstract Claudin-low breast cancers are aggressive tumors defined by the low expression of key components cellular junctions, associated with mesenchymal and stemness features. Although they generally considered as most primitive malignancies, their histogenesis remains elusive. Here we show that this molecular subtype exhibits a significant diversity, comprising three main subgroups emerge from unique evolutionary processes. Genetic, gene methylation analyses reveal two relate,...
TGF-β signaling is involved in pancreatic ductal adenocarcinoma (PDAC) tumorigenesis, representing one of the four major pathways genetically altered 100% PDAC cases. exerts complex and pleiotropic effects cancers, notably via activation SMAD pathways, predominantly SMAD2/3/4. Though SMAD2 3 are rarely mutated SMAD4 lost about 50% PDAC, role SMAD2/3 a SMAD4-null context remains understudied. We herein provide evidence oncogenic effect response to TGF-β1 human cancer cells. report that...
Whether fibroblasts regulate immune response is a crucial issue in the modulation of inflammatory responses. Herein, we demonstrate that foreskin (FFs) potently inhibit CD3+ T cell proliferation through mechanism involving early apoptosis activated cells. Using blocking antibodies, inhibition occurs cell-to-cell interactions implicating PD-1 receptor expressed on cells and its ligands, PD-L1 PD-L2, fibroblasts. Dual ligand neutralization required to abrogate (i) binding PD-1-Fc fusion...
The yeast yCCR4 factor belongs to the CCR4-NOT transcriptional regulatory complex, in which it interacts, through its leucine-rich repeat (LRR) motif with yPOP2. Recently, was shown be a component of major cytoplasmic mRNA deadenylase and contain fold related Mg2+-dependent endonuclease core. Here, we report identification nineteen yCCR4-related proteins eukaryotes (including yeast, plants animals), all endonuclease-like fold, highly conserved CCR4-specific residues. Phylogenetic genomic...
We have reported previously the physical interaction of B-cell translocation gene proteins (BTG)1 and BTG2 with mouse protein CAF1 (CCR4-associated factor 1) suggested that these may participate, through their association CAF1, in transcription regulation. Here we describe vitro vivo hPOP2, human paralog hCAF1. The functional relationships between BTG partners hCAF1 hPOP2 were investigated to find out how interactions affect cellular processes, particular defined regions examined expression...
In breast cancers, aberrant activation of the RAS/MAPK pathway is strongly associated with mesenchymal features and stemness traits, suggesting an interplay between this mitogenic signaling epithelial-to-mesenchymal plasticity (EMP). By using inducible models human mammary epithelial cells, we demonstrate herein that oncogenic RAS promotes ZEB1-dependent EMP, which necessary for malignant transformation. Notably, EMP triggered by secretion pro-inflammatory cytokines from neighboring...
The CCR4-associated protein CAF1 has been demonstrated to play several roles in the control of transcription and mRNA decay. To gain further insight into its physiological function, we generated CAF1-deficient mice. They are viable, healthy, normal appearance; however, mCAF1(-/-) male mice sterile. crossing mCAF1(+/-) gave a Mendelian ratio mCAF1(+/+), mCAF1(+/-), pups, indicating that haploid mCAF1-deficient germ cells differentiate normally. onset defect occurs during first wave...
Exposure of mammalian cells to genotoxic agents evokes a complex cellular response. An ordered series molecular events is necessary sense DNA damage, transduce the signal, and ultimately delay cell cycle or trigger apoptosis. Recently, we have shown that BTG2/TIS21 gene expression was induced in response damage through p53-dependent pathway. This belongs newly identified family structurally related genes whose other known human members are BTG1, BTG3, Tob. To define respective involvement...
Modulation of microRNAs (miRNAs) expression in many types cancer suggests that they may be involved crucial steps during tumour progression. Indeed, miRNAs deregulation has been described pituitary tumourigenesis, but few studies their role progression towards aggressiveness and malignancy. To assess the miRNA within hierarchical events cascade occurring prolactin (PRL) tumours malignancy, we used an integrative genomic approach associating clinic-pathological features, global transcriptomic...
<h2>Abstract</h2> Breast cancer is one of the most prominent types cancers, in which therapeutic resistance a major clinical concern. Specific subtypes, such as claudin-low and metaplastic breast carcinoma (MpBC), have been associated with high nongenetic plasticity, can facilitate resistance. The similarities differences between these orthogonal identified by molecular histopathological analyses, respectively, remain insufficiently characterized. Furthermore, adequate methods to identify...
Tumor-initiating cells (TICs), aka "cancer stem cells", are believed to fuel tumors and sustain therapy resistance systemic metastasis. Breast cancer is the first human carcinoma in which a subpopulation of displaying specific CD44+/CD24-/low/ESA+ antigenic phenotype was found have TIC properties. However, not universal marker TICs all breast subtypes. The aim this study identify novel antigens with isolate population basal-A/basal-like cell lines. We used polychromatic flow-cytometry...
The yeast CCR4-NOT complex exists in two forms (1.0 and 1.9 MDa) that share several common subunits, including yCCR4, yCAF1 five NOT proteins (NOT1-5). Here, we report different complexes containing mammalian homologs of subunits exist cells, with estimated sizes approximately MDa, 1 MDa 650 kDa, BTG2, a member protein family antiproliferative functions, can associate these complexes. Immunoprecipitation gel filtration experiments established BTG2 interacts vivo hCCR4 via hCAF1 hPOP2....
DNA methylation is thought to induce transcriptional silencing through the combination of two mechanisms: repulsion activators unable bind their target sites when methylated, and recruitment repressors with specific affinity for methylated DNA. The Methyl CpG Binding Domain proteins MeCP2, MBD1 MBD2 belong latter category. Here, we present ChIPseq data obtained from endogenous in an isogenic cellular model oncogenic transformation human mammary cells. In immortalized (HMEC-hTERT) or...