A5050139203- Microbial Natural Products and Biosynthesis
- Carbohydrate Chemistry and Synthesis
- Genomics and Phylogenetic Studies
- Mycobacterium research and diagnosis
- Chemical Synthesis and Analysis
- Marine Sponges and Natural Products
- Synthetic Organic Chemistry Methods
- Enzyme Production and Characterization
- Infectious Diseases and Mycology
- Parkinson's Disease Mechanisms and Treatments
- Tryptophan and brain disorders
- Plant-Microbe Interactions and Immunity
- Gut microbiota and health
- Fungal Biology and Applications
- Plant Pathogens and Fungal Diseases
- Gastrointestinal motility and disorders
- Cancer therapeutics and mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Tuberculosis Research and Epidemiology
- Synthesis and Biological Evaluation
- Fluorine in Organic Chemistry
- Enzyme Structure and Function
- Enzyme Catalysis and Immobilization
- Phytochemistry and Bioactive Compounds
- Bioactive Compounds and Antitumor Agents
Shangrao Normal University
2025
Jiangnan University
2021-2024
University of Cambridge
2007-2019
Monash University
2008
Ohio University
1995
Cleveland Clinic
1993
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTIrreversible inactivation of macrophage and brain nitric oxide synthase by L-NG-methylarginine requires NADPH-dependent hydroxylationPaul L. Feldman, Owen W. Griffith, Hui Hong, Dennis J. StuehrCite this: Med. Chem. 1993, 36, 4, 491–496Publication Date (Print):February 1, 1993Publication History Published online1 May 2002Published inissue 1 February 1993https://doi.org/10.1021/jm00056a009RIGHTS & PERMISSIONSArticle...
Accumulative studies suggest that inflammatory bowel disease (IBD) may cause multiple central nervous system (CNS) pathologies. Studies have found indoleamine-2,3-dioxygenase (IDO, rate-limiting enzyme of the kynurenine (Kyn) pathway) deficient mice were protected from endotoxin induced cognitive impairment, and Kyn administration memory deficits in both control IDO-deficient mice. However, there is no investigation brain pathway IBD, thus we investigated whether dextran sulfate sodium...
JOURNAL/nrgr/04.03/01300535-202508000-00026/figure1/v/2024-09-30T120553Z/r/image-tiff Interferon regulatory factor 7 plays a crucial role in the innate immune response. However, whether interferon 7-mediated signaling contributes to Parkinson’s disease remains unknown. Here we report that is markedly up-regulated 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine–induced mouse model of and co-localizes with microglial cells. Both selective cyclic guanosine monophosphate adenosine synthase...
Buruli ulcer (BU) is a progressive disease of subcutaneous tissues caused by Mycobacterium ulcerans. The pathology BU lesions associated with the local production diffusible substance, mycolactone, cytocidal and immunosuppressive properties. defective inflammatory responses in reflect these biological properties toxin. However, whether mycolactone diffuses from infected suppresses IFN-gamma patients remains unclear. Here we have investigated pharmacodistribution following injection animal...
The 174-kb virulence plasmid pMUM001 in Mycobacterium ulcerans epidemic strain Agy99 harbors three very large and homologous genes that encode giant polyketide synthases (PKS) responsible for the synthesis of lipid toxin mycolactone. Deeper investigation M. resulted identification two types spontaneous deletion variants within a population cells also contained intact plasmid. These arose from recombination between 8-kb sections same sequence, resulting loss 65-kb region bearing mycolactone...
The biosynthetic pathway to the unusual tetronate ring of certain polyketide natural products, including antibiotics abyssomicin and tetronomycin (TMN) antitumour compound chlorothricin (CHL), is presently unknown. gene clusters governing biosynthesis both contain a encoding an atypical member FkbH family enzymes, which has previously been shown synthesise glyceryl-S-acyl carrier protein (ACP) as first step in production extender units for modular biosynthesis. We show here that purified...
Lasalocid is a highly atypical polyether ionophoric antibiotic, firstly because it contains type of aromatic ring normally associated with fungal polyketides, and secondly the formation its tetrahydropyran appears to contravene Baldwin's rules, which predict kinetically preferred routes for cyclisation reactions in organic chemistry. The lasalocid biosynthetic gene cluster has been cloned from Streptomyces lasaliensis, las locus (73,533 bp) was found contain seven modular polyketide synthase...
Say "when": Analysis of the biosynthetic gene cluster for polyether antibiotic and anticancer agent salinomycin (1) shows that its core structure is synthesised by a nine-multienzyme modular polyketide synthase. Deletion salC gene, which required oxidative cyclisation, has led to detection novel metabolite whose reveals timing key dehydration step. Detailed facts importance specialist readers are published as "Supporting Information". Such documents peer-reviewed, but not copy-edited or...
The identity and reactivity of the intermediates in agglomerin biosynthesis were established respective roles acetyltransferase Agg4 eliminating enzyme Agg5 identified (see scheme). It is proposed that enzymes homologous to carry out dehydration steps all spirotetronate biosynthetic pathways. If this proves correct, it may assist engineering these As a service our authors readers, journal provides supporting information supplied by authors. Such materials are peer reviewed be re-organized...
Keeping it basic: Arginine provides the exotic 4-guanidinobutanoate starter unit for two different types of zwitterionic polyketide (an example one type is shown in picture) produced by same Streptomyces bacterium. The three-step precursor pathway initiated a remarkable decarboxylating monooxygenase with high specificity arginine. As service to our authors and readers, this journal supporting information supplied authors. Such materials are peer reviewed may be re-organized online delivery,...
An <italic>in vitro</italic> model system based on a ketosynthase domain of the erythromycin polyketide synthase was used to probe apparent substrate tolerance domains mycolactone synthase.
Abstract Phosphopantetheinyl transferases (PPTases) catalyze the essential post‐translational activation of carrier proteins (CPs) from fatty acid synthases (FASs) (primary metabolism), polyketide (PKSs), and non‐ribosomal polypeptide synthetases (NRPSs) (secondary metabolism). Bacteria typically harbor one PPTase specific for CPs primary metabolism (“ACPS‐type” PPTases) at least capable modifying involved in secondary (“Sfp‐type” PPTases). In order to identify PPTase(s) associated with...
Abstract Polyether ionophores, such as monensin A, are known to be biosynthesised, like many other antibiotic polyketides, on giant modular polyketide synthases (PKSs), but the intermediates and enzymes involved in subsequent steps of oxidative cyclisation remain undefined. In particular there has been no agreement mechanism timing final chain release. We now report evidence that MonCII from biosynthetic gene cluster Streptomyces cinnamonensis , which was previously thought an epoxide...
New analogues of the toxin mycolactone have been identified in a pathogenic Chinese strain Mycobacterium ulcerans. They possess an extra methyl group at C2′ (shown red) compared to A as result recruitment single catalytic domain altered specificity polyketide synthase.
Abstract Desertomycin A is an aminopolyol polyketide containing a macrolactone ring. We have proposed that desertomycin and similar compounds (marginolactones) are formed by synthases primed not with γ‐aminobutanoyl‐CoA but 4‐guanidinylbutanoyl‐CoA, to avoid facile cyclization of the starter unit. This hypothesis requires there be final‐stage de‐amidination corresponding guanidino‐substituted natural product, no enzyme for such process has been described. now identified candidate...
A mild and effective visible-light-induced decarboxylative radical cascade reaction of olefin-containing imidazoles α-fluorinated carboxylic acids, has been developed to afford monofluoromethylated or aryldifluoromethylated polycyclic imidazoles.
LC-MSn analysis of mycolactone toxin from extracts Mycobacterium ulcerans has shown that minor co-metabolites, including two previously unreported, differ structurally only in a small portion the polyketide side-chain.
The function of gene products involved in the biosynthesis clinically important polyketide rapamycin were elucidated by biotransformation and complementation