A5042266507- Monoclonal and Polyclonal Antibodies Research
- PARP inhibition in cancer therapy
- Advanced Breast Cancer Therapies
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Cell Adhesion Molecules Research
- Retinoids in leukemia and cellular processes
- Immunotherapy and Immune Responses
- Asthma and respiratory diseases
- HER2/EGFR in Cancer Research
- Systemic Lupus Erythematosus Research
- Complement system in diseases
- Estrogen and related hormone effects
- Cytokine Signaling Pathways and Interactions
- Peripheral Neuropathies and Disorders
- Pregnancy-related medical research
- Photoacoustic and Ultrasonic Imaging
- Retinal Diseases and Treatments
- Glycosylation and Glycoproteins Research
- Lymphoma Diagnosis and Treatment
- Immune cells in cancer
- Erythrocyte Function and Pathophysiology
- Photodynamic Therapy Research Studies
- Acute Lymphoblastic Leukemia research
- Rheumatoid Arthritis Research and Therapies
Io Therapeutics (United States)
2014-2024
Opexa Therapeutics (United States)
2024
Ark Therapeutic (United States)
2021
Vertex Pharmaceuticals (United States)
2014-2021
Humanetics Corporation (United States)
2009
Robarts Clinical Trials
2005
University of Alabama at Birmingham
1993-2000
Boston University
1987-2000
Wayne State University
2000
University of California, Los Angeles
2000
Abstract Studies of cell-surface molecules involved in human T cell interaction reveal that differential expression each three adhesion (LFA-3, CD2, and LFA-1) subdivides peripheral blood cells into major subpopulations. Systematic analysis the relationship between these other markers subsets demonstrates a single subset lymphocytes distinguished by enhanced LFA-3, LFA-1, (CDw29 [4B4], UCHL1, Pgp-1). Large differences relative are observed for UCHL1 (29-fold) LFA-3 (greater than 8-fold),...
Natalizumab, a humanized monoclonal antibody against alpha4 integrin, inhibits leukocyte adhesion and migration into inflamed tissue.We conducted two controlled trials to evaluate natalizumab as induction maintenance therapy in patients with active Crohn's disease. In the first trial, 905 were randomly assigned receive 300 mg of or placebo at weeks 0, 4, 8. The primary outcome was response, defined by decrease Disease Activity Index (CDAI) score least 70 points, week 10. second 339 who had...
Although intercellular adhesion molecule-1 (ICAM-1) has been implicated as a ligand in some LFA-1-dependent adhesion, its importance to T cell function not established. The present studies investigate the of ICAM-1 for human cytotoxic lymphocytes (CTL), both their formation antigen-independent conjugates (AIC) and lysis targets. Analysis monoclonal antibody (mAb) inhibition AIC indicate that mAb 1 blocks (a) with but all targets; (b) LFA-1 pathway CD2/LFA-3 adhesion; (c) by binding target...
Background: Relaxin is a pregnancy-related hormone that has tissue remodeling and antifibrotic effects. Systemic sclerosis (scleroderma) characterized by fibrosis of the skin, vasculature, internal organs. Objective: To assess efficacy, safety, dose–response effect recombinant human relaxin in patients with scleroderma. Design: Multicenter, parallel-group, randomized, double-blind, placebo-controlled trial. Setting: Academic referral centers. Patients: 68 who had stable, diffuse scleroderma...
CD2 is a T lymphocyte glycoprotein that functions in adhesion of lymphocytes and also as putative receptor for activation signals. Functional data suggest LFA-3, widely distributed cell surface glycoprotein, may be the biological ligand CD2. We have purified LFA-3 from human erythrocytes characterized protein functionally. bound specifically to CD2+ cells, this binding was inhibited by mAb. Conversely, mAb concentration required effect suggests half-saturated at 1-5 nM LFA-3. Purified...
Abstract A quantitative enzyme-linked immunosorbent assay was used to measure the concentration of fluid-phase complement C5b-9 complexes (SC5b-9) in cerebrospinal fluid (CSF) 14 patients with acute monophasic Guillain-Barré Syndrome (GBS), 21 multiple sclerosis (MS), and 11 noninflammatory central nervous system (CNS) diseases. SC5b-9 were detected CSF 13 GBS (mean, 3.08 micrograms/ml; range, 0 7.1 micrograms/ml) 16 MS 1.83 7.5 micrograms/ml). In control group CNS diseases, not eight...
Abstract Objective A phase II randomized controlled trial of recombinant human relaxin suggested that a dosage 25 μg/kg/day was safe and clinically effective in improving skin disease reducing functional disability scleroderma (systemic sclerosis; SSc). We undertook large randomized, double‐blind, placebo‐controlled clinical to compare placebo with 10 relaxin, given for 24 weeks patients stable, diffuse, moderate‐to‐severe SSc. Methods Men women ages 18–70 years diffuse cutaneous SSc (dcSSc)...
In the present study, role of antiperipheral nerve myelin antibody (anti-PNM Ab) in demyelination by generating terminal attack complex (C5b-9) complement was explored patients with Guillain-Barré syndrome (GBS) and other demyelinating neuropathies. The presence serum SC5b-9, an inactive C5b-9 containing S protein, assessed quantitatively enzyme-linked immunosorbent assay using (Ab) to neoantigens expressed on C9 when complexed C5b-8 or after tubular polymerization. SC5b-9 detected all 19...
We have isolated the cDNA for human lymphocyte function-associated antigen 3 (LFA-3), ligand of T CD2 molecule. The identity clones was established by comparison deduced amino acid sequence to LFA-3 NH2-terminal and tryptic peptide sequences. defines a mature protein 222 acids that structurally resembles typical membrane-anchored proteins. An extracellular domain with six N-linked glycosylation sites is followed hydrophobic putative transmembrane region short cytoplasmic domain. glycoprotein...
Abstract This study was undertaken in an effort to understand the role of cytokines T lymphocyte trafficking into inflamed synovium and potential enhancement antigen presentation by human synovial fibroblasts. We found that interleukin‐1β (IL‐1β), tumor necrosis factor α (TNFα), interferon‐γ (IFNγ) each increased cell surface expression intercellular adhesion molecule 1 (ICAM‐1) on fibroblasts a dose‐ time‐dependent manner. Maximal ICAM‐1 occurred within 8 hours induction, with following...
CD2, also known as LFA-2, T11, and the E rosette receptor, is a T lymphocyte surface protein functionally important in adhesion to target cells cell triggering. LFA-3 widely distributed that functions on cells. We find expressed human E, CD2 receptor for mediates E. Pretreatment of lymphocytes with mAb or inhibits rosetting. Purified molecules bind inhibit 125I-CD2 binding inhibited by mAb; reciprocally, pretreatment purified CD2. Higher concentrations aggregate E; aggregation LFA-3.
Previous investigations have defined phenotypic differences between unprimed (naive) and antigen-primed (memory) T cells from human peripheral blood. We now report that memory proliferate much more than naive when stimulated with anti-CD3 monoclonal antibody or pairs of anti-CD2 antibodies. Enhanced responsiveness to receptor-mediated triggering is a novel mechanism for which could facilitate cell response specific antigen. Furthermore, triggered via either CD2 CD3, produce substantial...
Abstract Objectives. To evaluate the safety, immunogenicity, and biologic effects of chimeric monoclonal anti‐CD4 (cM‐T412) in patients with refractory rheumatoid arthritis (RA), to obtain preliminary data on clinical response this treatment. Methods. Twenty‐five active RA were treated incremental doses (10 700 mg) cM‐T412 an open‐label, escalating‐dose phase I trial. Results. Infusion was followed by immediate, rapid decline CD4+ T cells. The level circulating cells remained depressed most...
Abstract Objective. To investigate the effects of chimeric CD4 monoclonal antibody cM‐T412 treatment in patients with rheumatoid arthritis (RA). Methods. Thirty‐two RA received daily doses 10, 50, or 100 mg intravenously for 7 days. Results. There was a sustained decrease number CD4+ T lymphocytes all patients. Those who 50 and experienced significant reductions disease activity. Conclusion. Treatment appears to have dose‐dependent beneficial effect The clinical are independent depressed...
THE recent independent observation, by several of us, peculiar histiocytosis the spleens patients with "idiopathic" thrombocytopenic purpura has prompted collection and review material here presented. The 9 patients†† described have shown no consistent abnormality other blood elements, unusual histiocytes appear limited to spleen. Only 2 had onset symptoms in middle life, as did similar ones literature.1 , In majority developed childhood or early adult life. Easy bruising, repeated epistaxis...
// Jun Wang 1,2 , Weina Bi 1 Wei Zhao Merina Varghese Rick J. Koch 3 Ruth H. Walker Roshantha A. Chandraratna 4 Martin E. Sanders Amanda Janesick 5 Bruce Blumberg Libby Ward Lap Ho and Giulio M. Pasinetti Department of Neurology, Icahn School Medicine at Mount Sinai, New York, NY, USA 2 Geriatric Research, Education Clinical Center, Bronx, James Peters Veterans Affairs Medical Io Therapeutics Inc., Santa Ana, CA, Departments Developmental Cell Biology, Pharmaceutical Sciences, University...
We studied the molecular composition of complement C5b-9 complex required for optimal killing Escherichia coli strain J5. J5 cells were incubated in 3.3%, 6.6%, or 10.0% C8-deficient serum previously absorbed to remove specific antibody and lysozyme. This resulted stable deposition after washing 310, 560, 890 C5b67 molecules per colony-forming unit, respectively, as determined by binding 125I-labeled C7. Organisms then with excess C8 various amounts 131I-labeled C9. Plots logarithm (base 10)...