A5006517672- Immune cells in cancer
- Ocular Diseases and Behçet’s Syndrome
- Neuroinflammation and Neurodegeneration Mechanisms
- Systemic Lupus Erythematosus Research
- Sphingolipid Metabolism and Signaling
- interferon and immune responses
- Ferroptosis and cancer prognosis
- Immune Cell Function and Interaction
- Diet and metabolism studies
- T-cell and B-cell Immunology
- Telomeres, Telomerase, and Senescence
- Immune Response and Inflammation
- Mitochondrial Function and Pathology
- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- RNA Interference and Gene Delivery
- COVID-19 Clinical Research Studies
- Phagocytosis and Immune Regulation
- Chemokine receptors and signaling
- Inflammasome and immune disorders
- Nuclear Receptors and Signaling
- MicroRNA in disease regulation
- Adipose Tissue and Metabolism
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
University of Pennsylvania
2021-2025
NYU Langone Health
2021
New York University
2016-2017
Senescence is a cellular state linked to ageing and age-onset disease across many mammalian species
Hematopoietic stem cell transplantation (HSCT) can replace endogenous microglia with circulation-derived macrophages but has high mortality. To mitigate the risks of HSCT and expand potential for replacement, we engineered an inhibitor-resistant CSF1R that enables robust replacement. A glycine to alanine substitution at position 795 human (G795A) confers resistance multiple inhibitors, including PLX3397 PLX5622. Biochemical cell-based assays show no discernable gain or loss function. G795A-...
Monogenic blood diseases are among the most common genetic disorders worldwide. These result in significant pediatric and adult morbidity, some can death prior to birth. Novel ex vivo hematopoietic stem cell (HSC) gene editing therapies hold tremendous promise alter therapeutic landscape but not without potential limitations. In offer a potentially safer more accessible treatment for these hindered by lack of delivery vectors targeting HSCs, which reside difficult-to-access bone marrow...
Microglia, the brain’s resident macrophages, can be reconstituted by surrogate cells - a process termed “microglia replacement.” To expand microglia replacement toolkit, we here introduce estrogen-regulated (ER) homeobox B8 (Hoxb8) conditionally immortalized cell model for generation of immune from murine bone marrow, as versatile replacement. We find that ER-Hoxb8 macrophages are highly comparable to primary marrow-derived (BMD) in vitro, and, when transplanted into microglia-free brain,...
Microglia, the brain’s resident macrophages, can be reconstituted by surrogate cells - a process termed “microglia replacement.” To expand microglia replacement toolkit, we here introduce estrogen-regulated (ER) homeobox B8 (Hoxb8) conditionally immortalized cell model for generation of immune from murine bone marrow, as versatile replacement. We find that ER-Hoxb8 macrophages are highly comparable to primary marrow-derived (BMD) in vitro, and, when transplanted into microglia-free brain,...
We define a subset of macrophages in the tumor microenvironment characterized by high intracellular iron and enrichment heme metabolism genes. These iron-rich tumor-associated (iTAMs) supported angiogenesis immunosuppression were conserved between mice humans. iTAMs comprise two additional subsets based on gene expression profile location—perivascular (pviTAM) stromal (stiTAM). identified endothelin receptor type B (Ednrb) as specific marker found myeloid-specific deletion Ednrb to reduce...
Microglia, the brain's resident macrophages, can be reconstituted by surrogate cells - a process termed "microglia replacement." To expand microglia replacement toolkit, we here introduce estrogen-regulated (ER) homeobox B8 (Hoxb8) conditionally immortalized cell model for generation of immune from murine bone marrow, as versatile replacement. We find that ER-Hoxb8 macrophages are highly comparable to primary marrow-derived (BMD) in vitro, and, when transplanted into microglia-free brain,...
<h3>Background</h3> Tumor associated macrophages (TAMs) frequently comprise the most abundant leukocyte population within tumor microenvironment (TME). TAMs are phenotypically diverse and can be polarized towards either pro- or anti-tumor functions by signals TME. While microvascular dysfunction with red blood cell (RBC) extravasation intra-tumoral hemorrhage hallmarks of solid tumors,<sup>1</sup> role RBCs heme in polarizing TME is not known. <h3>Methods</h3> We performed single RNA-seq...