5-Fluorouracil (5-Fu) is one of the most commonly used chemotherapy drugs for gastric cancer (GC). But increase drug resistance makes prognosis patients worse. Studies have shown that Baicalin can not only inhibit various cancers but also sensitivity to chemotherapy. However, how works in chemotherapeutic GC are unclear.CCK8 (Cell Counting Kit 8) was detect IC50 (half maximal inhibitory concentration) and 5-Fu. Proliferation, migration, invasion were tested through colony formation assay...

Long intergenic noncoding RNAs (lincRNAs) can regulate the transcription of inflammatory genes and thus may represent a new group mediators with potential pathogenic role in diseases. Here, our genome-wide transcriptomic data show that TNF-α stimulation caused upregulation 171 lincRNAs down-regulation 196 murine intestinal epithelial cells culture. One up-regulated lincRNAs, lincRNA-Cox2, is an early-responsive lincRNA induced by through activation NF-κB signaling pathway. Knockdown...

Cheng C, Lu X, Wang G, Zheng L, Shu Zhu S, Liu K, Wu Tong Q. Expression of SATB1 and heparanase in gastric cancer its relationship to clinicopathologic features. APMIS 2010; 118: 855–63. The purpose this study was investigate the expression special AT‐rich binding protein 1 (SATB1) human as well factors. Specimens from 102 patients who underwent radical gastrectomy between 2000 2002 were studied by immunohistochemistry for expression. positively expressed 48.0% 51.0% cases, respectively....

In previous a study, we had developed novel thermosensitive magnetic delivery system based on liposomes. This study aimed to evaluate the efficiency of this for co-delivery both drugs and genes same cell its anti-tumor effects gastric cancer. Doxorubicin (DOX) SATB1 shRNA vector were loaded into system, in vitro DOX release activity, targeted gene silencing efficiency, cellular uptake, cytotoxicity, as well vivo activity determined. The results showed that desirable efficacy, silencing....

Radiation therapy is commonly used for prostate cancer treatment; however, normal tissues can be damaged from the reactive oxygen species (ROS) produced by radiation. In separate reports, we and others have shown that manganese porphyrins (MnPs), ROS scavengers, protect cells radiation-induced damage but inhibit cell growth. However, there been no studies demonstrating MnPs tissues, while inhibiting tumor growth in same model. LNCaP or PC3 were orthotopically implanted into athymic mice...

Abstract Long noncoding RNAs are important regulators of gene expression in innate immune responses. Antisense IL-7 (IL-7–AS) is a newly discovered long RNA human and mouse that has been reported to regulate the IL-6. However, potential function IL-7–AS system not fully understood. In this study, we found primarily dependent on NF-κB MAPK signaling pathways macrophages intestinal epithelial cells. Functionally, promotes several inflammatory genes, including CCL2, CCL5, CCL7, IL-6, cells...

Background Lonicera macranthoides Hand.-Mazz., the primary sources of Lonicerae Flos(Shanyinhua), brings great medicinal and economic value as an invaluable source natural bioactive compounds. Nutrient metabolites accumulation generally changed accompany with its floral development opening. While specific pattern underlying molecular regulatory networks remain unclear. Methods The present study intergrated a comparative analysis upon UPLC-MS/MS-based metabolomics RNA-seq-based...

The purpose of this research is to evaluate the predictive capabilities a radiomics model for Ki67 index and its correlation with prognosis in advanced gastric tubular adenocarcinoma patients. Clinical data from 213 patients were analyzed, categorizing into high low groups. radiomic features 192 selected by lasso method was constructed, which validated using TCIA dataset. Univariate multivariate Cox regression analyses used further analyze clinical associated cancer, models are also assess...

Special AT-rich sequence binding protein 1 (SATB1) is a nuclear factor that functions as global chromatin organizer to regulate gene expression. Recent studies have suggested an oncogenic role of SATB1 in breast cancer. However, the gastric cancer, especially regulating malignant phenotypes, including multidrug resistance (MDR) and metastasis, remains poorly understood. In this study, aggressive human cancer cell line SGC7901 its corresponding MDR variant SGC7901/VCR cells were used model....

Glioma is one of the most common malignant central nervous system tumors in humans. Schisandrin B (Sch B) has been confirmed to cause proliferation and invasion glioma cells. In present study, potential mechanism underlying antitumor effect Sch on cells was investigated. The cell lines, U251 U87, were exposed B, viability, apoptosis, migration, determined using MTT assay, flow cytometry, transwell respectively. Then, effects HOTAIR miR-125a tumor biology mammalian target rapamycin (mTOR)...

The diagnosis rate of early stage esophageal squamous cell carcinoma (ESCC) is low due to the lack specific tumor markers. Seeking for these markers beneficial improve and prognosis patients. This study profiles differentially expressed proteins ESCC patients via AAH-BLG-507 protein chip, which further consolidates clinical evidence diagnosis.In this study, 20 serum samples were collected from Taihe Hospital between August 2016 June 2017. Ten them carried ESCC, while rest healthy controls....

Gastric cancer (GC) is the third leading cause of tumor related mortality worldwide. Peritoneal metastasis (PM) occurs in more than half advanced GC patients, to poor prognosis. Therefore, GSE62254 cohort was used construct a signature consisting four RNA-binding proteins (RBP) predict possibility PM patients. Then, ROC curves were plotted followed by calculation AUCs, showing that had similar predictive accuracy compared with TNM staging system. Importantly, capability prediction enhanced...

Objective. Development of drug and radiation resistance is one the major causes cancer treatment failure with chemoradiotherapy. Whether radiotherapy affects drugs in esophageal cells remain to be determined. The purpose study was investigate change drug-sensitivity P-glycoprotein (P-gp) expression ionization radiation-induced human radioresistant cells. Materials methods. Radioresistant were established by means continuous fractionated gamma-ray irradiation on squamous cell line EC9706....

// Elizabeth A. Kosmacek 1 , Arpita Chatterjee Qiang Tong Chi Lin 2 Rebecca E. Oberley-Deegan Department of Biochemistry and Molecular Biology, University Nebraska Medical Center, Omaha, NE, 68198, USA Radiation Oncology, Correspondence to: Oberley-Deegan, email: becky.deegan@unmc.edu Keywords: MnTnBuOE-2-PyP, radiation, fibroblast, colorectal cancer, oxidative stress Received: March 01, 2016 Accepted: 31, Published: April 22, ABSTRACT Manganese porphyrins have been shown to be potent...

Abstract Background and Objectives Regulatory T cells (Treg) inhibits immune responses mediated by cells. This study aimed to investigate whether Treg are accumulated in human gastric cancer tissue the mechanism of induction Methods Tissue infiltrated leukocytes from adenocarcinomas were subjected flow cytometry immunohistochemistry. Percentage, phenotype, function, clinical relevance analyzed. TGF‐β1 production was determined Western blot vitro co‐culture experiments performed mimic...

Metastasis and invasion are the main causes of mortality in gastric cancer. To improve treatment cancer, development effective innovative antitumor agents toward proliferation is needed. Alpha-lipoic acid (ALA), a naturally occurring thiol antioxidant, showed antiproliferative cytotoxic effects on several cancers. So it feasible to explore whether ALA can be used inhibit human The expression MUC4 cancer tissues was assayed by immunohistochemistry. Then, we performed vitro cell analysis...