A5103212949- Fungal and yeast genetics research
- DNA Repair Mechanisms
- Microtubule and mitosis dynamics
- Nerve injury and regeneration
- Angiogenesis and VEGF in Cancer
- Nuclear Structure and Function
- Cellular transport and secretion
- Genomics and Chromatin Dynamics
- Spinal Cord Injury Research
- Plant Reproductive Biology
- Cell Adhesion Molecules Research
- Natural product bioactivities and synthesis
- Endoplasmic Reticulum Stress and Disease
- Nerve Injury and Rehabilitation
- Neurogenesis and neuroplasticity mechanisms
- Platelet Disorders and Treatments
- Growth Hormone and Insulin-like Growth Factors
- Protease and Inhibitor Mechanisms
- Corneal Surgery and Treatments
- Bioactive natural compounds
- RNA Research and Splicing
- Lymphatic System and Diseases
- Genetic Neurodegenerative Diseases
- TGF-β signaling in diseases
- Connective Tissue Growth Factor Research
Wuhan Institute of Technology
2023
Sun Yat-sen University
2011-2021
Florida State University
2009-2020
Zhoukou City Academy of Agricultural Sciences
2017
Jingzhou Central Hospital
2015-2016
Institute of Hematology & Blood Diseases Hospital
2016
Zhongda Hospital Southeast University
2013
Tallahassee Orthopedic Clinic
2012
Dalian University of Technology
2012
Georgia State University
2011
CD34+ bone marrow-derived progenitor cells contribute to tissue repair by differentiating into endothelial cells, vascular smooth muscle hematopoietic and possibly other cell types. However, the mechanisms which circulating home remodeling tissues remain unclear. Here we show that integrin alpha4beta1 (VLA-4) promotes homing of ligands VCAM cellular fibronectin, are expressed on actively neovasculature. Progenitor express alpha4beta1, homed sites active tumor neovascularization but not...
Neovascularization depends on vascular cell proliferation and the stabilization of vessels by association smooth muscle-like pericytes with ECs. Here we show that integrin alpha4beta1 (VLA-4) VCAM-1 promote close intercellular adhesion between ECs this interaction is required for blood vessel formation. Integrin expressed proliferating but not quiescent ECs, while its ligand mural cells. Antagonists integrin-ligand pair block cells to endothelia in vitro vivo, thereby inducing apoptosis...
Ergosterol depletion independently inhibits two aspects of yeast mating: pheromone signaling and plasma membrane fusion. In signaling, ergosterol participates in the recruitment Ste5 to a polarized site on membrane. is thought form microdomains within by interacting with long acyl chains sphingolipids. We find that although sphingolipid-free concentrated at sites cell–cell contact, transmission signal contact depends balanced ratio If mating pair forms between ergosterol-depleted cells...
Abstract Monocytes and macrophages extensively colonize solid tumors, where they are thought to promote tumor angiogenesis. Here, we show that integrin α4β1 (VLA4) promotes the invasion of tumors by myeloid cells subsequent neovascularization. Antagonists α4β1, but not other integrins, blocked adhesion monocytes endothelium in vitro vivo as well their extravasation into tissue. These antagonists prevented monocyte stimulation angiogenesis vivo, macrophage colonization studies indicate...
During meiosis, homologues become juxtaposed and synapsed along their entire length. Mutations in the cohesin complex disrupt not only sister chromatid cohesion but also homologue pairing synaptonemal formation. In this study, we report that Pds5, a cohesin-associated protein known to regulate cohesion, is required for synapsis budding yeast. Pds5 colocalizes with length of meiotic chromosomes. absence subunit Rec8 remains bound chromosomes minor defects chromatids synapse instead...
Regulation of c-Fos transcription by GH is mediated CCAAT/enhancer binding protein β (C/EBPβ). This study examines the role C/EBPβ in mediating activation other early response genes, including Cyr61, Btg2, Socs3, Zfp36, and Socs1. depletion using short hairpin RNA impaired responsiveness these genes to GH, as seen for c-Fos. Rescue with wild-type led GH-dependent recruitment coactivator p300 promoter. In contrast, rescue mutated at ERK phosphorylation site T188 failed induce p300, indicating...
Severe spinal cord injury often causes temporary or permanent damages in strength, sensation, autonomic functions below the site of injury. So far, there is still no effective treatment for Mesenchymal stem cells (MSCs) have been used to repair injured as an strategy. However, low neural differentiation frequency MSCs has limited its application. The present study attempted explore whether grafted MSC-derived neural-like a gelatin sponge (GS) scaffold could maintain features...
The hostile environment of an injured spinal cord makes it challenging to achieve higher viability in a grafted tissue-engineered neural network used reconstruct the circuit. Here, we investigate whether cell survival and synaptic transmission within NT-3 TRKC gene-overexpressing stem cell-derived scaffold (NN) transplanted into transected could be promoted by electroacupuncture (EA) through improving microenvironment. Our results showed that EA facilitated survival, neuronal...
Abstract Aims This study was aimed to investigate whether electroacupuncture (EA) would increase the secretion of neurotrophin‐3 (NT‐3) from injured spinal cord tissue, and, if so, increased NT‐3 promote survival, differentiation, and migration grafted tyrosine kinase C ( TrkC )‐modified mesenchymal stem cell (MSC)‐derived neural network cells. We next sought determine latter integrate with host circuit improve neurological function cord. Methods After ‐modified Schwann cells (SCs) MSCs were...
Janus kinase 2 (JAK2), a tyrosine that associates with the GH receptor and is activated by GH, has been implicated as key mediator of signaling. Several published reports suggest members Src family kinases may also participate in We therefore investigated extent to which JAK2 mediate activation signal transducers activators transcription (STATs) 1, 3, 5a/b, ERKs 1 2, Akt, highly GH-responsive cell lines 3T3-F442A preadipocytes H4IIE hepatoma cells. was not detected either line. Further,...
Hypoxia, a reduction in partial oxygen pressure, is salient property of solid tumors. Hypoxia drives malignant progression and metastasis tumors participates tumor resistance to radio- chemotherapies. activates the hypoxia-inducible factor (HIF) family transcription factors, which induce target genes that regulate adaptive biological processes such as anaerobic metabolism, cell motility, angiogenesis. Clinical evidence has demonstrated expression HIF-1 strongly associated with poor patient...
Spinal cord injury (SCI) often results in death of spinal neurons and atrophy muscles which they govern. Thus, following SCI, reorganizing the lumbar sensorimotor pathways is crucial to alleviate muscle atrophy. Tail nerve electrical stimulation (TANES) has been shown activate central pattern generator (CPG) improve locomotion recovery contused rats. Electroacupuncture (EA) a traditional Chinese medical practice proven have neural protective effect. Here, we examined effects TANES EA on...
Aip3p/Bud6p is a regulator of cell and cytoskeletal polarity inSaccharomyces cerevisiae that was previously identified as an actin-interacting protein. Actin-interacting protein 3 (Aip3p) localizes at the cortex where cytoskeleton assembly must be achieved to execute polarized growth, deletion ofAIP3 causes gross defects in polarity. We have discovered Aip3p localization mediated by secretory pathway. Mutations early- or late-acting components apparatus lead mislocalization. Biochemical data...
The nucleus is enclosed by the inner nuclear membrane (INM) and outer (ONM). While ONM continuous with endoplasmic reticulum (ER), INM independent separates nucleoplasm from ER lumen. Turnover of proteins has been well characterized ER-associated protein degradation (ERAD) pathway, but very little known about turnover resident proteins. Here we show that anaphase-promoting complex/cyclosome (APC/C), an E3 ubiquitin ligase, regulates Mps3, a conserved integral INM. Mps3 requires...
Spinal cord injury (SCI) invariably results in neuronal death and failure of axonal regeneration. This is attributed mainly to the hostile microenvironment poor intrinsic regrowth capacity injured spinal neurons. We have reported previously that electro-acupuncture on Governor Vessel acupoints (GV-EA) can promote survival regeneration cord. However, underlying mechanism for this has remained uncertain. The present study aimed explore neural afferent pathway GV-EA stimulation possible by...
ABSTRACT Membrane fusion requires localized destabilization of two phospholipid bilayers, but unrestrained membrane could result in lysis. prm1 mutant yeast cells have a defect at the plasma stage mating that typically results accumulation prezygotes fingers membrane-bound cytoplasm projecting from one cell each pair into its partner direction osmotic gradient between cells. However, some pairs fuse successfully whereas other simultaneously lyse. Lysis only occurs if both partners are...
In budding yeast, the microtubule-organizing center is called spindle pole body (SPB) and shares structural components with centriole, central core of animal centrosome. During meiotic interphase I, SPB duplicated when DNA replication takes place. Duplicated SPBs are linked then separate to form a bipolar required for homolog separation in meiosis I. II, again, absence replication, four that establish two spindles sister-chromatid II. Here, we report Aurora kinase Ipl1, which necessary...
Worldwide, colon cancer is the third most common in terms of incidence, following lung and breast cancer. Resistance to psoralidin frequently occurs its use as an anticancer treatment. However, mechanisms underlying effects on cancer, remain be elucidated. Hence, present study investigated potential mechanism action SW480 human cells. In study, MTT assay was performed measure viability Additionally, Annexin V-fluorescein isothiocyanate/propidium iodide apoptosis detection kit, DAPI staining...
Mating yeast cells provide a genetically accessible system for the study of cell fusion. The dynamics fusion pores between were analyzed by following exchange fluorescent markers partners. Upon plasma membrane fusion, cytoplasmic GFP and DsRed diffuse at rates proportional to size pore. permeance measurements reveal that typical pore opens with burst then gradually expands. In some mating pairs, sudden increase in was found, consistent opening second contrast, other closed after permitting...
Previous work showed that the adapter protein SH2B 1beta (SH2B1) (SH2-B) binds to activated form of nerve growth factor (NGF) receptor TrkA and is critical for both NGF-dependent neurite outgrowth maintenance. To identify SH2B1beta-regulated genes outgrowth, we performed microarray analysis control PC12 cells stably overexpressing SH2B1beta (PC12-SH2B1beta) or dominant-negative SH2B1beta(R555E) [PC12-SH2B1beta(R555E)]. NGF-induced expression Plaur Mmp10 was greatly enhanced in PC12-SH2B1beta...
Vascular endothelial growth factor (VEGF) expression is up-regulated via a cyclooxygenase-2 (COX-2)-dependent mechanism in non-small cell lung cancer (NSCLC), but the specific signaling pathway involved unclear. Our aim was to investigate that links COX-2 with VEGF up-regulation NSCLC.COX-2 NSCLC samples detected immunohistochemically, and its association VEGF, microvessel density (MVD), other clinicopathological characteristics determined. The effect of treatment on proliferation cells...