A5102731703- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Wound Healing and Treatments
- Dermatologic Treatments and Research
- Aortic Disease and Treatment Approaches
- Cardiac Structural Anomalies and Repair
- Congenital Heart Disease Studies
- Reconstructive Surgery and Microvascular Techniques
- Cellular transport and secretion
- Burn Injury Management and Outcomes
- Fibroblast Growth Factor Research
- Infectious Aortic and Vascular Conditions
- Corneal Surgery and Treatments
- Myasthenia Gravis and Thymoma
- Surgical Sutures and Adhesives
- Metabolism and Genetic Disorders
- Extracellular vesicles in disease
- Retinal Development and Disorders
- Bone Tissue Engineering Materials
- Intraocular Surgery and Lenses
- Tendon Structure and Treatment
- Breast Implant and Reconstruction
- Parkinson's Disease and Spinal Disorders
- Cancer Treatment and Pharmacology
- Oral and gingival health research
Nationwide Children's Hospital
2019-2024
Abbott (United States)
2024
The Ohio State University
2018-2022
Shriners Hospitals for Children - Cincinnati
2018-2019
University of Cincinnati
2019
University of Cincinnati Medical Center
2019
Solothurner Spitäler
1960
We developed a tissue-engineered vascular graft (TEVG) for use in children and present results of U.S. Food Drug Administration (FDA)-approved clinical trial evaluating this patients with single-ventricle cardiac anomalies. The TEVG was used as Fontan conduit to connect the inferior vena cava pulmonary artery, but high incidence narrowing manifested within first 6 months, which treated successfully angioplasty. To elucidate mechanisms underlying early stenosis, we data-informed,...
Abstract Background Tissue-engineered vascular grafts (TEVGs) have the potential to advance surgical management of infants and children requiring congenital heart surgery by creating functional conduits with growth capacity. Methods Herein, we used an integrative computational-experimental approach elucidate natural history neovessel formation in a large animal preclinical model; combining vitro accelerated degradation study mechanical testing, implantation studies vivo imaging histology,...
In the field of congenital heart surgery, tissue-engineered vascular grafts (TEVGs) are a promising alternative to traditionally used synthetic grafts. Our group has pioneered use TEVGs as conduit between inferior vena cava and pulmonary arteries in Fontan operation. The natural history graft remodeling its effect on hemodynamic performance not been well characterized. this study, we provide detailed analysis first U.S. clinical trial evaluating treatment disease. We show two distinct phases...
Native arteries contain a distinctive intima-media composed of organized elastin and an adventitia containing mature collagen fibrils. In contrast, implanted biodegradable small-diameter vascular grafts do not present spatially regenerated, elastin. The elastin-containing structures within the region encompass elastic lamellae (EL) internal lamina (IEL) are crucial for normal arterial function. Here, development novel electrospun graft that facilitates de novo formation structurally...
Lysosomes and lysosome related organelles (LROs) are dynamic at the intersection of various pathways involved in maintaining cellular hemostasis regulating functions. Vesicle trafficking lysosomes LROs critical to maintain their The lysosomal regulator (LYST) is an elusive protein important for regulation membrane dynamics intracellular LROs. Mutations LYST gene result Chédiak-Higashi syndrome, autosomal recessive immunodeficiency characterized by defective granule exocytosis, cytotoxicity,...
Abstract Tissue engineered vascular grafts (TEVGs) using scaffolds fabricated from braided poly(glycolic acid) (PGA) fibers coated with poly(glycerol sebacate) (PGS) are developed. The approach relies on in vivo tissue engineering by which neotissue forms solely within the body after a scaffold has been implanted. Herein, impact of altering braid design and coating formation is investigated. Several combinations braiding parameters manufactured evaluated Beige mouse model infrarenal...
Pressure garment therapy is often prescribed to improve scar properties following full-thickness burn injuries. generally recommended for long periods of time injury (1–2 years), though it plagued by extremely low patient compliance. The goal this study was examine the effects early cessation pressure on properties. Full-thickness injuries were created along dorsum red Duroc pigs. eschar excised and wound sites autografted with split-thickness skin. Scars treated garments within 1 week...
Pressure garment therapy, used for reduction of postburn scarring, is commonly initiated after complete healing the wound or autograft. Although some clinicians have suggested that earlier treatment may improve outcomes, effect early initiation therapy has not been studied in a controlled environment.Full-thickness burns were created on red Duroc pigs, burn eschar was excised, and bed grafted with split-thickness autografts. Grafts treated pressure garments immediately, 1 week (early), 5...
Abstract Tissue engineered vascular grafts hold promise for the creation of functional blood vessels from biodegradable scaffolds. Because precise mechanisms regulating this process are still under investigation, inducible genetic mouse models an important and widely used research tool. However, here we describe importance challenging baseline assumption that tamoxifen is inert when as a small molecule inducer in context cardiovascular tissue engineering. Employing standard inferior vena...
Objective: Despite the development of a number treatment modalities, scarring remains common postburn injury. To reduce burn scarring, pressure garment therapy has been widely utilized but is complicated by low patient adherence. improve adherence, reduced hours daily wear proposed. Approach: examine efficacy at durations wear, porcine burn-excise-autograft model was utilized. Grafted burns were treated with garments (20 mmHg) for 8, 16, or 24 h untreated serving as controls. Scar area,...
Wall stress is often lower in tissue-engineered constructs than comparable native tissues due to the use of stiff polymeric materials having thicker walls. In this work, we sought design a murine arterial graft more favorable local mechanical environment for infiltrating cells; used electrospinning enclose compliant inner core poly(glycerol sebacate) with stiffer sheath poly(caprolactone) reduce potential rupture. Two scaffolds were designed that differed thickness as previous computational...
Background Ventricular septal perforation and left ventricular aneurysm are examples of potentially fatal complications myocardial infarction. While various artificial materials used in the repair these issues, possibility associated infection calcification is non-negligible. Cell-seeded biodegradable tissue-engineered patches may be a potential solution. This study evaluated feasibility new patch rat model to neotissue formation cardiac patches. Methods Human induced pluripotent stem...
Abstract Tissue engineered vascular grafts (TEVGs) are a promising technology, but hindered by occlusion. Seeding with bone‐marrow derived mononuclear cells (BM‐MNCs) mitigates occlusion, yet the precise mechanism remains unclear. Seeded disappear quickly and potentially mediate an anti‐inflammatory effect through paracrine signaling. Here, series of reciprocal genetic TEVG implantations plus recombinant protein treatment is reported to investigate what role interleukin‐10, cytokine, plays...
Abstract Non‐healing wounds are a major threat to public health throughout the United States. Tissue healing is complex multifactorial process that requires synchronicity of several cell types. Endolysosomal trafficking, which contributes various functions from protein degradation plasma membrane repair, an understudied in context wound healing. The lysosomal trafficking regulator (LYST) essential endolysosomal system through indeterminate mechanism. In this study, we examine impact impaired...
Capsular contracture as a result of the foreign body response (FBR) is common issue after implant-based breast reconstruction, affecting up to 20% patients. New evidence suggests that tamoxifen may mitigate FBR. C57BL/6 female mice were treated with daily or control injections and implanted bilateral silicone implants in submammary glandular plane. Implants removed
Macrophages are a critical driver of neovessel formation in tissue-engineered vascular grafts (TEVGs), but also contribute to graft stenosis, leading clinical trial complication. Macrophage depletion via liposomal delivery clodronate, first-generation bisphosphonate, mitigates simultaneously leads complete lack tissue development TEVGs. This result and the associated difficulty utilizing means that clodronate may not be an ideal preventing stenosis. Newer generation bisphosphonates, such as...
In article number 2001093 by Jacob C. Zbinden, Christopher K. Breuer, and co-workers, tissue engineered arterial grafts are created from braided poly(glycolic acid) fibers at the boundaries of manufacturable design space with without a poly(glycerol sebacate) coating evaluated in mouse intrarenal aortic model. Implants followed 4D ultrasound explants biaxial mechanical testing histological analysis.