A5024894629- Phosphodiesterase function and regulation
- Cholinesterase and Neurodegenerative Diseases
- Chemical synthesis and alkaloids
- Advancements in Transdermal Drug Delivery
- RNA Interference and Gene Delivery
- 3D Printing in Biomedical Research
- Nerve injury and regeneration
- Advanced Fluorescence Microscopy Techniques
- Advanced biosensing and bioanalysis techniques
- Osteoarthritis Treatment and Mechanisms
- Neuroscience and Neural Engineering
- Synthesis and Catalytic Reactions
- Electrospun Nanofibers in Biomedical Applications
- Bone Tissue Engineering Materials
- Advanced Drug Delivery Systems
- Optical measurement and interference techniques
- Pulmonary Hypertension Research and Treatments
- Spinal Fractures and Fixation Techniques
- Knee injuries and reconstruction techniques
- Spine and Intervertebral Disc Pathology
- Advanced X-ray Imaging Techniques
- Digital Holography and Microscopy
- X-ray Diffraction in Crystallography
- Nanoparticle-Based Drug Delivery
- SARS-CoV-2 and COVID-19 Research
Hainan University
2021-2025
National Taiwan University
2013-2024
National Taiwan University Hospital
2006-2023
Sun Yat-sen University
2013-2022
University of North Carolina at Chapel Hill
2018
UNC Lineberger Comprehensive Cancer Center
2018
National Taiwan Ocean University
2011
Taipei Medical University
2009
Abstract Metachronous liver metastases (MLM) are characterised by high incidence and mortality in clinical colorectal cancer treatment. Currently traditional methods cannot effectively predict prevent the occurrence of metachronous metastasis cancer. Based on 5hmC‐Seal analysis blood tissue samples, this study found that portal venous was more relevant to tumour gDNA than peripheral blood. We performed a novel epigenetic liquid biopsy strategy using 10 5hmC alterations, accurately...
Reconstruction of a segmental fracture with massive bone loss is still challenge for orthopaedic surgeons. The aim our study was to develop suitable biodegradable thermosensitive hydrogel system as carrier morphogenetic protein (BMP)-2 delivery in the treatment critical-sized femoral defects. A block copolymer composed monomethoxypoly(ethylene glycol) (mPEG), poly(lactic-co-glycolic acid) (PLGA) and 2, 2'-Bis (2-oxazolin) (Box) synthesized by ring opening polymerization. characterized 1H-NMR...
Autologous chondrocyte implantation (ACI) has been recently used to treat cartilage defects. Partly because of the success mosaicplasty, a procedure that involves native osteochondral plugs, it is potential significance consider application ACI in form biphasic composites. To test clinical applicability such composite construct, we repaired defect with at low cell-seeding density on scaffold, and combined graft harvest single surgery. We fabricated cylindrical porous plug...
We performed in vivo THz transmission imaging study on a subcutaneous xenograft mouse model for early human breast cancer detection. With THz-fiber-scanning system, we continuously monitored the growth of mice. Our not only indicates that can distinguish from surrounding fatty tissue, but also with high sensitivity. will encourage broad and further investigations future screening by using system.
Selaginpulvilins A–D (1–4), four new phenols with an unprecedented 9,9-diphenyl-1-(phenylethynyl)-9H-fluorene skeleton, together known selaginellins (5–8) were isolated from Selaginella pulvinata. Their structures elucidated by spectroscopic analysis and chemical correlation. The structure of 1 was confirmed single-crystal X-ray diffraction. Compounds 1–8 exhibited remarkable inhibitory activities (IC50 values in the range 0.11–5.13 μM) against phosphodiesterase-4 (PDE4), a drug target for...
Bioassay-guided fractionation of the ethanolic extract roots Toddalia asiatica led to isolation seven new prenylated coumarins (1–7) and 14 known analogues (8–21). The structures 1–7 were elucidated by spectroscopic analysis, their absolute configurations determined combined chemical methods chiral separation analysis. Compounds 1–5, named toddalin A, 3‴-O-demethyltoddalin toddalins B–D, represent an unusual group phenylpropenoic acid-coupled coumarins. 1–21 four modified analogues, 10a,...
Accurate prediction of absolute protein–ligand binding free energy could considerably enhance the success rate structure-based drug design but is extremely challenging and time-consuming. Free perturbation (FEP) has been proven reliable limited to relative energies similar ligands (with only minor structural differences) in with a same target practical applications. Herein, Gaussian algorithm-enhanced FEP (GA-FEP) protocol developed simulation performance, enabling efficiently carry out...
Abstract The human coronavirus HCoV-19 infection can cause acute respiratory distress syndrome (ARDS), hypercoagulability, hypertension, extrapulmonary multiorgan dysfunction. Effective antiviral and anti-coagulation agents with safe clinical profiles are urgently needed to improve the overall prognosis. We screened an FDA approved drug library found that anticoagulant agent dipyridamole (DIP) suppressed replication at EC50 of 100 nM in vitro . It also elicited potent type I interferon...
Psoriasis is a common immune-mediated skin disorder manifesting in abnormal plaques, and phosphodiesterase 4 (PDE4) an effective target for the treatment of inflammatory diseases such as psoriasis. Toddacoumalone natural PDE4 inhibitor with moderate potency imperfect drug-like properties. To discover novel potent inhibitors considerable druggability, series toddacoumalone derivatives were designed synthesized, leading to compound...
Attachment to and proliferation on the substrate are deemed important considerations when Schwann cells (SCs) be seeded in synthetic nerve grafts. Good attachment is a prerequisite for SCs survive. Fast will yield large numbers of short time, which appears promising stimulating peripheral regeneration. However, surface properties dominating factor influencing interactions between The aim this study was investigate effects laminin modified PLGA chitosan membranes after chemical method plasma...
Abstract Adhesions are unavoidable consequences of surgery and other trauma. How to prevent the adhesions remains a big issue in healthcare system. The objective this study is test efficacy polycaprolactone (PCL) films as physical barriers reducing postoperative intra‐abdominal rat cecum‐abdominal wall model. PCL quite cheap compared with agents recently used market. fabrication method also very easy perform. Scanning electron microscope (SEM) showed multiple pores over upper bottom surfaces...
Licochalcone B (LCB), an extract from the root of Glycyrrhiza inflate, has same caffeic acid scaffold as curcumin (Cur), which is known anti-Alzheimer's disease (AD) agent. However, there no relevant research about anti-AD activity LCB. In this study, LCB was investigated. could inhibit amyloid beta (Aβ42) self-aggregation (IC50 = 2.16 ± 0.24 μM) and disaggregate pre-formed Aβ42 fibrils, reduce metal-induced aggregation through chelating metal ions. Molecular docking further revealed that...
Pulmonary arterial hypertension (PAH) causes pathological increase in pulmonary vascular resistance, leading to right-heart failure and eventual death. Previously, phosphodiesterase-10 (PDE10) was reported be a promising target for PAH based on the studies with nonselective PDE inhibitor papaverine, but little progress has been made confirm practical application of PDE10 inhibitors. To validate whether is ameliorated by inhibition rather than other isoforms, here we report an integrated...
Abstract Coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a global crisis. There is no therapeutic treatment specific for COVID-19. It highly desirable to identify potential antiviral agents against SARS-CoV-2 from existing drugs available other diseases and, thus, repurpose them of In general, drug repurposing effort new disease, such as COVID-19, usually starts virtual screening drugs, followed experimental...
Articular cartilage is known to have poor healing capacity after injury. Autologous chondral grafting remains the mainstay treat well-defined, full-thickness, symptomatic defects. We demonstrated utilization of gelatin microbeads deliver autologous chondrocytes for in vivo generation. Chondrocytes were harvested from left forelimbs 12 Lee-Sung pigs. The cells expanded monolayer culture and then seeded onto or monolayer. Shortly before implantation, cell-laden beads mixed with collagen type I...
Abstract Nonviral gene carriers composed of biodegradable polymers or lipids have been considered as a safer alternative for over viral vectors. We developed multifunctional nanomicelles both drug and delivery application. Polyethylenimine (PEI) was modified by grafting stearic acid (SA) further formulated to polymeric micelles (PEI‐SA) with positive surface charge evaluation. Our results showed that PEI‐SA provided high siRNA binding efficiency exhibited low cytotoxicity compared unmodified...
Phosphodiesterase-2A (PDE2A) is a potential therapeutic target for treatment of Alzheimer's disease and pulmonary hypertension. However, most the current PDE2A inhibitors have moderate selectivity over other PDEs. In present study, we described discovery novel by structure-based virtual screening combining pharmacophore model screening, molecular docking, dynamics simulations, bioassay validation. Nine hits out 30 molecules from SPECS database (a hit rate 30%) inhibited with affinity less...
Reporter proteins have broad applications in visualizing molecular events at the cellular, tissue and whole-body levels. Transmembrane transporters recognizing specific domains are of particular interest because they enable migration signal-source molecules from extracellular space to cytoplasm for subsequent application multimodality imaging. Organic anion-transporting polypeptides (OATPs) demonstrated their MRI reporter efficacy. We further expanded use as a dual-modality noninvasive vivo...