A5101444231- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- SARS-CoV-2 and COVID-19 Research
- Bacteriophages and microbial interactions
- Immunotherapy and Immune Responses
- Antimicrobial Peptides and Activities
- Immune Response and Inflammation
- Peptidase Inhibition and Analysis
- Global Health Care Issues
- Climate Change and Health Impacts
- Air Quality and Health Impacts
- COVID-19 Clinical Research Studies
- Antibiotic Resistance in Bacteria
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Advanced Fluorescence Microscopy Techniques
- Nanopore and Nanochannel Transport Studies
- COVID-19 epidemiological studies
- Optical Coherence Tomography Applications
- Carbohydrate Chemistry and Synthesis
- HIV Research and Treatment
- Photoacoustic and Ultrasonic Imaging
- Lipid Membrane Structure and Behavior
- Venomous Animal Envenomation and Studies
Chinese Academy of Sciences
1996-2025
Xinjiang Institute of Ecology and Geography
2023-2025
University of Chinese Academy of Sciences
2019-2025
Third Affiliated Hospital of Zhengzhou University
2025
Institute for Social and Economic Research
2020-2024
Boston Children's Hospital
2023-2024
Beijing Children’s Hospital
2024
Capital Medical University
2024
Shanghai Institute of Materia Medica
2023-2024
Jinan University
2020-2024
This study models local and cross-city transmissions of the novel coronavirus in China between January 19 February 29, 2020. We examine role various socioeconomic mediating factors, including public health measures that encourage social distancing communities. Weather characteristics 2 weeks prior are used as instrumental variables for causal inference. Stringent quarantines, city lockdowns, imposed late significantly decreased virus transmission rate. The spread was contained by middle...
Coronavirus 3′-to-5′ exoribonuclease (ExoN), residing in the nonstructural protein (nsp) 10–nsp14 complex, boosts replication fidelity by proofreading RNA synthesis and is critical for virus life cycle. ExoN also recognizes excises nucleotide analog inhibitors incorporated into nascent RNA, undermining effectiveness of analog–based antivirals. Here we present cryo–electron microscopy structures both wild-type mutant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nsp10-nsp14...
The chromosomally encoded arsenical resistance (ars) operon subcloned into a multicopy plasmid was found to confer moderate level of arsenite and antimonite in Escherichia coli. When the deleted from chromosome, cells exhibited hypersensitivity arsenite, antimonite, arsenate. Expression ars genes inducible by arsenite. By Southern hybridization, all strains E. coli examined but not Salmonella typhimurium, Pseudomonas aeruginosa, or Bacillus subtilis.
Abstract This paper examines the role of various socioeconomic factors in mediating local and cross-city transmissions novel coronavirus 2019 (COVID-19) China. We implement a machine learning approach to select instrumental variables that strongly predict virus transmission among rich exogenous weather characteristics. Our 2SLS estimates show stringent quarantine, massive lockdown other public health measures imposed late January significantly reduced rate COVID-19. By early February, spread...
Xanthomonas oryzae pv. is a destructive bacterial disease of rice, and the development an environmentally safe bactericide urgently needed. Antimicrobial peptides, as antibacterial sources, may play important roles in development. In present study, we found that antimicrobial peptide melittin had desired activity against X. oryzae. The mechanism was investigated by examining its effects on cell membranes, energy metabolism, nucleic acid, protein synthesis. arose from ability to interact with...
The transcription of the ars operon is negatively controlled by ArsR repressor and induced arsenite antimonite.Using hydroxylamine mutagenesis, four arsR mutants were isolated; three selected for inability to respond inducers: C32Y, C32F, C34Y.Each altered proteins still bound specifically operator, but inducers less effective in effecting release from DNA.Each mutant genes trans with a reporter gene promoter repressed expression gene, addition inducer did not relieve repression.These...
The DnaK, DnaJ, and GrpE heat shock proteins are required for motility of Escherichia coli. Cells deleted dnaK or dnaJ, with some mutations in the grpE gene, nonmotile, lack flagella, exhibit a 10- to 20-fold decrease rate synthesis flagellin, show reduced rates transcription both flhD master operon (encoding FlhD FlhC) fliA sigma F). Genetic studies suggest that DnaK DnaJ define regulatory pathway affecting is independent cyclic AMP-catabolite gene activator protein chemotaxis system.
Trans-translation mediated by SsrA (tmRNA) and its associated protein SmpB plays an important role in rescuing stalled ribosomes detoxifying toxic products under stress conditions. However, the of bacterial persister survival has not been studied. The recent finding that pyrazinamide as a unique drug inhibits trans-translation Mycobacterium tuberculosis prompted us to examine survival. Using Escherichia coli model, we constructed mutants assessed susceptibility various antibiotics conditions...
ABSTRACT This study was designed to evaluate the immunogenicity and protective efficacy of a divalent fusion DNA vaccine encoding both Brucella abortus L7/L12 protein (ribosomal protein) Omp16 (outer membrane lipoprotein), designated pcDNA3.1-L7/L12-Omp16. Intramuscular injection this into BALB/c mice elicited markedly humoral cellular immune responses. The specific antibodies exhibited dominance immunoglobulin G2a (IgG2a) over IgG1. In addition, dual-gene strong T-cell proliferative...
For over 30 years, HIV/AIDS has wreaked havoc in the world. In absence of an effective vaccine for HIV, development new anti-HIV agents is urgently needed. We previously identified antiviral activities scorpion-venom-peptide-derived mucroporin-M1 three RNA viruses (measles viruses, SARS-CoV, and H5N1). this investigation, a panel scorpion venom peptides their derivatives were designed chosen assessment activities. A peptide derivative Kn2-7 was as most potent anti-HIV-1 by screening assays...
Breast cancer is a prevalent malignancy affecting women globally, characterized by significant morbidity and mortality rates. Ecliptae Herba traditional herbal medicine commonly used in clinical practice, has recently been found to possess antitumor properties. In order explore the underlying material basis molecular mechanisms responsible for anti-breast effects of Herba, we network pharmacology experimental verification. UPLC-MS/MS was utilized identify compounds present Herba. The active...
Escherichia coli lacks flagella when grown in tryptone broth the presence of various adverse conditions (C. Li, C. J. Louise, W. Shi, and Adler, Bacteriol. 175:2229-2235, 1993). Now, synthesis, rather than degradation, flagellin was shown to be inhibited. Studies transcriptional fusions flagellar operons lacZ gene revealed that transcription genes reduced cells under these conditions. Increasing dosage flhD operon by a plasmid partially suppressed nonflagellation caused some The signal which...
ObjectivesMycobacterial persistence is thought to be the underlying cause of current lengthy tuberculosis therapy and latent infection. Despite some recent progress, mechanisms bacterial are poorly understood. We have recently identified a new persister gene phoU from Escherichia coli shown that mutant has defect in persisters. The objective this study evaluate role two homologues phoY1 phoY2 Mycobacterium mycobacterial persistence.
Transcription activation by cyclic AMP (cAMP) receptor protein (CAP) is the classic paradigm of transcription regulation in bacteria. CAP was suggested to activate on class-II promoters via a recruitment and isomerization mechanism. However, whether how it modifies RNA polymerase (RNAP) initiate remains unclear. Here, we report cryo–electron microscopy (cryo-EM) structures an intact Escherichia coli CAP-dependent complex (CAP-TAC) with without de novo transcript. The reveal two distinct...
Abstract Bacterial RNA polymerase (RNAP) holoenzyme initiates transcription by recognizing the conserved –35 and –10 promoter elements that are optimally separated a 17-bp spacer. The MerR family of transcriptional regulators activate suboptimal 19–20 bp spacer promoters in response to myriad cellular signals, ranging from heavy metals drug-like compounds. regulation is not fully understood. Here we report one crystal structure multidrug-sensing regulator EcmrR nine cryo-electron microscopy...
Abstract Carbohydrates play pivotal roles in an array of essential biological processes and are consequently involved many diseases. To meet the needs glycobiology research, chemical enzymatic non‐enzymatic methods have been developed to generate glycoconjugates with well‐defined structures. Herein, harnessing unique properties C6‐oxidized glycans, we report a straightforward robust strategy for site‐ stereoselective glycomodification biomolecules N‐terminal tryptophan residues by...
A human papillomavirus type 16 E7 DNA vaccine with the open reading frame encoding mutations in two zinc-binding motifs expressed a rapidly degraded protein. This induced significantly stronger E7-specific cytotoxic T-lymphocyte response and better tumor protection mice than did wild-type expressing stable
We previously demonstrated that an anti-caries DNA vaccine intranasally administered with recombinant flagellin protein as a mucosal adjuvant enhanced salivary IgA response and conferred better protection against caries. However, the relatively weak immunogenicity of vaccines necessity for large quantity antigens remain significant challenges. Here, we fused derived from E. coli (KF) target antigen PAc containing A-P fragment S. mutans (rPAc) to produce single (KF-rPAc). The abilities...