Abstract Objective This study was undertaken to investigate the effect of lymphatic inhibition on joint and draining lymph node (LN) pathology during course arthritis progression in mice. Methods Tumor necrosis factor (TNF)–transgenic mice were used as a model chronic inflammatory arthritis. Mice subjected contrast‐enhanced magnetic resonance imaging obtain ankle knee synovial volumes popliteal LN before after 8 weeks treatment with vascular endothelial growth receptor 3 (VEGFR‐3)...

Abstract Objective To investigate whether the enhancement of joint lymphangiogenesis by injection vascular endothelial growth factor C (VEGF‐C) adeno‐associated virus (AAV) into affected joints has therapeutic efficacy in chronic inflammatory arthritis mice. Methods Tumor necrosis factor–transgenic (TNF‐Tg) mice were used as a model arthritis. Human VEGF‐C was cloned an AAV expression vector to generate AAV‐VEGF‐C. The TNF‐Tg injected with AAV‐VEGF‐C or AAV‐luciferase (AAV‐Luc) control....

Chronic inflammatory disorders, such as rheumatoid arthritis, are often accompanied by systemic bone loss, which is thought to occur through cytokine-mediated stimulation of osteoclast resorption and inhibition osteoblast function. However, the mechanisms involved in remain poorly understood. Here we test hypothesis that increased Smad ubiquitin regulatory factor 1 (Smurf1)-mediated degradation morphogenetic protein pathway signaling proteins mediates reduced formation disorders. Osteoblasts...

Abstract Osteoporosis is defined as reduced bone mineral density with a high risk of fragile fracture. Current available treatment regimens include antiresorptive drugs such estrogen receptor analogues and bisphosphates anabolic agents parathyroid hormone (PTH). However, neither option completely satisfactory because adverse effects. It thus highly desirable to identify novel improve future osteoporosis treatment. Osthole, coumarin-like derivative extracted from Chinese herbs, has been shown...

To develop an in vivo imaging method to assess lymphatic draining function the K/BxN mouse model of inflammatory arthritis.Indocyanine green, a near-infrared fluorescent dye, was injected intradermally into footpads wild-type mice, limbs were illuminated with 806-nm laser, and movement indocyanine green from injection site popliteal lymph node (LN) recorded CCD camera. Indocyanine images analyzed obtain 5 measures across time. Images arthritic mice control nonarthritic littermates obtained...

Osteoclasts are bone-resorbing cells, but they also secrete and respond to cytokines. Here, we test the hypothesis that osteoclasts lymphatic growth factor, VEGF-C, increase their resorptive activity. osteoclast precursors were generated by culturing splenocytes with macrophage colony-stimulating factor RANKL from wild-type, NF-kappaBp50(-/-)/p52(-/-), Src(-/-) mice. Expression of VEGFs was measured real time reverse transcription-PCR, Western blotting, immunostaining. The effect VEGF-C...

The potential of increasing bone mass and preventing fractures in osteoporosis using stem cell therapy is currently an area intense focus. However, there are very little data available regarding the postfracture bony defect healing efficacy under osteoporotic conditions. This study aims to investigate whether critical-sized segmental defects a rabbit model could be repaired allogenic cell-based tissue engineering (TE) approach influence on treatment efficacy. Rabbit fetal marrow mesenchymal...

Establishment of a novel in vivo animal model cervical spondylosis.To investigate apoptotic, degenerative, and inflammatory changes occurring the intervertebral discs rats.Cervical degeneration occurs as result imbalance both static dynamic spinal stabilizers. The disc that is characterized by increased local inflammation apoptosis cells.By excising paraspinal musculature posterior ligaments rats, stabilizers were disrupted. resultant biomechanical resulted biochemical histologic changes,...

Abstract Introduction Rheumatoid arthritis (RA) is a chronic autoimmune disease with episodic flares in affected joints. However, how arthritic flare occurs only select joints during systemic remains an enigma. To better understand these observations, we developed longitudinal imaging outcomes of synovitis and lymphatic flow mouse models RA, identified that asymmetric knee associated ipsilateral popliteal lymph node (PLN) collapse the translocation CD23 + /CD21 hi B-cells (B-in) into...

In Brief Objective Oleanolic acid (OA) and its glycosides have been reported to prevent bone loss by inhibiting the formation of osteoclasts. However, because resorption are balanced processes in metabolism, no studies described effect OA on osteogenesis. The aim present study was evaluate osteoprotective rats with ovariectomy (OVX)-induced osteoporosis search for molecular targets mesenchymal stem cells (bMSCs). Methods Two-month-old female mice that underwent OVX were treated (20 mg/kg a...

Both forelimbs of rats were amputated, and these kept in the custom-made cages that prolonged upright posture. Pathologic changes observed lumbar spine at different time points after surgery.To investigate effect posture on intervertebral discs rat spine.Previous studies have shown increased axial forces can decrease height disc, but there are no data to indicate whether or not long-term repeated assumption could result degenerative changes.The 30 amputated when they 1-month old. These...

Tissue engineered bone grafts (TEBG) using mesenchymal stem cells (MSCs) demonstrate great potential for defect treatment.

Most chronic neck pain is the result of degeneration cervical spine. IL-1beta may play an important role in intervertebral disc degeneration. This being case, inhibiting could provide a therapeutic approach for reducing or preventing Muscone reportedly relieves and suppresses inflammation. Therefore, we asked whether muscone, potent antiinflammatory agent, reduce proinflammatory cytokines vitro (end-plate cartilage cultures) end-plate vivo (a rat model that induces degeneration). In vitro,...

Three-dimensional quantitative structure-activity relationship (3D-QSAR) serves as one of the most important and effective tools to guide molecular design for development new pesticides. According principle structural splicing, only changing a small group may lead great increase in activity while maintaining active center unchanged. Under guidance 3D-QSAR, three series phenylpyrazole oxime fluorescent insecticides acting on GABA receptor, namely, esters (POEs), ethers (POETs), triazoles...

In Brief Study Design. Pathologic changes were observed in the spine of small mother against decapentaplegic (Smad) 3−/− mice at different time points. Objective. To observe degeneration intervertebral disc (IVD) Smad3 gene knock-out with growth. Summary Background Data. (Smad3−/−) displays phenotypes similar to human osteoarthritis. Despite similarities between IVD cartilage endplate and articular cartilage, there has been relatively little interest exploring possibility that might be...

To investigate whether Du‐Huo‐Ji‐Sheng‐Tang (DHJST) attenuate inflammation of RA related to lymphatic drainage function in vivo, we treated eight 3‐month‐old TNF‐Tg mice with DHJST (12 g/kg) or the same volume physiological saline once every day for 12 weeks, and WT littermates were used as negative control. After twelve performed NIR‐ICG imaging found that increased ICG clearance at footpad pulse efferent vessel between popliteal lymph node footpad. Histology staining ankle joints showed...

Aims: Prostate cancer is a well-known aggressive malignant tumor in men with high metastasis rate and poor prognosis. Adapalene (ADA) third-generation synthetic retinoid anticancer properties. We investigated the anti-tumor activity molecular mechanisms of ADA RM-1 prostate cell line vivo vitro. Methods: The effects on proliferation were estimated using CCK-8 colony formation assays. wound-healing assay Transwell employed to examine migratory capacity invasiveness cells. Flow cytometry was...

Osteoblasts are the main functional cells of bone formation, and they responsible for synthesis, secretion, mineralization matrix. Phosphatidylinositol-3-kinase/Akt is an important signaling pathway involved in regulation cell proliferation, death, survival. Some studies have shown that 3-phosphoinositide-dependent protein kinase-1 (PDK-1) plays role phosphorylation Akt. In present study, osteocalcin (OCN) promoter-driven Cre-LoxP system was established to specifically delete PDK-1 gene...

Perturbations in the balanced process of osteoblast-mediated bone formation and osteoclast-mediated resorption leading to excessive osteoclast and/or activity is cause many pathological conditions such as osteoporosis. The only cell body capable resorbing degrading mineralized matrix. Osteoclast from monocytic precursors governed by actions two key cytokines macrophage-colony-stimulating factor receptor activator nuclear factor-κB ligand (RANKL). Binding RANKL binding RANK initiates a series...