Abstract Extravasated erythrocytes in cerebrospinal fluid (CSF) critically contribute to the pathogenesis of subarachnoid hemorrhage (SAH). Meningeal lymphatics have been reported drain macromolecules and immune cells from CSF into cervical lymph nodes (CLNs). However, whether meningeal are involved clearing extravasated after SAH remains unclear. Here we show that a markedly higher number accumulated CLNs SAH. When depleted mouse model SAH, degree erythrocyte aggregation is significantly...

Osteoarthritis (OA) is a common chronic inflammatory disorder. Effective remodeling of microenvironment in the joint promising strategy to prevent OA. However, current drugs remain unsatisfactory due lack targeted and effective ways for relieving conditions OA joints. Bortezomib (BTZ), proteasome inhibitor, could effectively inhibit proinflammatory cytokines but with poor accumulation tissues. To overcome shortcomings BTZ delivery improve efficacy therapy, herein, we designed novel...

The risk of developing osteoporosis (OP) is increased in patients with rheumatoid arthritis (RA), which associated poorer prognosis and higher mortality. Many RA may experience bone loss early the disease course. Therefore, timely assessment OP essential. This a retrospective study we collected information from 500 who underwent mineral density assessments at Longhua Hospital, Shanghai University Traditional Chinese Medicine, January 2018 to December 2022. Based on data collection timeline,...

Astragalin, as a bioactive flavonoid with anti-inflammatory, antioxidant, and protective properties, provides potential agent for rheumatoid arthritis (RA). In this study, its therapeutic efficacy the underlying mechanisms were explored using DBA/1J mice collagen-induced (CIA). It was demonstrated that astragalin could significantly attenuate inflammation of CIA mice. The effects associated decreased severity (based on index), joint swelling reduced bone erosion destruction. Furthermore,...

To investigate the distribution and alteration of lymphatic vessels draining function in knee joints normal osteoarthritic mice.For mouse models osteoarthritis (OA), we used mice with meniscal-ligamentous injury or conditional knockout gene for cartilage transforming growth factor β (TGFβ) type II receptor. The severity loss joint destruction was assessed histologically. Capillary mature were identified analyzed using double immunofluorescence staining a whole-slide digital imaging system....

In this study, we sought to determine the cellular source of inducible nitric oxide synthase (iNOS) induced in lymphatic endothelial cells (LECs) response tumor necrosis factor (TNF), effects iNOS on smooth muscle cell (LSMC) function and development arthritis TNF-transgenic (TNF-Tg) mice, whether inhibitors improve reduce joint destruction inflammatory erosive arthritis. We used quantitative polymerase chain reactions, immunohistochemistry, histology, near-infrared imaging examine (1)...

Abstract: Triptolide (TP) exhibits immunosuppressive, cartilage-protective and anti-inflammatory effects in rheumatoid arthritis. However, the toxicity of TP limits its widespread use. To decrease toxic effects, we developed a novel nano-drug carrier system containing using poly-γ-glutamic acid-grafted di-tert-butyl L-aspartate hydrochloride (PAT). PAT had an average diameter 79±18 nm, narrow polydispersity index (0.18), strong zeta potential (-32 mV) high drug encapsulation efficiency (EE 1...

The musculoskeletal system contains an extensive network of lymphatic vessels. Decreased lymph flow the draining collecting lymphatics usually occurs in clinic after traumatic fractures. However, whether defects drainage can affect fracture healing is unclear. To investigate effect dysfunction on healing, we used a selective VEGFR3 tyrosine kinase inhibitor to treat tibial fractured mice for 5 weeks versus vehicle-treated control. ensure successfully establishing deceased model mice,...

This study aimed to investigate the effects of bile acids (BAs) supplementation on fatty liver hemorrhagic syndrome (FLHS), production performance, and physiological quality characteristics laying hen eggs. Sixty Sanhuang hens, aged 28 weeks, were randomly allocated six dietary treatments over a 4-week period, including control (CON) group (feeding basal diet), high-fat diet (HFD)-treated (basal containing 10% soybean oil), HFD supplemented with 0.01% 0.02% chenodeoxycholic acid (CDCA) or...

ABSTRACT Background Hydrocephalus is characterized by secretion, circulation, and absorption disorder of cerebrospinal fluid (CSF) with high morbidity complication rate. The relationship between inflammation abnormal secretion CSF choroid plexus epithelium (CPE) had received more attention. In this study, we aim to detect the role Toll‐like receptor 4/nuclear factor‐kappa B/Na+/K+/2Cl‐cotransporter 1(TLR4/NF‐κB/NKCC1) signal pathway in development hydrocephalus. Method was induced adult rats...

Abstract: Triptolide (TP) displays a strong immunosuppression function in immune-mediated diseases, especially the treatment of rheumatoid arthritis. However, addition to its medical and health-related functions, TP also exhibits diverse pharmacological side effects, for instance, liver kidney toxicity myelosuppression. In order reduce nano drug carrier system (γ-PGA-l-PAE-TP [PPT]), which was loaded by poly-γ-glutamic acid-grafted l-phenylalanine ethylester copolymer, developed. PPT...

To investigate the roles of synovial lymphatic system in severity and progression joint tissue damage functional responses endothelial cells (LECs) to macrophage subsets, evaluate therapeutic potential proteasome inhibitor bortezomib (BTZ) a mouse model experimental posttraumatic osteoarthritis (OA).C57BL/6J wild-type mice received meniscal ligamentous injury induce knee OA. Lymphangiogenesis was blocked by vascular growth factor receptor 3 (VEGFR-3) neutralizing antibody. Synovial drainage...

Rheumatoid arthritis (RA) is a chronic autoimmune disease that primarily characterized by synovial inflammation. Our previous studies demonstrated the lymphatic system critical for development and maintenance of RA disease, sufficient lymph drainage helps to improve joint In this study, we found NG-R1, main active component in traditional Chinese medicinal herb Sanchi, activating function can attenuate According histopathological staining ankle sections, NG-R1 significantly decreased area...

Background: Huangqi Guizhi Wuwu Decoction (HGWD) is a traditional and effective Chinese medicine compound decoction for the treatment of rheumatoid arthritis (RA). However, there few research on cardiopulmonary complications. The present study was to whether HGWD can alleviate pathological changes caused by Methods: Five 3-month-old TNF-Tg mice were treated with (9.1 g/kg) once day or same dose normal saline lasted 8 weeks, wild-type littermates age used as negative control, methotrexate...

The study is aimed to determine the effects of cynarin (Cyn) on mice with gouty arthritis (GA) induced by monosodium urate (MSU). We measured swelling in hind paws vivo using Vernier calipers and ultrasound. liver, kidney, were stained hematoxylin-eosin, M1 type macrophages detected anti-F4/80 anti-iNOS antibodies. mRNA expression inflammatory factors bone marrow-derived (BMDMs) was via quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Nucleotide-binding oligomerization...

In the musculoskeletal system, lymphatic vessels (LVs), which are interdigitated with blood vessels, travel and form an extensive transport network. Blood in bone regulate osteogenesis hematopoiesis, however, whether LVs affect fracture healing is unclear. Here, we investigate draining function at tibial sites using near-infrared indocyanine green imaging (NIR-ICG) discover that drainage insufficiency (LDI) starts on day one persists for up to two weeks following male mice. Sufficient...

Vascular calcification (VC) is a critical pathological condition associated with significant morbidity and mortality. This study employs hybrid approach of network pharmacology molecular biology to delineate the therapeutic mechanisms salidroside (SAL), an active compound from Rhodiola crenulata, against VC. Through database mining analysis, 388 SAL targets intersecting 2871 VC-associated were identified, resulting in 208 common targets. A protein-protein interaction (PPI) constructed via...

The mechanisms underlying Rheumatoid Arthritis (RA) remain unclear. Despite having relatively well-defined treatment strategies, current therapeutic approaches only achieve a remission rate of 70 %-80 %, with poor prognosis and no clear diagnostic criteria for early RA. Therefore, there is need new targets or biomarkers to improve the Firstly, we identified expression characteristics lysozyme (LYZ) in RA patients through plasma proteomics synovial fluid single-cell sequencing analysis....

ABSTRACT Rheumatoid arthritis (RA) is a systemic autoimmune disease. Circular RNA_0001715 (circ_0001715) has been demonstrated to be involved in the progression of cancers. This study aimed discuss function circ_0001715 on development RA. Tumor necrosis factor‐α (TNF‐α) was used active human RA fibroblast‐like synoviocytes (FLS) (HFLS‐RA) cells. The role determined by cell counting kit‐8, flow cytometry, enzyme‐linked immunosorbent assay. target predicted using circinteractome database and...