A5101888669- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Cancer, Hypoxia, and Metabolism
- Neuroscience and Neuropharmacology Research
- Anesthesia and Neurotoxicity Research
- Bone Tissue Engineering Materials
- Pesticide and Herbicide Environmental Studies
- Wnt/β-catenin signaling in development and cancer
- Pluripotent Stem Cells Research
- Cancer-related gene regulation
- Traditional Chinese Medicine Analysis
- Weed Control and Herbicide Applications
- Metabolism, Diabetes, and Cancer
- Heme Oxygenase-1 and Carbon Monoxide
- Fibroblast Growth Factor Research
- Cancer, Stress, Anesthesia, and Immune Response
- RNA modifications and cancer
- Birth, Development, and Health
- Seaweed-derived Bioactive Compounds
- Extracellular vesicles in disease
- Growth Hormone and Insulin-like Growth Factors
- Plant Stress Responses and Tolerance
- Blood groups and transfusion
- RNA Research and Splicing
- Echinoderm biology and ecology
Soochow University
2025
First Affiliated Hospital of Soochow University
2025
Nantong University
2017-2025
Taizhou People's Hospital
2022-2025
Nanjing Medical University
2025
Nanjing Tech University
2022-2024
Kunming University of Science and Technology
2017-2024
Fudan University Shanghai Cancer Center
2022
Children's Hospital of Chongqing Medical University
2021
Chongqing Medical University
2021
MicroRNAs (miRNAs) are initially expressed as long transcripts that processed in the nucleus to yield approximately 65-nucleotide (nt) RNA hairpin intermediates, termed pre-miRNAs, exported cytoplasm for additional processing mature, 22-nt miRNAs. Here, we demonstrate human pre-miRNA nuclear export, and miRNA function, dependent on Exportin-5. Exportin-5 can bind pre-miRNAs specifically vitro, but only presence of Ran-GTP cofactor. Short RNAs, artificial analogs used express small...
Plasmids or viral vectors that express short hairpin RNAs (shRNAs) have emerged as important tools for the stable inhibition of specific genes by RNA interference. shRNAs are structural and functional homologs pre-microRNAs, intermediates in production endogenously encoded microRNAs (miRNAs). Therefore, overexpressed could inhibit miRNA function competing a limiting level one more factors involved biogenesis function. Here, we demonstrate can saturate activity endogenous Exportin 5, factor...
Scribble (Scrib) is a conserved polarity protein required in Drosophila melanogaster for synaptic function, neuroblast differentiation, and epithelial polarization. It also tumor suppressor. In rodents, Scrib has been implicated receptor recycling planar but not apical/basal polarity. We now show that knockdown of disrupts adhesion between Madin-Darby canine kidney cells. As consequence, the cells acquire mesenchymal appearance, migrate more rapidly, lose directionality. Although tight...
RNA activation (RNAa) is a newly discovered mechanism of gene triggered by small double-stranded RNAs termed 'small activating RNAs' (saRNAs). Thus far, RNAa has only been demonstrated in human cells and unclear whether it conserved other mammals. In the present study, we evaluated derived from four mammalian species including nonhuman primates (African green monkey chimpanzee), mouse, rat. Previously, identified saRNAs leading to E-cadherin, p21, VEGF cells. As targeted sequences are highly...
Abstract Purpose: Aberrant activation of β-catenin contributes to the malignant phenotype in hepatocellular carcinoma (HCC). Hypoxia is also known promote HCC invasion and metastasis. However, association between proinvasive role hypoxia remains unclear. We investigated consequences HCC. Experimental Design: established vitro vivo hypoxic models investigate expression cells its hypoxia-induced aggressiveness. The clinical significance and/or factor-1α (HIF-1α) was evaluated using tissue...
The Cdc42 guanosine triphosphatase is essential for cell polarization in several organisms and vitro the organization of polarized epithelial cysts. A long-standing question concerns identity guanine nucleotide exchange factor (GEF) that controls this process. Using Madin–Darby canine kidney cells grown Matrigel, we screened 70 GEFs by RNA interference. Of these, six positives were identified caused a multilumen phenotype, including Tuba, Cdc42-specific GEF localized below apical cortex....
We have previously shown that mechanical strain-induced fetal rat lung cell proliferation is transduced via the phospholipase C-γ-protein kinase C pathway. In present study, we found protein-tyrosine activity of cells increased after a short period strain, which was accompanied by tyrosine phosphorylation proteins ~110-130 kDa. Several components this complex were identified as pp60srcsubstrates. Strain pp60src in cytoskeletal fraction, coincided with shift subcellular distribution from...
Cerebral endothelial cells (CECs) and axons of neurons interact to maintain vascular neuronal homeostasis axonal remodeling in normal ischemic brain, respectively. However, the role exosomes interaction CECs brain under conditions after stroke is unknown.
Abstract Perfluorooctane sulfonate (PFOS) is a fluorinated organic pollutant with substantial accumulation in mammalian liver tissues. However, the impact of chronic PFOS exposure on disease progression and underlying molecular mechanisms remain elusive. Herein, we for first time revealed that micromolar range initiates activation NLR pyrin domain containing 3 (NLRP3) inflammasome to drive hepatocyte pyroptosis. We showed 5 mg/kg/day may exacerbated inflammation steatosis high‐fat diet...
Abstract There are no effective treatments for chemotherapy induced peripheral neuropathy (CIPN). Small extracellular vesicles (sEVs) facilitate intercellular communication and mediate nerve function tumour progression. We found that the treatment of mice bearing ovarian with sEVs derived from cerebral endothelial cells (CEC‐sEVs) in combination a chemo‐drug, oxaliplatin, robustly reduced oxaliplatin‐induced CIPN by decreasing oxaliplatin‐damaged myelination fibres sciatic significantly...
A major pathway by which growth factors, such as platelet-derived factor (PDGF), regulate cell proliferation is via the receptor tyrosine kinase/Ras/mitogen-activated protein kinase (MAPK) signaling cascade. The output of this subjected to tight regulation both positive and negative regulators. One regulator p62(dok), prototype a newly identified family adaptor proteins. We recently provided evidence, through use p62(dok)-deficient cells, that p62(dok) acts factor-induced Ras/MAPK pathway....
Hepatic artery ligation (HAL), transarterial embolization (TAE), and chemoembolization (TACE) have been treatment choices for unresectable hepatocellular carcinoma (HCC). Obstruction of tumor blood supply is one the most important mechanisms these therapeutics measures. Here we introduced HAL into a metastatic human HCC orthotopic nude mouse model (using MHCC97L HepG2 cell lines) to examine effects hepatic flow obstruction on potential cells, investigate underlying effects. Our results...
Increasing evidence suggests that microRNAs, which control gene expression at the post-transcriptional level, are aberrantly expressed in cancers and play significant roles carcinogenesis cancer progression. In this study, we show differential miR-133b down-expression human esophageal squamous cell carcinoma (ESCC) cells tissues. addition, squalene epoxidase (SQLE), a key enzyme of cholesterol synthesis, is identified as direct downstream target by luciferase reporter assay. Furthermore,...
Osteoporotic bone regeneration poses significant challenges due to the complexity of condition. Osteoporosis, a degenerative disorder, results from an imbalance in homeostasis driven by dysregulation osteoblast and osteoclast activity. This complicates treatment osteoporosis its related injuries clinical practice. Despite development various polymer scaffolds for defect repair, achieving effective osteoporotic bones-especially when combined with medications-remains difficult. In this study,...
Mutations in the neurofibromatosis 2 (NF2) gene with resultant loss of expression NF2 tumor suppressor schwannomin are one most common causes benign human brain tumors, including schwannomas and meningiomas. Previously we demonstrated that hepatocyte growth factor-regulated tyrosine kinase substrate (HRS) strongly interacts schwannomin. HRS is a powerful regulator receptor trafficking to degradation pathway also binds STAM. Both these actions for potentially inhibit STAT activation....
There is evidence suggesting that herbal extracts demonstrate greater bioactivities than their isolated constituents at an equivalent dose. This phenomenon could be attributed to the absence of interacting substances present in extracts. By measuring pharmacokinetic parameters paeoniflorin (PF) and albiflorin (AF) after being orally administered rats form, combination with each other within total peony glucosides (TPG), respectively, current study aimed identify positive interactions between...
Rose tea is a kind of popular beverage in many Asian countries. In this study, the contents volatiles, polysaccharides and total polyphenols three species Chinese rose infusions [Kuishui (KSR), Pinying (PYR) Jinbian (JBR)] were determined, respectively. Meantime, volatiles from those analyzed by GC-MS, their antioxidant activities tested 1,1-diphenyl-2-picrylhydrazyl radical scavenging assay. As results, 17, 15, 20 components identified The polyphenols, activity PYR infusion higher than...
Diuron [DU; 3-(3,4-dichlorophenyl)-1,1-dimethylurea], a widely used herbicide for weed control, arouses ecological and health risks due to its environment persistence. Our findings revealed that DU at 0.125-2.0 mg L-1 caused oxidative damage rice. RNA-sequencing profiles disclosed globally genetic expression landscape of rice under treatment. mediated downregulated gene encoding photosynthesis biosynthesis protein, fatty acid, carbohydrate. Conversely, it induced the upregulation numerous...
Nuclear factor erythroid 2-related 2 (Nrf2) protects the lung from sepsis-induced injury through activating Nrf2-regulated multiple phase detoxification genes, including NAD(P)H: quinine oxidoreductase-1 (NQO1) and heme oxygenase-1 (HO1). Based on positive effect of Sirtuin 6 Nrf2, we aim to explore potential role SIRT6 in mechanism acute (ALI).Mouse models sepsis were constructed by instilling intratracheal lipopolysaccharide (LPS; 4 ml/kg). After 48-hour treatment, tissues collected...