A5076677180- Advanced Proteomics Techniques and Applications
- Lysosomal Storage Disorders Research
- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Molecular Biology Techniques and Applications
- Machine Learning in Bioinformatics
- Lipid metabolism and biosynthesis
- Plant and fungal interactions
- Prostate Cancer Treatment and Research
- Parasites and Host Interactions
- Renal cell carcinoma treatment
- Mycotoxins in Agriculture and Food
- Insect and Pesticide Research
- Renal and related cancers
- Redox biology and oxidative stress
- Insect Pest Control Strategies
- Drug Transport and Resistance Mechanisms
- Metabolism and Genetic Disorders
- Genomics and Phylogenetic Studies
- Pancreatic and Hepatic Oncology Research
- 14-3-3 protein interactions
- Bone and Dental Protein Studies
- Enzyme Production and Characterization
- Studies on Chitinases and Chitosanases
- Insect Resistance and Genetics
Victoria University of Wellington
2011-2023
Queen Elizabeth Hospital
2014
University of Otago
2011
Case Western Reserve University
2006
Universitäts-HNO-Klinik Heidelberg
2004
Walter and Eliza Hall Institute of Medical Research
1986
The Royal Melbourne Hospital
1986
Victoria School of Management
1983
Wellington Hospital
1982
Atkins (United States)
1972
This study was undertaken to assess the prognostic effectiveness of Fuhrman nuclear grading and individual components this system, in a series chromophobe renal cell carcinomas. Eighty-seven cases carcinoma were investigated. There 47 males 40 females, 28 78 years age. The carcinomas ranged from 25 180 mm size on TNM staging there 38 stage I, II, 22 III, 2 IV tumors. Whole tumor grade 1, 6 cases; 2, 72 3, 8 4, 1 case, whereas focal (single high power field) case; 62 21 3 cases. On assignment...
Fuhrman grading of renal cell carcinoma focuses on features nuclear size, shape, and nucleolar prominence. Despite the reported widespread usage in clinical studies, there is debate as to prognostic significance reproducibility its criteria. It has been noted that many pathologists rely assessment prominence alone when carcinoma; however, validity this remains unconfirmed. This study was undertaken determine relationship 3 morphologic components system with one another which, if any these,...
This study was undertaken to determine the validity of Fuhrman grading in a series papillary renal cell carcinomas (PRCCs), examine interrelationship and prognostic significance individual components system, further whether any observed predictive value independent other indicators. Ninety cases PRCC were studied. Fifty-nine tumors type 1 31 2. There 33 TNM stage 1, 26 2, 18 3, 12 4 tumors, whereas division according pT category showed 14 pT1a, 20 pT1b, 25 pT2, 15 pT3a, pT3b, 11 pT4 tumors....
Hypochlorous acid (HOCl) is a potent oxidant produced by myeloperoxidase that causes aggregation of many proteins. Treatment apohaemoglobin and apomyoglobin with HOCl regular series oligomer bands when the proteins were separated SDS/PAGE under reducing conditions. Aggregation was detectable at HOCl/protein molar ratio 0.5:1 maximal ratios 10:1–20:1. Dimers formed within 1 min adding HOCl, further occurred over next 30 min. No convincing evidence for covalent cross-linking obtained amino...
Changing antigenic structure such as with capsule polysaccharide is a common strategy for bacterial pathogens to evade host immune system. The recent emergence of an invasive W:2a:P1.7-2,4 sequence type 11 (ST-11) strain Neisseria meningitidis in New Zealand, uncommon serogroup/serotype Zealand disease cases, was investigated its genetic origins. Molecular typing 107 meningococcal isolates similar serotyping characteristics undertaken determine relationships. Results indicated that the had...
Biomarkers are urgently required to support current histological staging provide additional accuracy in stratifying colorectal cancer (CRC) patients according risk of spread properly assign adjuvant chemotherapy after surgery. Chemotherapy is given with stage III reduce the recurrence but controversial II patients. Up 25% will relapse within 5 years tumor removal and when this occurs cure seldom possible. The aim study was identify protein biomarkers stratify CRC Laser micro-dissection used...
A proteomics approach has been used to study changes in protein abundance during leaf senescence white clover. Changes cell ultrastructure were also examined using transmission electron microscopy. The most obvious ultrastructural occurred chloroplasts, with progressive loss of thylakoid integrity and accumulation osmiophilic globules the stroma. Quantitative analysis 590 spots separated by two-dimensional electrophoresis indicated that approximately 40% showed significant related abundance....
Peritoneal macrophages from mice, isolated rat liver Kupffer cells and testis Leydig ingested large numbers of Percoll particles, a gradient medium widely used for separation subcellular organelles by density-gradient centrifugation. A decrease in the percentage adhering to plastic also occurred after exposure Percoll, even at 4 degrees C, temperature which was not ingested. The effect on macrophage adherence may involve loose association between density cell surface. Other...
Plasmodium knowlesi has long been present in Malaysia, and is now an emerging cause of zoonotic human malaria. Cases have confirmed throughout South-East Asia where the ranges its natural macaque hosts Anopheles leucosphyrus group vectors overlap. The majority cases are from Eastern with increasing total public health notifications despite a concurrent reduction falciparum P. vivax implications concerning given highest risk severe fatal disease all spp Malaysia. Current patterns vary based...
ABSTRACT The arrival of eggs in the liver during Schistosoma mansoni infection initiates a protective granulomatous response; however, as progresses, this response results chronic fibrosis. To better understand impact schistosomiasis on function, we used proteomic approach to identify proteins whose expression was significantly altered schistosome-infected mice 8 weeks postinfection. Identification differentially expressed by mass fingerprinting revealed that schistosome markedly reduced...
2-DE and MALDI mass fingerprinting were used to analyse mammary tissue from lactating Friesian cows. The goal was detection of enzymes in metabolic pathways for synthesis milk molecules including fatty acids lactose. Of 418 protein spots analysed by PMF, 328 matched database sequences, resulting 215 unique proteins. We detected 11 out the 15 direct conversion glucose acids, two pentose phosphate pathway lactose glucose. did not detect that catalyse first three reactions glycolysis. Our...
For coniferous gymnosperms, few data exist as to the contribution of membrane-associated proteome cell wall and wood formation. In this study, we begin address knowledge deficiency by examining proteomic profile Golgi-enriched membrane preparations derived from developing Pinus radiata compression wood. These were generated a combination discontinuous sucrose gradient centrifugation Triton X-114-based phase separation. Fractionation separation removed contaminating proteins associated with...
ABSTRACT Novosphingobium nitrogenifigens Y88 T (Y88) is a free-living, diazotrophic Alphaproteobacterium , capable of producing 80% its biomass as the biopolymer polyhydroxybutyrate (PHB). We explored potential utility this species production strain, correlating effects glucose, nitrogen availability, dissolved oxygen concentration, and extracellular pH with changes in proteomic profile. Using two-dimensional differential in-gel electrophoresis tandem mass spectrometry, we identified 217...
A method has been developed for the isolation of a population cells enriched in epithelial lining from bile ducts normal rats. The procedure utilized digestion by pronase white strands biliary and connective tissue which remained after hepatocytes had mechanically removed collagenase-perfused liver. resulting cell was whose ultrastructure resembled that intrahepatic ducts. Contamination with hepatocytes, hepatocyte nuclei erythrocytes less than 2%. have maintained short-term culture. major...
ABSTRACT Changes in cell morphology and adhesion occurred when cultured cells from the rat liver strain C3 were exposed to fungal toxins, sporidesmin or gliotoxin. Both toxins caused loss of attachment plastic tissue culture plates this effect was preceded by actin cables. Other changes included cytoplasmic vacuolation blocked entry into S-phase cycle. Under these conditions [3H]thymidine incorporation also diminished but not detected amount cellular actin, accessibility surface proteins...
Glutaredoxin (thioltransferase) is a thiol-disulfide oxidoreductase that displays efficient and specific catalysis of protein-SSG deglutathionylation thereby implicated in homeostatic regulation the status cellular proteins. Sporidesmin an epidithiopiperazine-2,5-dione (ETP) fungal toxin disrupts functions likely via oxidative alteration cysteine residues on key In current study sporidesmin inactivated human glutaredoxin time- concentration-dependent manner. Under comparable conditions other...
Despite recent advances in surgical techniques and therapeutic treatments, survival from colorectal cancer (CRC) remains disappointing with some 40–50% of newly diagnosed patients ultimately dying metastatic disease. Current staging by light microscopy alone is not sufficiently predictive prognosis would benefit additional support biomarkers order to stratify appropriately for adjuvant therapy. We have identified that cathepsin D expression was significantly greater cells invasive front (IF)...
Abstract— Multiple forms of calf brain N ‐acetyl‐β‐hexosaminidases (β‐2‐acetamido‐2‐ deoxy‐ d ‐glucoside acetamidodeoxyglucohydrolase EC 3.2.1.30) were separated on starch gel electrophoresis at pH 5.8. The organ specific electrophoretic patterns did not depend the cell fraction studied. Much activity is only with difficulty from particulate matter. Two major and one minor component DEAE‐cellulose chromatography Each had both ‐acetyl‐β‐galactosaminidase ‐acetyl‐β‐glucosaminidase activity....
Although there are now multiple methods for the analysis of membrane proteomes, is relatively little systematic characterization proteomic workflows proteins. The Asia Oceania Human Proteome Organisation (AOHUPO) has therefore embarked on a Membrane Proteomics Initiative (MPI) using large range workflows. Here, we describe MPI mouse liver microsomal Standard SDS-PAGE prior to in-gel tryptic digestion and LC-ESI-MS/MS. Na(2) CO(3) wash followed by LC-MS/MS strategy was effective detection...
Selective breeding has strongly reduced the genetic diversity in livestock species and contemporary practices exclude potentially beneficial rare variation from future gene pool. Here we test whether important traits arising by new mutations can be identified rescued highly selected populations. We screened milks 2.5 million cows to identify an exceptional individual which produced milk with saturated fat content improved unsaturated omega-3 fatty acid concentrations. The were transmitted...
Abstract Multiple sclerosis is a disease characterised by axonal demyelination in the central nervous system (CNS). The atypical antipsychotic drug clozapine attenuates experimental autoimmune encephalomyelitis (EAE), mouse model used to study multiple sclerosis, but precise mechanism unknown and could include both peripheral CNS–mediated effects. To better understand where exerts its protective effects, we investigated tissue distribution localisation of using matrix-assisted laser...