A5019890567- Glioma Diagnosis and Treatment
- MRI in cancer diagnosis
- Advanced MRI Techniques and Applications
- Microbial Inactivation Methods
- Radiomics and Machine Learning in Medical Imaging
- Ultrasound and Hyperthermia Applications
- Microfluidic and Bio-sensing Technologies
- Lanthanide and Transition Metal Complexes
- Toxin Mechanisms and Immunotoxins
- Advanced Neuroimaging Techniques and Applications
- Neuroscience and Neural Engineering
- Signaling Pathways in Disease
- Medical Imaging Techniques and Applications
- Brain Metastases and Treatment
- Electroconvulsive Therapy Studies
- Blood Coagulation and Thrombosis Mechanisms
- Electrospun Nanofibers in Biomedical Applications
- Tissue Engineering and Regenerative Medicine
- Medical Imaging and Analysis
- NMR spectroscopy and applications
- Vascular Malformations Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Nanoparticle-Based Drug Delivery
- Mesenchymal stem cell research
- Cell Adhesion Molecules Research
Sheba Medical Center
2014-2023
Tel Aviv University
2007-2017
Mayo Clinic in Arizona
2017
Bar-Ilan University
2008
Magen David Adom
2006
Background— Cell labeling with superparamagnetic iron oxide (SPIO) nanoparticles enables noninvasive MRI and tracking of transplanted stem cells. We sought to determine whether mesenchymal cell (MSC) outcome is affected by SPIO in a rat model myocardial infarction. Methods Results— Rat MSCs were labeled (ferumoxides; Endorem; Guerbet, Villepinte, France). By trypan-blue exclusion assay, almost 100% the cells remained viable after labeling. Seven days MI, rats randomized injections 2×10 6...
Diffusion-weighted magnetic resonance imaging (DWMRI) is sensitive to tissues' biophysical characteristics, including apparent diffusion coefficients (ADCs) and volume fractions of water in different populations. In this work, we evaluate the clinical efficacy DWMRI high diffusion-weighted (HDWMRI), acquired up b = 4000 sec/mm2 amplify sensitivity properties, pretreatment prediction brain tumors' response radiotherapy. Twelve patients with 20 lesions were studied. Six ring-enhancing excluded...
Background— Activated macrophages have a significant role in wound healing and damaged tissue repair. We sought to explore the ability of ex vivo activated promote repair infarcted myocardium. Methods Results— Human macrophage suspension (AMS) was prepared from whole blood unit obtained young donors closed sterile system by novel method hypo-osmotic shock. The AMS (&4×10 5 cells) included up 43% CD14-positive cells injected into ischemic myocardium rats (n=8) immediately after coronary...
Convection-enhanced drug delivery (CED) is a novel approach to delivering drugs into brain tissue. Drugs are delivered continuously via catheter, enabling large volume distributions of high concentrations with minimum systemic toxicity. Previously we demonstrated that CED formation/extent small molecules may be significantly improved by increasing infusate viscosities. In this study show the same methodology can applied monodispersed maghemite nanoparticles (MNPs). For purpose used normal...
Electroporation, is known to induce cell membrane permeabilization in the reversible (RE) mode and death irreversible (IRE) mode. Using an experimental system designed produce a continuum of IRE followed by RE around single electrode we used MRI study effects electroporation on brain. Fifty-four rats were injected with Gd-DOTA treated G25 implanted 5.5 mm deep into striata. was acquired immediately after treatment, 10 min, 20 30 up three weeks following treatment using: T1W, T2W, Gradient...
Conventional magnetic resonance imaging (MRI) is unable to differentiate tumor/nontumor enhancing tissues. We have applied delayed-contrast MRI for calculating high resolution treatment response assessment maps (TRAMs) clearly differentiating tissues in brain tumor patients. One hundred and fifty patients with primary/metastatic tumors were recruited scanned by perfusion MRI. Of those, 47 underwent resection during their participation the study. Region of interest/threshold analysis was...
Abstract Convection-enhanced drug delivery (CED) is a novel approach to directly deliver drugs into brain tissue and tumors. It based on delivering continuous infusion of via intracranial catheters, enabling convective distribution high concentrations over large volumes the target while avoiding systemic toxicity. Efficient formation convection depends various physical physiologic variables. Previous convection-based clinical trials showed significant diversity in extent among patients...
The blood-brain barrier (BBB) is limiting transcellular and paracellular movement of molecules cells, controls molecular traffic, keeps out toxins. However, this protective function the major hurdle for treating brain diseases such as tumors, Parkinson's disease, Alzheimer's etc. It was previously demonstrated that high pulsed electrical fields (PEFs) can disrupt BBB by inducing electroporation (EP) which increases permeability route. Our goal to study effects low PEFs, well below threshold...
The current standard of care for newly diagnosed glioblastoma multiforme (GBM) is resection followed by radiotherapy with concomitant and adjuvant temozolomide. Recent studies suggest that nearly half the patients early radiological deterioration post treatment do not suffer from tumor recurrence but pseudoprogression. Similarly, a significant number brain metastases radiation necrosis following treatments. Conventional MRI currently unable to differentiate progression treatment-induced...
Abstract Background Electroporation-based therapies such as electrochemotherapy (ECT) and irreversible electroporation (IRE) are emerging promising tools for treatment of tumors. When applied to the brain, can also induce transient blood-brain-barrier (BBB) disruption in volumes extending beyond IRE, thus enabling efficient drug penetration. The main objective this study was develop a statistical model predicting cell death BBB induced by electroporation. This be used individual planning....
The development of imaging methodologies for detecting blood-brain-barrier (BBB) disruption may help predict stroke patient's propensity to develop hemorrhagic complications following reperfusion.We have developed a delayed contrast extravasation MRI-based methodology enabling real-time depiction subtle BBB abnormalities in humans with high sensitivity and spatial resolution.The increased is obtained by acquiring T1-weighted MRI at relatively long delays (~15 minutes) after injection...
Despite aggressive therapy, existing treatments offer poor prognosis for glioblastoma multiforme patients, in part due to penetration of most drugs across the blood–brain barrier (BBB). We propose a minimal-invasive combined treatment approach consisting local BBB disruption tumor parallel systemic drug administration. Local is obtained by convection-enhanced delivery novel agent, enabling efficient/targeted systemically administered tumors own vasculature. Various human serum albumin (HSA)...
<h3>BACKGROUND AND PURPOSE:</h3> The interpretation of the radiologic response bevacizumab-treated patients with recurrent high-grade gliomas represents a unique challenge. Delayed-contrast MR imaging was recently introduced for calculating treatment-response-assessment maps in brain tumors, providing clear separation between active tumor and treatment effects. We studied application standard delayed-contrast assessing predicting to bevacizumab. <h3>MATERIALS METHODS:</h3> Twenty-four were...
The blood–brain barrier (BBB) is a major hurdle for the treatment of central nervous system disorders, limiting passage both small and large therapeutic agents from blood stream into brain. Thus, means inducing BBB disruption (BBBd) are urgently needed. Here, we studied application low pulsed electrical fields (PEFs) BBBd in mice. Mice were treated by PEFs using electrodes pressed against sides skull (100–400 square 50 µs pulses at 4 Hz with different voltages). as function parameters was...
Abstract Our aim was to assess the ability of convection-enhanced drug delivery (CED), a novel approach direct drugs into brain tissue and tumors, treat tumors using salirasib (farsnesyl thiosalicylic acid). CED achieved by continuous infusion via intracranial catheters, thus enabling convective distribution high concentrations over large volumes while avoiding systemic toxicity. Several phase II/III CED-based trials are currently in progress but have yet overcome two major pitfalls this...
Abstract The prognosis of Glioblastoma Multiforme patients is poor despite aggressive therapy. Reasons include chemotherapy penetration across the blood-brain barrier and tumor infiltration into surrounding tissues. Here we studied effects combined point-source electroporation (EP) systemic in glioma-bearing rats. 128 rats were studied. Treatment groups administered Cisplatin/Methotrexate before EP (either 90 or 180 pulses). Control treated by EP, chemotherapy, no treatment. Tumor volumes...
Pharmacological treatment of CNS diseases is limited due to the presence blood-brain barrier (BBB). Recent years showed significant advancement in field drug delivery enablers, with technologies such as MR-guided focused ultrasound reaching clinical trials. This have inspired researchers invent novel brain barriers opening (BBo) that are required be simple, fast, safe and efficient. One technology, recently developed by us, BDF (Barrier Disrupting Fields), based on low pulsed electric fields...
Convection-enhanced drug delivery (CED) enables achieving a concentration within brain tissue and tumors that is orders of magnitude higher than by systemic administration. Previous phase I/II clinical trials using intratumoral convection interleukin-4 <i>Pseudomonas</i> exotoxin (PRX321) have demonstrated an acceptable safety toxicity profile with promising signs therapeutic activity. The present study was designed to assess the distribution efficiency this PRX321 magnetic resonance imaging...