A5012852176- Cancer-related gene regulation
- Esophageal Cancer Research and Treatment
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Circular RNAs in diseases
- Ferroptosis and cancer prognosis
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Genetic factors in colorectal cancer
- Esophageal and GI Pathology
- Protein Tyrosine Phosphatases
- Genomic variations and chromosomal abnormalities
- Galectins and Cancer Biology
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Ubiquitin and proteasome pathways
- Pancreatic and Hepatic Oncology Research
- RNA Research and Splicing
- Kruppel-like factors research
- Heat shock proteins research
- Cancer, Lipids, and Metabolism
- Colorectal and Anal Carcinomas
- Lung Cancer Treatments and Mutations
- Cancer-related Molecular Pathways
- Peptidase Inhibition and Analysis
Kunming University of Science and Technology
2015-2025
First People's Hospital of Yunnan Province
2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2007-2023
State Key Laboratory of Molecular Oncology
2012-2017
Peking Union Medical College Hospital
2012
Academy of Medical Sciences
2012
Lung cancer is the first leading cause of cancer-related deaths both worldwide and in China threatens human health quality life. New drugs therapeutic methods are urgently needed. Our study evaluated roles dihydroartemisinin (DHA) lung further explored its underlying mechanisms.CCK-8, colony formation trypan blue exclusion assays were used to detect cell viability, ability death. qRT-PCR Western blotting applied analyze expressions key molecules.DHA inhibited proliferation abilities enhanced...
Bone formation and homeostasis are greatly dependent on the osteogenic differentiation of human bone marrow stem cells (BMSCs). Therefore, revealing mechanisms underlying BMSCs will provide new candidate therapeutic targets for osteoporosis.
Abstract PLK1 is essential for the maintenance of genomic stability during mitosis. In our study, we found that overexpression was an independent prognostic factor (RR = 4.253, p 0.020) and significantly correlated with survivin, antiapoptotic protein, in esophageal squamous cell carcinoma (ESCC). Reverse transcription‐polymerase chain reaction fluorescence situ hybridization (FISH) revealed upregulation mRNA amplification gene, respectively. Depletion activated intrinsic apoptotic pathway,...
Our aim was to identify frequent genomic aberrations in both esophageal squamous cell carcinoma (ESCC) and dysplasia discover important copy number-driving genes microRNAs (miRNA) ESCC.We conducted array-based comparative hybridization (array CGH) on 59 ESCC resection samples 16 biopsy samples. Expression of at 11q13.3 analyzed by real-time PCR (RT-PCR) immunohistochemistry (IHC). Integrated analysis or miRNAs with number-expression correlations.Array CGH identified 11 amplifications eight...
MicroRNAs (miRNAs) play important roles in the progression of human cancer. Although previous reports have shown that miR-145-5p is down-regulated esophageal squamous cell carcinoma (ESCC), and mechanisms down-regulation ESCC are still largely unknown. Using microRNA microarray Gene Expression Omnibus (GEO) datasets, we confirmed was tissues. In vitro assays revealed ectopic expression repressed proliferation, migration, invasion epithelial to mesenchymal transition (EMT). also reduced...
Solute carrier family 7 member 11 (SLC7A11), glutathione peroxidase 4 (GPX4), and apoptosis inducing factor mitochondria associated 2 (AIFM2) are the key regulators in ferroptosis. However, expression patterns prognostic roles of these genes pan-cancer still largely unclear. The SLC7A11, GPX4, AIFM2 relationships between levels immune infiltration were analyzed by using TIMER, gene profiling interactive analysis (GEPIA), Oncomine, Kaplan–Meier databases. Our results showed that both SLC7A11...
MicroRNAs (miRNAs) play important roles in the progression of human cancer including esophageal squamous cell carcinoma (ESCC). Although previous reports showed that miR-125b-5p was down-regulated ESCC, and mechanisms loss function ESCC were still unknown. Using microRNA microarray GEO datasets, we found confirmed tissues. In-vitro assays ectopic expression repressed proliferation, migration invasion, induced senescence. We also reduced expressions cycle regulatory genes CCNA2, CCND1 CCNE1,...
Abstract DNA amplification is one of the mechanisms to activate genes that are implicated in neoplastic transformation and gain chromosome band 3q26 a common event squamous cell carcinomas. The aim present work was identify specific target gene from four candidates ( MDS1 , PRKCI ECT2 PIK3CA ) located on esophageal carcinomas (ESCCs). To assess prevalence copy number gains putative genes, fluorescence situ hybridization (FISH) applied 108 ESCCs 9 ESCC lines. Our data showed were more...
To investigate the molecular mechanisms through which polo-like kinase-1 (PLK1) takes part in anoikis resistance of esophageal squamous cell carcinoma (ESCC) cells.The role PLK1 was examined by ectopic gene expression and siRNA-mediated knockdown. Glutathione S-transferase pull-down co-immunoprecipitation assays were utilized to PLK1-interacting proteins. Electrophoretic mobility shift assay, chromatin immunoprecipitation, reporter carried out identify transcription factors responsible for...
Abstract Oesophageal squamous cell carcinoma (ESCC) is a common and aggressive malignancy. Although many molecular alterations have been observed in ESCC, the mechanisms underlying development progression of this disease remain unclear. In present study, miR-1224-5p was identified to be downregulated ESCC tissues compared normal tissues, its low expression correlated with shorter survival time patients. vitro experiments showed that inhibited proliferation, colony formation, migration...
Esophageal squamous cell carcinoma (ESCC) is one of the most frequent malignant tumors, and mechanisms underlying anti-ferroptosis esophageal cancer cells are still largely unclear. This study aims to explore roles amplified protein kinase C iota (PKCiota) in ferroptosis ESCC cells. Cell viability, colony formation, MDA assay, Western blotting, co-IP, PLA, RNA-seq technologies used reveal PKCiota-induced resistance ferroptosis. We showed here that PKCiota was overexpressed decreased during...
Anoctamin 1 (ANO1) has been found to be overexpressed in esophageal squamous cell carcinoma (ESCC) our previous study. Herein we showed the clinical relevance of ANO1 alterations with ESCC and precancerous lesion progression.ANO1 was detected 38.1% (109/286) 25.4% (77/303) tumors two cohorts, but none morphologically normal operative margin tissues. expression significantly associated a shorter overall survival (OS), especially patients moderately differentiated stage IIA tumors. In 499...
The roles and mechanisms of mini-chromosome maintenance complex component 7 (MCM7) amplification overexpression in esophageal carcinogenesis were investigated. By analyzing the TCGA datasets, we found that MCM7 was amplified approximately 12% squamous cell carcinomas (ESCCs), more than 4% head neck stomach carcinomas. Overexpression further verified three independent GEO datasets cancer. Knockdown using two siRNAs significantly inhibited proliferation, colony formation migration KYSE510...
Abstract Risk assessment of esophageal squamous cell carcinoma (ESCC) is currently based on clinicopathological parameters. To identify genomic markers that can predict overall survival in ESCC, we performed array comparative hybridization (array CGH) a screening set 35 tumor samples from ESCC patients. Prognosis association the genes selected basis CGH results was further validated by real‐time PCR two independent sample sets ( n = 151 and 84). Genomic analysis revealed seven high‐level...
Abstract Protein kinase Cι (PKCι) is an atypical PKC isoform and participates in multiple aspects of the transformed phenotype human cancer cells. We previously reported that frequent amplification overexpression PKCι were correlated with lymph node metastasis primary esophageal squamous cell carcinomas (ESCC). In present study, short interfering RNA–mediated silencing revealed this enzyme was required for migration, invasion, resistance to anoikis. vivo experiments showed suppression...
DNAJB6 is a member of the heat shock protein 40 (Hsp40) family. We here investigated clinical correlation and biological role overexpression in colorectal cancer (CRC). The expression was examined 200 cases adenocarcinomas by immunohistochemistry (IHC) technology. Gene transfection RNA interference were performed to determine effect on invasion CRC cells explore underlying molecular mechanisms vitro vivo. Overexpression found 39% (78/200) tissues, especially tumors at pT4 as compared with...
miR-99a is down-regulated in esophageal squamous cell carcinoma (ESCC), however the role and underlying mechanism are still unknown. We aim to explore of down-regulation ESCC. The expression ESCC tissue
At present, many problems remain to be solved in studying the pathogenesis of thyroid cancer. Ferroptosis is a programmed cell death mode discovered recent years, and studies have found that ferroptosis plays significant role prognosis progression The researchers showed ferroptosis-related genes are essential diagnosing Therefore, this paper summarizes some pathological clinical characteristics cancer makes series combs on relationship between basis function cancer, thus providing specific...
Background/Objectives: MIDN (midnolin) is newly discovered method for critically regulating a ubiquitin-independent proteasomal degradation pathway. This study aims to examine the expression, prognostic value, genomic changes, interacting proteins, methylation status, and correlations with tumor immune microenvironment of in various cancers. Methods: The GTEx, Depmap, GEPIA2, Kaplan–Meier Plotter databases are applied evaluate level normal tissues value genetic alterations cancers...
Wavelength tunable vertical cavity surface emitting lasers (VCSELs) are becoming increasingly popular in wavelength-division multiplexing passive optical networks (WDM-PON). However, due to the limitations of laser beam quality, traditional designs find it difficult achieve stable single mode emission across entire wavelength range. Here, we designed and fabricated an electrically pumped 1060 nm heterogeneous integration (HI) super-span resonance filtering micro-electro-mechanical system...