A5103012508- Plant-Microbe Interactions and Immunity
- Medicinal Plants and Neuroprotection
- Genomics, phytochemicals, and oxidative stress
- Analytical Chemistry and Chromatography
- Phytochemicals and Antioxidant Activities
- Cholinesterase and Neurodegenerative Diseases
- Metabolomics and Mass Spectrometry Studies
- Medicinal Plant Extracts Effects
- Plant Stress Responses and Tolerance
- Plant Parasitism and Resistance
- Phytochemistry and Biological Activities
- Protein Interaction Studies and Fluorescence Analysis
- Lipid Membrane Structure and Behavior
- Membrane Separation Technologies
- Plant pathogens and resistance mechanisms
- Wheat and Barley Genetics and Pathology
- Nicotinic Acetylcholine Receptors Study
- Erythrocyte Function and Pathophysiology
- Avian ecology and behavior
- Mass Spectrometry Techniques and Applications
- RNA and protein synthesis mechanisms
- Water Treatment and Disinfection
- Photoreceptor and optogenetics research
- Botanical Research and Chemistry
- Mycotoxins in Agriculture and Food
Institute of Bioorganic Chemistry, Polish Academy of Sciences
2016-2024
Institute of Plant Genetics, Polish Academy of Sciences
2010-2022
Polish Academy of Sciences
2015-2022
Albert Einstein College of Medicine
2017
University of Life Sciences in Poznań
2017
University of Łódź
1997-2015
KU Leuven
2012-2014
Medical University of Lodz
2014
Cardiff University
2011-2012
Universidad de Guadalajara
2010
Determining the role of plant secondary metabolites in stress conditions is problematic due to diversity their structures and complexity interdependence with different biological pathways. Correlation metabolomic data genetic background provides essential information about features metabolites. LC-MS analysis leaf from 100 barley recombinant inbred lines (RILs) revealed that 98 traits among 135 detected phenolic terpenoid compounds significantly changed level as a result drought stress....
Significance Understanding how host–microbe homeostasis is controlled and maintained in plant roots key to enhance productivity. However, the factors that contribute maintenance of this equilibrium between their multikingdom microbial communities remain largely unknown. Here, we observed a link fungal load health, showed modulation abundance tightly by two-layer regulatory circuit involving host innate immune system on one hand bacterial root commensals another hand. Our results shed light...
Passiflora caerulea L., P. alata Curtis and incarnata L. (synonym for edulis Sims), are the most popular representatives of genus in South America. In recent years, a growing attention is paid to biological activity phytochemical profiles crude extracts from various species worldwide. The aim this study was evaluate compare anti-leukemic dry leaves three greenhouse Poland two human acute lymphoblastic leukemia cell lines: CCRF-CEM its multidrug resistant variant. Two systems liquid...
Abstract Background Rye ( Secale cereale L.) is a cereal crop highly tolerant to environmental stresses, including abiotic and biotic stresses (e.g., fungal diseases). Among these diseases, leaf rust (LR) major threat rye production. Despite extensive research, the genetic basis of immune response LR remains unclear. Results An RNA-seq analysis was conducted examine three unrelated inbred lines (D33, D39, L318) infected with compatible incompatible Puccinia recondita f. sp. secalis Prs )...
Structural analysis via HPLC-ESI-MSn, UPLC-HESI-MS/MS and NMR reported 152 phenolic secondary metabolites in spring barley seedlings (Hordeum vulgare L.). Flavonoids with various patterns of glycosylation acylation, as well hydroxycinnamic acid glycosides, esters amides, were identified methanolic extracts from leaves nine varieties originating different regions the world. Hordatines derivatives, flavones acylated directly on aglycone, hydroxyferulic derivatives deserve special attention....
Melissa officinalis (MO, English: lemon balm, Lamiaceae), one of the oldest and still most popular aromatic medicinal plants, is used in phytomedicine for prevention treatment nervous disturbances. The aim our study was to assess effect subchronic (28-fold) administration a 50% ethanol extract MO leaves (200 mg/kg, p.o.) compared with rosmarinic acid (RA, 10 huperzine A (HU, 0.5 on behavioral cognitive responses scopolamine-induced rats. results were linked acetylcholinesterase (AChE),...
Plants recognize surrounding microbes by sensing microbe-associated molecular patterns (MAMPs) to activate pattern-triggered immunity (PTI). Despite their significance for microbial control, the evolution of PTI responses remains largely uncharacterized. Here, employing comparative transcriptomics six Arabidopsis thaliana accessions and three additional Brassicaceae species investigate responses, we identified a set genes that commonly respond MAMP flg22 exhibit species-specific expression...
Migration is an energetically expensive and hazardous stage of the annual cycle non‐resident avian species, requires certain morphological adaptations. Wing shape one traits that expected to be evolutionarily shaped by migration. Aerodynamic theory predicts long‐distance migrants should have more pointed wings with distal primaries relatively longer than proximal primaries, arrangement minimizes induced drag wing inertia, but this prediction has mostly been tested in passerine species. We...
The construction of useful functional biomolecular components not currently part the natural repertoire is central to synthetic biology. A new light-capturing ultra-high-efficiency energy transfer protein scaffold has been constructed by coupling chromophore centers two normally unrelated proteins: autofluorescent enhanced green fluorescent (EGFP) and heme-binding electron cytochrome b(562) (cyt b(562)). Using a combinatorial domain insertion strategy, variant was isolated in which resonance...
Profiles of flavonoid conjugates present in the root and leaf tissues Mexican wild lupine, Lupinus reflexus, were established using two LC-MSMS systems positive negative ion modes. The trap mass spectrometer quadrupole time-of flight instrument provided sequential MSn spectra MSMS with accurate m/z values [M + H]+ − H] ions, respectively. Sixty-two flavone isoflavone glycoconjugates found tentatively identified. Numerous isomeric or isobaric compounds same molecular could be differentiated....
Membrane biofouling was investigated during the early stages of filtration in a laboratory-scale membrane bioreactor operated on molasses wastewater. The bacterial diversity and composition biofilm activated sludge were analyzed using terminal restriction fragment length polymorphism coupled with 16S rRNA clone library construction sequencing. amount extracellular polymeric substances produced by bacteria spectroscopic methods. results reveal that community differs significantly from...
Eryngium planum L. (EP) is as a rare medicinal plant with lot of potentials pharmaceutical crops. The aim our study was to assess the effect subchronic (28-fold) administration 70% ethanol extract EP roots (200 mg/kg, p.o.) on behavioral and cognitive responses in Wistar rats linked acetylcholinesterase (AChE), butyrylcholinesterase (BuChE), beta-secretase (BACE-1) mRNA levels AChE BuChE activities hippocampus frontal cortex. On last day experiment, 30 min after dose or Huperzine A (HU),...
The site-selective encoding of noncanonical amino acids (NAAs) is a powerful technique for the installation novel chemical functional groups in proteins. This often achieved by recoding stop codon and requires two additional components: an evolved aminoacyl tRNA synthetase (AARS) cognate tRNA. Analysis most successful AARSs reveals common characteristics. highest fidelity NAA systems derived from Methanocaldococcus jannaschii tyrosyl AARS feature specific mutations to residues reported...
The information on the phenotypic and ecological factors that influence hemoglobin concentration in free-living birds is scarce. In order to recognize sources of variation levels migratory shorebirds, we measured whole-blood 553 juvenile 166 adult common snipe Gallinago gallinago during autumn migration through central Poland. Among intrinsic determinants snipe, identified traits such as age, wing morphology, developmental stability, nutritional condition, molt. We found had higher...
Endoplasmic reticulum (ER) bodies are ER-derived structures that contain a large amount of PYK10 myrosinase, which hydrolyzes tryptophan (Trp)-derived indole glucosinolates (IGs). Given the well-described role IGs in root-microbe interactions, we hypothesized ER roots important for interaction with soil-borne microbes at root-soil interface. We used mutants impaired (nai1), body-resident myrosinases (pyk10bglu21), IG biosynthesis (myb34/51/122), and Trp specialized metabolism (cyp79b2b3) to...