Amjad Abu Hasna

ORCID: 0000-0002-1112-985X
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About
Contact & Profiles
Research Areas
  • Endodontics and Root Canal Treatments
  • Dental Radiography and Imaging
  • Dental materials and restorations
  • Essential Oils and Antimicrobial Activity
  • Oral microbiology and periodontitis research
  • Dental Research and COVID-19
  • Dental Trauma and Treatments
  • Oral and Maxillofacial Pathology
  • Dental Implant Techniques and Outcomes
  • Dental Erosion and Treatment
  • Dental Anxiety and Anesthesia Techniques
  • Streptococcal Infections and Treatments
  • Photodynamic Therapy Research Studies
  • Psidium guajava Extracts and Applications
  • Engineering and Material Science Research
  • Bee Products Chemical Analysis
  • Antifungal resistance and susceptibility
  • Sinusitis and nasal conditions
  • Antibiotic Use and Resistance
  • Occupational health in dentistry
  • Scientific and Engineering Research Topics
  • dental development and anomalies
  • Antimicrobial Resistance in Staphylococcus
  • Phytochemistry Medicinal Plant Applications
  • Phytochemical compounds biological activities

Universidade Estadual Paulista (Unesp)
2019-2025

Universidad de Especialidades Espíritu Santo
2023-2025

Weatherford College
2023

Terra
2023

Centro Regional de Derechos Humanos y Justicia de Género, Corporación Humanas
2023

American Association of Endodontists
2022

Institute of Science and Technology
2019-2020

Swinburne University of Technology
1999-2000

Candida spp. cause fungal infection that affects patients' oral health. This study aimed to evaluate the isolated and synergistic antifungal effect of Rosa centifolia L., Curcuma longa Rosmarinus officinalis Punica granatum L. glycolic extracts against albicans, dubliniensis, tropicalis, krusei planktonic biofilm forms. The plant were chemically characterized main compounds quantified by high-performance liquid chromatography (HPLC-DAD) analysis. minimum inhibitory fungicidal concentrations...

10.3390/molecules28062857 article EN cc-by Molecules 2023-03-22

<ns4:p><ns4:bold>Background: </ns4:bold>Sodium hypochlorite (NaOCl) is the most commonly used irrigant in endodontics<ns4:bold>. </ns4:bold>The purpose of this study was to evaluate effect NaOCl solution (2.5%) and gel (3%) with/without passive ultrasonic irrigation (PUI) on <ns4:italic>Enterococcus faecalis, Escherichia coli,</ns4:italic> their endotoxins, lipopolysaccharide (LPS) lipoteichoic acid (LTA).</ns4:p><ns4:p> <ns4:bold>Methods: </ns4:bold>40 human lower premolars were...

10.12688/f1000research.24721.1 preprint EN cc-by F1000Research 2020-06-24

Green propolis may represent a promising therapeutic alternative against dental anaerobic pathogens because of its antimicrobial action. This study aimed to evaluate the and antibiofilm actions Brazilian green aqueous extract (BGP-AqExt) bacteria. The minimum inhibitory concentration (MIC) microbicide (MMC) were determined standard strains (ATCC)

10.3390/molecules27238128 article EN cc-by Molecules 2022-11-22

(1) Background: In hospitals, medical and dental clinics, antiseptics or disinfectants play an essential role in the control of nosocomial infections. This study aimed to evaluate R. officinalis P. paniculata glycolic extracts regarding: (I) their antimicrobial action on planktonic biofilm (monotypic cutaneous model—S. aureus, S. epidermidis C. acnes); (II) cytotoxicity human keratinocytes (HaCaT). (2) Methods: Minimum inhibitory concentration (MIC) minimum bactericidal (MBC) were performed...

10.3390/antibiotics13030226 article EN cc-by Antibiotics 2024-02-28

Background The management of endodontic infections is a complex challenge, mainly due to the involvement diverse microorganisms and their by-products. This study aimed evaluate efficacy N-acetylcysteine (NAC), calcium hydroxide (Ca(OH) 2 ), combined application as intracanal medications in combating Enterococcus faecalis , Escherichia coli lipopolysaccharides (LPS) from E. . Methods A total 60 single-rooted human teeth were carefully selected divided into six groups. These tooth canals...

10.7717/peerj.18331 article EN cc-by PeerJ 2024-10-25

Enterococcus faecalis is strongly associated with secondary/persistent root canal infections, being the most prevalent bacterium in cases of apical periodontitis previously treated teeth. This study was elaborated to evaluate anti-planktonic, antibiofilm, and synergistic effects Nasturtium officinale Psidium guajava hydroethanolic extracts against standard clinical strains E. faecalis. Firstly, N. extract prepared from watercress leaves, P. guava tree leaf shoots. Then, content soluble...

10.3390/app15063178 article EN cc-by Applied Sciences 2025-03-14

Enterococcus faecalis and faecium are frequently associated with persistent endodontic infections due to their ability form biofilms resist conventional treatments. The aim of this study was evaluate the antibacterial antibiofilm effects Lavandula dentata L. essential oil at 100% against standard clinical strains spp. minimum bactericidal concentration (MBC) for each bacterial strain determined. effect evaluated by an MTT assay. Data were statistically analyzed ANOVA Tukey test, a...

10.3390/app15105534 article EN cc-by Applied Sciences 2025-05-15

This study was performed to evaluate the biocompatibility and antifungal effect of Rosmarinus officinalis against Candida albicans in Galleria mellonella model. Five different concentrations R. glycolic extract (50; 25; 12.5 e 6.25 mg/mL) were used its G. model, which nystatin suspension (100; 50; 6.25%) as a control group. The action evaluated on C. for 72, 48 12 h at two phases: (1) using therapeutic agent; (2) prophylactic agent. PBS negative survival curves plotted Kaplan-Meier method...

10.1038/s41598-022-19425-9 article EN cc-by Scientific Reports 2022-09-16

Background The search for novel antimicrobial agents in Endodontics is constant to overcoming persistent infections. Psidium guajava L. a medicinal plant little explored Endodontics. aim of this study was produce hydroethanolic extract P. and evaluate its phytochemical composition, antibiofilm action against standard clinical strains Enterococcus faecalis Candida albicans , cytotoxicity genotoxicity on human keratinocyte cultures (HaCaT cells). findings provide new insights into the...

10.7717/peerj.19301 article EN cc-by PeerJ 2025-04-14

<ns4:p><ns4:bold>Background:</ns4:bold>The purpose of this study was to evaluate the effect different bonding agents on bond-strength demineralized enamel after remineralizing treatments and resin infiltration.</ns4:p><ns4:p><ns4:bold>Methods:</ns4:bold>Buccal 120 bovine incisors polished then were divided into five experimental groups: SE (sound enamel); DE (demineralized AS immersed in artificial saliva for eight weeks); NaF treated with 0.05% sodium fluoride solution (one minute) Ic...

10.12688/f1000research.20523.1 preprint EN cc-by F1000Research 2019-10-11

This study aimed to evaluate sodium hypochlorite (NaOCl), limewater (LW), and Polymyxin B (PMB) as irrigants over MMP-3, MMP-8 MMP-9. Thirty-three patients with apical periodontitis of single-rooted teeth were treated according three-experimental groups (n=11): group-1: 2.5% NaOCl was used irrigant; group-2: for the first two files LW: [0.14% Ca(OH)2] last files; group-3: PMB files. The association Ca(OH)2 CHX an intracanal medication in all groups. Four root canal samplings (S) collected:...

10.1590/0103-6440202003081 article EN cc-by Brazilian Dental Journal 2020-04-01

Root perforation is a common endodontic accident. Its management depends mainly on root canal disinfection and sealing the area by preventing any communication with periodontium to prevent recontamination. A patient was referred treat due previous treatment of tooth #22. The diagnosis symptomatic periapical periodontitis, plan retreat #22 make surgical intervention (apicoectomy) associated antimicrobial photodynamic therapy as complementary technique. Five mineral oxides (5MO) cement used...

10.1155/2020/6677588 article EN cc-by International Journal of Dentistry 2020-12-09

Radiotherapy induces a higher level of Candida spp. colonization, resulting in oral candidiasis. This study aimed to evaluate the phototransformation potential glycolic extract Curcuma longa (C. longa); antifungal activity C. longa, curcumin, and photodynamic therapy (aPDT) with blue light-emitting diodes "LED" on albicans tropicalis vitro; toxicity curcumin Galleria mellonella model. In order confirm light absorption capacity extract, its was evaluated. The effect aPDT evaluated over...

10.1155/2022/5837864 article EN cc-by International Journal of Dentistry 2022-07-01

This study aimed to evaluate the antimicrobial effect of four herbal plants glycolic extracts over mixed-species biofilm composed Candida albicans (C. albicans) and another pathogenic bacterium as alternative therapy be investigated. Four extract Pfaffia paniculata roots; Hamamelis virginiana leaf, Stryphnodendron barbatiman tree bark Gymnema sylvestre stem leaves were tested multi-species C. (ATCC 18804) Streptococcus mutans 35688), Staphylococcus aureus 6538), Enterococcus faecalis 4083)...

10.1111/aej.12681 article EN Australian Endodontic Journal 2022-09-04

The accumulated dental biofilm can be a source of oral bacteria that are aspirated into the lower respiratory tract causing ventilator-associated pneumonia in hospitalized patients. aim this study was to evaluate synergistic antibiofilm action produced and phytochemically characterized extracts Cinnamomum verum Brazilian green propolis (BGP) hydroethanolic against multidrug-resistant clinical strains Acinetobacter baumannii Pseudomonas aeruginosa, addition their biocompatibility on human...

10.3390/molecules28196904 article EN cc-by Molecules 2023-10-01

Teeth with internal root resorption (IRR) have guarded prognosis, considering that IRR defect could influence on the post bond strength. The aim of this study was to evaluate strength and interface between different glass fiber-reinforced posts (FRP) after cementation in teeth simulated (IRR).Forty-five (45) human premolar roots were embedded acrylic resin blocks cross-sectioned into two segments, enabling them be re-approximated by screws. Intracanal medication inserted for 15-days, removed...

10.4317/jced.56568 article EN Journal of Clinical and Experimental Dentistry 2020-01-01

Abstract Calcium silicate-based cements have diverse applications in endodontics. This study aimed to evaluate the antibiofilm action, biocompatibility, morphological structure, chemical composition and radiopacity of Five Mineral Oxides (5MO), Trioxide Aggregate Repair High Plasticity (MTA HP), (MTA) cements. MTT analysis was used test action these against five anaerobic microorganisms, their biocompatibility with mouse macrophage (RAW 264.7) osteoblasts (MG-63) cultures. Their structure...

10.1038/s41598-022-17854-0 article EN cc-by Scientific Reports 2022-08-19

Enterococcus spp. are a common culprit behind the failure of endodontic treatments, primarily due to their notorious resistance antimicrobial agents. Considering this challenge, study was conducted assess efficacy unique blend hydroalcoholic extracts sourced from Salvia officinalis and Glycyrrhiza glabra against biofilms formed by faecalis faecium. The chemical composition these plant rigorously characterized, with primary compound quantification achieved through high-performance liquid...

10.3390/coatings13091579 article EN Coatings 2023-09-10

This study aimed to evaluate the effect of number uses and autoclave sterilization on cyclic fatigue resistance four replica-like instruments RC Blue; Only One File Recip Micro Blue compared original system Reciproc (VDW, Munich, Germany). The were analyzed by scanning electron microscope (SEM) before being used in root canal instrumentation (baseline). Fifty molars instrumented ten (n=10). After an autoclave, SEM. procedure was repeated twice more using different molars, totaling 3 rounds...

10.1038/s41598-023-50096-2 article EN cc-by Scientific Reports 2023-12-27

Abstract Objectives This study investigated the impact of remaining coronal tooth structure on mechanical behavior nonpost (NP) full crowns endodontically treated maxillary central incisors. Materials and Methods Forty bovine incisors with NP 2-mm ferrule were divided into four groups based structure: complete (NP-2), absence mesial distal effect (NP-BL), buccal lingual (NP-MD), no (NP-0). The specimens underwent a stepwise stress fatigue test until fracture occurred, distribution was...

10.1055/s-0043-1776117 article EN cc-by European Journal of Dentistry 2024-03-31
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