A5031841410- Neuroblastoma Research and Treatments
- Cancer, Hypoxia, and Metabolism
- Angiogenesis and VEGF in Cancer
- Chemical Synthesis and Analysis
- Signaling Pathways in Disease
- Renal and related cancers
- Kruppel-like factors research
- Neuropeptides and Animal Physiology
- Receptor Mechanisms and Signaling
- Cancer-related gene regulation
- Photoacoustic and Ultrasonic Imaging
- Developmental Biology and Gene Regulation
- Cancer Genomics and Diagnostics
- Pancreatic and Hepatic Oncology Research
- Cancer Treatment and Pharmacology
- Cellular transport and secretion
- Hepatocellular Carcinoma Treatment and Prognosis
- Cancer Cells and Metastasis
- Hematopoietic Stem Cell Transplantation
- Vascular Tumors and Angiosarcomas
- Acute Lymphoblastic Leukemia research
- Ultrasound and Hyperthermia Applications
- Cell death mechanisms and regulation
- Click Chemistry and Applications
- Chromatin Remodeling and Cancer
Columbia University
2010-2025
Columbia University Irving Medical Center
2016-2025
Columbia College
2002-2022
Royal College of Physicians
2002-2022
NewYork–Presbyterian Hospital
2000-2019
New York Hospital Queens
2016-2019
New York Proton Center
2019
Cancer Research Center
2013-2015
National Center on Addiction and Substance Abuse at Columbia University
2010-2015
Cancer Genetics (United States)
2008-2012
Molecular characterization has the potential to advance management of pediatric cancer and high-risk hematologic disease. The clinical integration genome sequencing into standard practice been limited utility identify clinically impactful information beyond targetable alterations underestimated. Precision in Pediatric Sequencing (PIPseq) Program at Columbia University Medical Center instituted prospective next generation (NGS) for disorders risk treatment failure. We performed whole exome...
Vascular endothelial growth factor (VEGF) plays a key role in human tumor angiogenesis. We compared the effects of inhibitors VEGF with different specificities xenograft model neuroblastoma. Cultured neuroblastoma NGP-GFP cells were implanted intrarenally nude mice. Three anti-VEGF agents tested: an anti-human 165 RNA-based fluoropyrimidine aptamer; monoclonal antibody; and VEGF-Trap, composite decoy receptor based on VEGFR-1 VEGFR-2 fused to Fc segment IgG1. A wide range efficacy was...
Calcium influx is believed to play a critical role in the cascade of biochemical events leading neuronal cell death variety pathological settings, including cerebral ischemia. The synthetic omega-conotoxin peptide SNX-111, which selectively blocks depolarization-induced calcium fluxes through N-type voltage-sensitive channels, protected pyramidal neurons CA1 subfield hippocampus from damage caused by transient forebrain ischemia rat model four-vessel occlusion. SNX-111 provided...
Purpose We conducted a pediatric phase I trial of the vascular endothelial growth factor (VEGF)–neutralizing antibody bevacizumab (BV). Primary aims included estimating maximum-tolerated dose (MTD) and determining dose-limiting toxicities (DLTs), pharmacokinetics, biologic effects BV in children with cancer. Patients Methods (5, 10, 15 mg/kg) was administered intravenously every 2 weeks 28-day courses to refractory solid tumors. Results Twenty-one patients enrolled, 20 (median age, 13 years)...
Vascular endothelial growth factor (VEGF) is a critical promoter of blood vessel during embryonic development and tumorigenesis. To date, studies VEGF antagonists have primarily focused on halting progression in models minimal residual cancer. Consistent with this focus, recent clinical trials suggest that blockade may impede cancer progression, presumably by preventing neoangiogenesis. However, also key mediator endothelial–vascular mural cell interactions, role contribute to the integrity...
One of the early events in pathway receptor-mediated endocytosis is acidification newly formed endocytic vesicle. To examine mechanism acidification, we used fluorescein-labeled alpha 2-macroglobulin (F-alpha 2M) as a probe for vesicle pH. Changes pH were determined from change fluorescein fluorescence at 490-nm excitation measured with microscope spectrofluorometer. After F-alpha 2M, mouse fibroblast cells permeabilized by brief exposure to detergent digitonin. Treatment ionophore monensin...
Abstract Notch signaling is required for vascular development and tumor angiogenesis. Although inhibition of the ligand Delta-like 4 can restrict growth disrupt neovasculature, effect inhibiting receptor function on angiogenesis has yet to be defined. In this study, we generated a soluble form Notch1 (Notch1 decoy) assessed its in vitro vivo. decoy expression reduced stimulated by binding three distinct ligands inhibited morphogenesis endothelial cells overexpressing Notch4. Thus, functioned...
A stage-associated gene expression signature of coordinately expressed genes, including the transcription factor Slug (SNAI2) and other epithelial-mesenchymal transition (EMT) markers has been found present in samples from publicly available datasets multiple cancer types, nonepithelial cancers. The levels co-expressed genes vary a continuous coordinate manner across samples, ranging absence to strong co-expression all genes. These data suggest that tumor cells may pass through an EMT-like...
The biological mechanisms underlying cancer cell motility and invasiveness remain unclear, although it has been hypothesized that they involve some type of epithelial-mesenchymal transition (EMT).We used xenograft models human cells in immunocompromised mice, profiling the harvested tumors separately with species-specific probes computationally analyzing results.Here we show express vivo a precise multi-cancer invasion-associated gene expression signature prominently includes many EMT...
Journal Article Identification of a patient with Bernard-Soulier syndrome and deletion in the DiGeorge/Velo-cardio-facial chromosomal region 22q11.2 Get access Marcia L. Budarf, Budarf * 1Divisions Human Genetics Molecular Biology34th Street Civic Center Boulevard, Philadelphia, PA 19104, USA2Departments PediatricsPhiladelphia, PA, USA *To whom correspondence should be addressed Search for other works by this author on: Oxford Academic PubMed Google Scholar Barbara A. Konkle, Konkle 3The...
Abstract Several hormones, serum proteins, toxins, and viruses are brought into the cell by receptor‐mediated endocytosis. Initially, many of these molecules particles internalized a common endocytic compartment via clathrin‐coated pit pathway. Subsequently, ligands receptors routed to several destinations, including lysosomes, cytosol, or plasma membrane. We have examined mechanism which sorting occurs. A key step in process is rapid acidification vesicles pH 5.0–5.5 This allows...
In the preceding paper (Yamashiro, D. J., and F. R. Maxfield. 1987. J. Cell Biol. 105:2713-2721), we have shown that there is rapid acidification of endosomal compartments to pH 6.3 by 3 min in wild-type Chinese hamster ovary (CHO) cells. contrast, early endosomes markedly reduced CHO mutants, DTF 1-5-4 1-5-1. Since these mutants are pleiotropically defective endocytosis (Robbins, A. R., S. Peng, L. Marshall. 1983. 96:1064-1071; Robbins, C. Oliver, Bateman, Krag, Galloway, I. Mellman. 1984....
Tumor necrosis factor (TNF) was shown previously to stimulate the growth of human FS-4 fibroblasts.Here we show that recombinant TNF can increase binding epidermal (EGF) these cells.Incubation with resulted in a 40-80% number EGF-binding sites no apparent change receptor affinity.The EGF 8-12 h after addition TNF.TNF also increased amount protein immunoprecipitated from cells labeled [35S]methionine.Stimulation synthesis demonstrable 2-4 following treatment.TNF dose-response relationship...
Acidification of endocytic compartments is necessary for the proper sorting and processing many ligands their receptors. Robbins co-workers have obtained Chinese hamster ovary (CHO) cell mutants that are pleiotropically defective in endocytosis deficient ATP-dependent acidification endosomes isolated by density centrifugation (Robbins, A. R., S. Peng, J. L. Marshall. 1983. Cell Biol. 96:1064-1071; Robbins, C. Oliver, Bateman, Krag, Galloway, I. Mellman. 1984. 99:1296-1308). In this following...
Background/Purpose: Vascular endothelial growth factor (VEGF) has been shown previously to correlate with tumor and metastasis in an experimental model of anaplastic Wilms' tumor. The authors hypothesized that treatment anti-VEGF antibodies would suppress both primary this model. Methods: Tumors were induced the right kidneys nude mice by injection cultured cells. After 1 week, was begun either vehicle or antibody intraperitoneally. Mice killed after 4.5 weeks weights incidence metastases...
The potential for avoiding acquired resistance to therapy has been proposed as one compelling theoretical advantage of antiangiogenic based on the normal genetic status target vasculature. However, previous work demonstrated that tumors may resume growth after initial inhibition if blockade is continued an extended period. mechanisms this recurrent are unclear. In these studies, we characterized molecular changes in vasculature during apparent resumption xenograft by vascular endothelial...
We introduce a transport-theory-based PDE-constrained multispectral model for direct imaging of the spatial distributions chromophores concentrations in biological tissue. The method solves forward problem (boundary radiance at each wavelength) and inverse (spatial distribution concentrations), an all-at-once manner framework reduced Hessian sequential quadratic programming method. To illustrate code's performance, we present numerical experimental studies involving tumor bearing mice. It is...