A5101995473- Immunotherapy and Immune Responses
- Birth, Development, and Health
- NF-κB Signaling Pathways
- Immune Cell Function and Interaction
- Electrolyte and hormonal disorders
- Renin-Angiotensin System Studies
- Monoclonal and Polyclonal Antibodies Research
- interferon and immune responses
- Ubiquitin and proteasome pathways
- Neuroendocrine regulation and behavior
- Adenosine and Purinergic Signaling
- Neuroscience of respiration and sleep
- Ocular Oncology and Treatments
- Pregnancy and preeclampsia studies
- Cancer Immunotherapy and Biomarkers
- Metabolomics and Mass Spectrometry Studies
- Gastrointestinal motility and disorders
- Smoking Behavior and Cessation
- Immune cells in cancer
- Plant biochemistry and biosynthesis
- Occupational and environmental lung diseases
- Extracellular vesicles in disease
- Ion Channels and Receptors
- Liver Disease Diagnosis and Treatment
- Nicotinic Acetylcholine Receptors Study
Zhejiang University of Technology
2024
Guangzhou Medical University
2024
Shanghai Cell Therapy Research Institute
2024
Hangzhou Red Cross Hospital
2018-2023
Soochow University
2008-2022
Huazhong University of Science and Technology
2018-2022
Zhejiang University
2022
Jiangsu University
2021
First People's Hospital of Kunshan
2021
Fuzhou University
2015-2018
Abstract Our current understanding of how sugar metabolism affects inflammatory pathways in macrophages is incomplete. Here, we show that glycogen an important event controls macrophage-mediated responses. IFN-γ/LPS treatment stimulates to synthesize glycogen, which then channeled through glycogenolysis generate G6P and further the pentose phosphate pathway yield abundant NADPH, ensuring high levels reduced glutathione for macrophage survival. Meanwhile, also increases UDPG receptor P2Y 14...
Abstract Hypoxia is known to be commonly present in breast tumor microenvironments. Stem-like cells that repopulate tumors, termed tumor-repopulating (TRC), thrive under hypoxic conditions, but the underlying mechanism remains unclear. Here, we show hypoxia promotes growth of TRCs through metabolic reprogramming. mobilized transcription factors HIF1α and FoxO1 induced epigenetic reprogramming upregulate cytosolic phosphoenolpyruvate carboxykinase (PCK1), a key enzyme initiates...
Tumor cell-derived microparticles (T-MP) contain tumor antigen profiles as well innate signals, endowing them with vaccine potential; however, the precise mechanism by which DCs present T-MP antigens to T cells remains unclear. Here, we show that T-MPs activate a lysosomal pathway is required for presenting of T-MPs. endocytose lysosomes, where increase pH from 5.0 peak 8.5 via NOX2-catalyzed reactive oxygen species (ROS) production. This increased pH, coupled T-MP-driven centripetal...
Clinical applications of antiangiogenic agents profoundly affect tumor cell behaviors via the resultant hypoxia. To date, how hypoxia regulates cells remains unclear. Here, we show that promotes growth human breast tumorigenic repopulate tumors [tumor-repopulating (TRCs)] in vitro and vivo. This stimulating effect is ascribed to hypoxia-induced reactive oxygen species (ROS) activates Akt NF-κB, dependent on attenuated tricarboxylic acid (TCA) cycle. We find fumarate accumulated TCA cycle...
Abstract Malignant pleural effusion (MPE) is a frequent complication of various cancers and often leads to poor quality life, prognosis, life expectancy, its management remains palliative. New approaches that can effectively treat MPE are highly desirable. Here, we show methotrexate (MTX)-packaging tumor cell–derived microparticles (MTX-MP) act as an effective immunotherapeutic agent patients with by mobilizing activating neutrophils. We find MTX-MP perfusion via catheter elicits the...
Glycolysis facilitates the rapid recall response of CD8+ memory T (Tm) cells. However, it remains unclear whether Tm cells uptake exogenous glucose or mobilize endogenous sugar to fuel glycolysis. Here, we show that intracellular glycogen rather than extracellular acts as major carbon source for early response. Following antigenic stimulation, exhibit high phosphorylase (brain form, PYGB) activity, leading glycogenolysis and release glucose-6-phosphate (G6P). Elevated G6P mainly flows...
High-salt intake has been demonstrated in link to hypertension, and cardiovascular diseases could be programmed fetal origins. We determined the influence of high-salt diet during pregnancy on development heart.Fetal cardiac structures, cell cycle, renin-angiotensin system (RAS), epigenetic alternations heart following maternal high salt were examined.Following exposure salt, disorganized myofibrillae mitochondria cristae loss found fetus, S-phase for cells was enhanced, plasma angiotensin...
Abstract The pathogenesis of pain in irritable bowel syndrome (IBS) is poorly understood and treatment remains difficult. present study was designed to investigate roles adrenergic signaling the endogenous hydrogen sulfide producing enzyme cystathionine β-synthetase (CBS) a previously validated rat model IBS induced by neonatal colonic inflammation (NCI). Here we showed that NCI-induced visceral hypersensitivity (VH) significantly attenuated β 2 subunit inhibitor but not 1 or 3 α inhibitor....
Mevalonate is an important platform compound for the biosynthesis of isoprenoids. It can be synthesized from acetyl-CoA in presence nicotinamide adenine dinucleotide phosphate (NADPH) by introduced mvaES operon Escherichia coli. The influences E. coli hosts, supply, and NADPH availability were assessed engineered to improve production titer yield mevalonate glycerol. As a result, DH5α was found best host with high specific capability Through engineering phosphoketolase-phosphotransacetylase...
Platycodin D (PD) is a potent adjuvant with dual Th1 and Th2 potentiating activity, but its mechanisms of action remain unclear. Here, the C2C12 myoblast cell line mice were used as in vitro vivo models to identify potential signaling pathways involved activity PD. PD induced transient cytotoxicity inflammatory response cells mouse quadricep muscles. A comparative analysis microarray data revealed that similar gene expression profiles muscles, triggered rapid regulation death, immune,...
Rheumatoid arthritis (RA) is a chronic autoimmune disease affecting nearly 1% of adults worldwide. This study aimed to investigate whether sophocarpine potential drug for treating RA. The cytotoxicity RA-fibroblast-like synoviocytes (FLSs) was evaluated using 3-[4,-dimethylthiazol-2-y]-2,5-diphenyl-tetrazolium bromide (MTT) assays kit and released lactate dehydrogenase (LDH) assays. transcription proinflammatory cytokines in RA-FLSs analyzed by reverse real-time polymerase chain reaction...