Magnesium oxide nanostructured particles (NP) were prepared using a simple solution combustion technique different leaf extracts such as Mangifera indica (Mango - Ma), Azadirachta (Neem-Ne), and Carica papaya (Papaya-Pa) surfactants. The highly crystalline phase of MgO nanostructures was confirmed by PXRD FTIR studies for 2 h 500°C calcined samples. To analyze the characteristics obtained material-MaNP, NeNP, PaNP dosimetry applications, thermoluminescence (TL) carried out Co-60 gamma rays...

Infectious disease is one of the greatest causes morbidity and mortality worldwide, with emergence antimicrobial resistance, situation worsening. In order to prevent this crisis, resistance needs be monitored carefully control spread multidrug-resistant bacteria. Therefore, in study, we aimed determine prevalence infection caused by Klebsiella pneumoniae investigate profile pattern K. last eleven years. This retrospective study was conducted a tertiary hospital Makkah, Saudi Arabia. Data...

Acinetobacter baumannii is pathogen of global concern. It causes infection, especially among immunocompromised individuals in intensive care units, due to its ability survive for long periods on hard surfaces and under a wide range environmental conditions become resistant almost all the available antibiotics used clinical practice. Objectives: This study aims address gap A. surveillance Saudi Arabia by tracking prevalence, patterns, trends acquired resistance at healthcare facility western...

Two putative autotransporter proteins, CapA and CapB, were identified in silico from the genome sequence of Campylobacter jejuni NCTC11168. The genes encoding each protein contain homopolymeric tracts, suggestive phase variation mediated by a slipped-strand mispairing mechanism; case gene contained frameshifts at these positions. C-terminal two-thirds two genes, as well portion predicted signal peptides, identical; remaining N-terminal portions specific. Both cloned expressed; recombinant...

Arabian flora is a rich source of bioactive compounds. In this study, we investigated three aromatic plant species with the aim finding valuable sources antimicrobial agents against common pathogenic microorganisms. We focused especially on microorganisms, which cause outbreaks infectious disease during mass gatherings and pilgrimages season in Saudi Arabia. The essential oils were hydrodistilled from flowering aerial parts Lavandula pubescens Decne. Pulicaria incisa subsp. candolleana...

Pseudomonas aeruginosa (P. aeruginosa) is a common causative pathogen in healthcare settings and displays increasing levels of resistance to antimicrobial drugs. Its capacity resist has been reported multiple locations across the world. This study evaluates current antibiotic seeks understand patterns context clinical isolates P. aeruginosa.All were cultured at 37 °C for 24 h different media: blood sheep agar, McConkey cystine-lactose-electrolyte-deficient agar (CLED), bacterial...

Helicobacter pylori (H. pylori) infection is reported to be associated with various extragastrointestinal conditions such as insulin resistance, diabetes mellitus and metabolic syndrome. H. type 2 (T2DM) are oxidative stress, this cross-relation between induced in T2DM damage still debated. Thus, the question arises whether an increase serum level of 8-OHdG Ox-LDL will occurs patients infected pylori; through determination compare frequency non-diabetic patients. 100 presented history...

Bixa orellana (B. Orellana) is a frequently utilized plant that has grown in significance pharmaceutical applications. The leaves extract of B. was used the current study for preliminary phytochemical analysis terms both quantitative and qualitative, which indicates presence phenols, alkaloids, flavonoids. Furthermore, demonstrated antifungal activity at 30 mm zone clearance against Candida albicans antibacterial 16 Bacillus nakamuria by well diffusion method different strains. showed MIC 20...

This study encapsulates an in-depth correlation analysis for the biological activities (cytotoxicity, antimicrobial, and α-amylase inhibition) vs. phytochemical classes (flavonoids "FV" alkaloid "AL") present in fenugreek seed extract. Cell cultures different cell lines were used to assess cytotoxicity selectivity (IC50 value), agar diffusion assay was determine MIC MBC bacteria fungi, whereas inhibition studied evaluate antidiabetic potential forty-five origins of extracts. An in-house...

Objective To determine risk factors for multi-drug-resistant Acinetobacter baumannii (MDR-AB) nosocomial infections in intensive care units a tertiary hospital, Makkah, Saudi Arabia. Methods We performed hospital-based, matched case–control study patients who were admitted to Al Noor Specialist Hospital between 1 January 2012 and 31 August 2012. The included cases of A. infection controls without infection. Controls by age ward admission. Results most frequent site was the respiratory tract...

Antimicrobial stewardship programs (ASPs) are collaborative efforts to optimize antimicrobial use in healthcare institutions through evidence-based quality improvement strategies. The general administration of pharmaceutical care the Saudi ministry health (MOH) is putting outstanding implementing settings. Several surveys have been conducted globally and reported many types strategies their effectiveness. This study aims identify ASPs Makkah region hospitals perceived level success. We...

In this study, a new series of 4-(2,5-dimethyl-1H-pyrrol-1-yl)-N’-(2-(substituted)acetyl) benzohydrazides (5a–n) were prepared and heterocycles underwent thorough characterization evaluation for antibacterial activity; some them further testing in vitro inhibition enoyl ACP reductase DHFR enzymes. The majority the synthesized molecules exhibited appreciable action against Some compounds also showed strong antitubercular properties. order to determine potential mode compounds, molecular...

Background: Antimicrobial stewardship programs (ASPs) are collaborative efforts to optimize antimicrobial use in healthcare institutions through evidence-based quality improvement strategies. With regard critically ill patients, appropriate usage is of significance, and any delay therapy increases their risk mortality. Therefore, the implementation structured multidisciplinary ASPs critical care settings utmost importance promote judicious antimicrobials. Methods: This quasi-experimental...

The ability and potency of bacterial species to form biofilms, which show antibiotic resistance thereby avoiding surfaces, is a major cause prolonged infections. Various advanced approaches have been employed prevent or damage formed by variety strains, help the associated infectious disease. In this context, zinc-based nanostructures recognized as potential agent against broad spectrum communities. As result, sustainable green synthesis method was adapted in present study synthesize...

To determine the prevalence of Helicobacter pylori infection in a Saudi Arabian population and its association with body mass index (BMI) serum 8-hydroxy deoxyguanine (8-OHdG) levels as biomarker for oxidative stress.This cross-sectional study enrolled patients that had experienced epigastric discomfort or dyspepsia > 1 month undergone diagnostic upper endoscopy. Patients ≥30 kg/m2 were defined obese. The presence anti-H. antibodies was confirmed using an H. immunoglobulin G (IgG) antibody...

To explore inhibitory effects of genome-specific, chemically synthesized siRNAs (small interference RNA) against NS3 gene hepatitis C virus (HCV) 1a genotype in stable Huh-7 (human hepatoma) cells as well viral replication serum-inoculated cells. Stable persistently expressing were produced under antibiotic gentamycin (G418) selection. The cell clones resistant to 1 000 μg concentration picked clones. expression clone was confirmed by RT-PCR and Western blotting. siRNA cytotoxicity...

Objective: To detect the prevalence of carbapenem resistance among Gram negative bacilli at Al-Noor Specialist Hospital in western part Saudi Arabia. Materials and Methods: This study was conducted Microbiology department, Hospital, Makah city, The clinical samples were collected from admitted patients wards, Intensive Care Units (ICUs), urology unit surgery unit, these included blood, urine, sputum, pus wound. identification antibiotic susceptibility testing all bacterial isolates performed...

Nowadays, the emergence of drug resistance was a major problem due to limited bioavailability and unintended toxicity antibiotics because non-specific targeting leading difficulty eradicate pathogenic infections. Zinc oxide nanoparticles gain much attention as nanocarriers, their non-toxic, eco-friendly, economical cost, treat severe The purpose this research produce analyze zinc (ZnONPs) functions using green synthesis process evaluate its anti-bacterial biofilm inhibitory activities...

Streptococcus pneumoniae is one of the major precarious pathogens accountable for over 1.2 million fatalities annually. The key drivers pneumococcal vaccine development involve high morbidity and mortality in cases, especially very young children elderly. In this study, immunoinformatics was integrated with subtractive proteomics to find antigenic proteins designing a multi-epitope against S. pneumoniae. As prospective targets, developed pipeline identified two proteins, i.e.,...

ABSTRACT Multidrug-resistant (MDR) Gram-negative infections represent a growing problem and serious global threat. Carbapenem-resistant Klebsiella pneumoniae is perhaps cause the most difficult infection to treat associated with increased morbidity mortality. Here, we report draft genome sequence of an MDR K. strain isolated from Makkah, Saudi Arabia.

The aim of this study was to the evaluation frequency and distribution Serratia marcescens in hospital departments determination antimicrobial resistance isolated strains. Methods: included 81 strains from 61 patients hospitalized different wards Al-Noor Specialist Hospital within period 1/11/2012 1/11/2013. were wound swabs, blood cultures, sputum, urine culture, fluid, catheter throat swab, cultures cerebrospinal fluid. Results: isolates identified by conventional method results...