A5022555461- Phenothiazines and Benzothiazines Synthesis and Activities
- Quinazolinone synthesis and applications
- Bacteriophages and microbial interactions
- Tuberculosis Research and Epidemiology
- Research on Leishmaniasis Studies
- Mycobacterium research and diagnosis
- Genomics and Phylogenetic Studies
- Antibiotic Resistance in Bacteria
- Diabetic Foot Ulcer Assessment and Management
- Microbial infections and disease research
- Wound Healing and Treatments
- Trypanosoma species research and implications
- Cancer therapeutics and mechanisms
- Antibiotics Pharmacokinetics and Efficacy
- Rabies epidemiology and control
- Antimicrobial Resistance in Staphylococcus
- Synthesis and Biological Evaluation
- Bacterial Genetics and Biotechnology
- Pressure Ulcer Prevention and Management
- Mesenchymal stem cell research
- Pharmaceutical and Antibiotic Environmental Impacts
- Antimicrobial agents and applications
- Carbohydrate Chemistry and Synthesis
- Clostridium difficile and Clostridium perfringens research
- bioluminescence and chemiluminescence research
TechnoPhage (Portugal)
2012-2024
Hospital de Egas Moniz
2014-2015
University of Lisbon
2004-2007
Universidade Nova de Lisboa
2001-2005
Instituto de Medicina Tropical
2005
ABSTRACT The phenothiazines chlorpromazine (CPZ) and thioridazine (TZ) have equal in vitro activities against antibiotic-sensitive -resistant Mycobacterium tuberculosis. These compounds not been used as anti- M. tuberculosis agents because their take place at concentrations which are beyond those that clinically achievable. In addition, chronic administration of CPZ produces frequent severe side effects. Because has shown to enhance the killing intracellular medium relevant, we investigated...
Abstract Chronic wounds that fail to heal are a common complication of diabetes mellitus and the most precipitating reason for nontraumatic lower limb amputation. Unfortunately, bacterial species cause these infections becoming more resistant antibiotics, making them increasingly difficult treat. We assessed feasibility combating chronic with topically delivered bacteriophage cocktail in two animal models mellitus. Microbiological, planimetric, histological parameters were compared debrided...
Expression of eight transporter genes Escherichia coli K-12 and its DeltaacrAB mutant prior to after induction both strains tetracycline resistance reversal induced were analyzed by quantitative reverse transcriptase PCR. All overexpressed with acrF being 80-fold more expressed in the tetracycline-induced strain.
ABSTRACT An American Type Culture Collection reference strain and eight clinical strains of Mycobacterium tuberculosis , all which were susceptible to isoniazid (INH) (mean MIC, 0.06 mg/liter) negative for the Ser315Thr katG mutation, left in their BACTEC 12B vials (for use with 460-TB method) containing 0.1 mg INH per liter periods up 28 days after completion antibiotic susceptibility test. Each eventually grew levels compatible those INH-resistant strains. Successive passages...
In patients with diabetes mellitus, foot infections pose a significant risk. These are complex commonly caused by Staphylococcus aureus, Pseudomonas aeruginosa and Acinetobacter baumannii, all of which potentially susceptible to bacteriophages. Here, we characterized five bacteriophages that had determined previously have antimicrobial wound-healing potential in chronic S. P. A. baumannii infections. Morphological genetic features indicated the were lytic members family Myoviridae or...
Due to their bacterial lytic action, bacteriophage endolysins have recently gained great attention as a potential alternative antibiotics in the combat of Gram-positive pathogenic bacteria, particularly those displaying multidrug resistance. However, large-scale production and purification is frequently impaired due low solubility. In addition, large number appear exhibit reduced efficacy when compared with action during phage infection. Here, we took advantage high solubility two...
ABSTRACT The INNO-LiPA Rif.TB assay for the identification of Mycobacterium tuberculosis complex strains and detection rifampin (RIF) resistance has been evaluated with 360 smear-positive respiratory specimens from an area high incidence multidrug-resistant (MDR-TB). sensitivity when compared to conventional identification/culture methods was 82.2%, specificity 66.7%; were 100.0% 96.9%, respectively, RIF resistance. This potential provide rapid information that is essential effective...
Increasing antibiotic resistance of bacterial pathogens has drawn the attention to potential use bacteriophage endolysins as alternative antibacterial agents. Here we have identified, characterized, and studied lytic two endolysins, Lys168 Lys170, from phages infecting Enterococcus faecalis. Lys170 belong cysteine, histidine-dependent amidohydrolases/peptidases (CHAP) amidase-2 protein families, respectively. is quite a unique enterococcal phage endolysin. It shares 95% amino acidic identity...
Bacteriophage endolysins are bacterial cell wall degrading enzymes whose potential to fight infections has been intensively studied. Endolysins from Gram-positive systems typically described as monomeric and having a modular structure consisting of one or two N-terminal catalytic domains (CDs) linked C-terminal region responsible for binding (CWB). We show here that expression the endolysin gene lys170 enterococcal phage F170/08 results in products, expected full length (Lys170FL) fragment...
Abstract Although alkaloids from the family Aizoaceae have anticancer activity, species of this received little attention. Because these also exhibit properties normally associated with compounds that activity at level plasma membrane, a methanol extract Carpobrotus edulis, common plant found along Portuguese coast, was studied for membrane active compounds. The results study show is non‐toxic concentrations inhibit verapamil sensitive efflux pump L5178 mouse T cell lymphoma line thereby...
This study evaluated T cell immune responses to purified protein derivative (PPD) and Mycobacterium tuberculosis (Mtb) in health care workers who remained free of active (HCWs w/o TB), went on develop TB w/TB), non-health were (Non-HCWs) patients presenting with minimal (Min TB) or advanced (Adv disease. Peripheral blood mononuclear cells (PBMC) stimulated Mtb PPD the expression activation markers CD25+ HLA-DR+, intracellular IL-4 IFN-γ production cytotoxic evaluated. PBMC from HCWs...
Abstract Background Diabetic Foot Ulcers (DFU) are a major complication in patients with diabetes and affect globally 170 million patients. Approximately 28% of infected DFU require amputation. foot infections (DFI) caused by persistent or multidrug resistant organisms threat to the outcome therapy. Infections mostly polymicrobial, pathogens include Staphylococcus aureus Pseudomonas aeruginosa. A promising new approach using phages may potentially be novel modality increase treatment...