A5003518645- Epigenetics and DNA Methylation
- Cancer Genomics and Diagnostics
- Genetic factors in colorectal cancer
- Cancer-related gene regulation
- RNA modifications and cancer
- Wnt/β-catenin signaling in development and cancer
- Ubiquitin and proteasome pathways
- Colorectal Cancer Screening and Detection
- Ovarian cancer diagnosis and treatment
- Acute Myeloid Leukemia Research
- Pancreatic and Hepatic Oncology Research
- Cancer, Hypoxia, and Metabolism
- Endometrial and Cervical Cancer Treatments
- Hernia repair and management
- Gastrointestinal disorders and treatments
- Colorectal Cancer Treatments and Studies
- Genomics and Chromatin Dynamics
- Immunodeficiency and Autoimmune Disorders
- Intraperitoneal and Appendiceal Malignancies
- Colorectal Cancer Surgical Treatments
- Hormonal Regulation and Hypertension
- Histone Deacetylase Inhibitors Research
- Kruppel-like factors research
- Oral and Maxillofacial Pathology
- Colorectal and Anal Carcinomas
The University of Tokyo
2018-2024
Chiba University
2014-2018
Nihon University
2013-2017
Abstract Aberrant DNA methylation is a common epigenetic alteration involved in colorectal cancer ( CRC ). In our previous study, we performed methylated immunoprecipitation‐on‐chip analysis combined with gene re‐expression by 5‐aza‐2′‐deoxycytidine treatment, to identify genes genome widely. Among these genes, 12 showed aberrant hypermethylation frequently >75% of 149 samples but did not normal samples. this aim find out any be utilized for detection using plasma Primers...
To clarify molecular alterations in serrated pathway of colorectal cancer (CRC), we performed epigenetic and genetic analyses sessile adenoma/polyps (SSA/P), traditional adenomas (TSAs) high-methylation CRC. The methylation levels six Group-1 14 Group-2 markers, established our previous studies, were analyzed quantitatively using pyrosequencing. Subsequently, targeted exon sequencing 126 candidate driver genes examined that are associated with development. SSA/P showed high both frequent...
Abstract Deregulation of the Wnt/β-catenin pathway is associated with development human cancer including colorectal and liver cancer. Although we previously showed that histidine ammonia lyase ( HAL ) was transcriptionally reduced by β-catenin/TCF complex in cells, mechanism(s) its down-regulation remain to be clarified. In this study, search for transcription factor(s) regulating , identify CEBPA FOXA1, two factors whose expression suppressed knockdown β-catenin or TCF7L2. addition, RNA-seq...
Bromodomain-containing protein 8 (BRD8) is a subunit of the NuA4/TIP60-histone acetyltransferase complex. Although BRD8 has been considered to act as co-activator complex, its biological role remains be elucidated. Here, we uncovered that accumulates in colorectal cancer cells through inhibition ubiquitin-dependent degradation by interaction with MRG domain binding protein. Transcriptome analysis coupled genome-wide mapping BRD8-binding sites disclosed transactivates set genes independently...
<b><i>Introduction:</i></b> Pseudomyxoma peritonei (PMP) is a disease characterized by progressive accumulation of intraperitoneal mucinous ascites produced neoplasms in the abdominal cavity. Since prognosis patients with PMP remains unsatisfactory, development effective therapeutic drug(s) matter pressing concern. Genetic analyses have clarified frequent activation <i>GNAS</i> and/or <i>KRAS</i>. However, involvement global epigenetic...
Abstract Background Abnormal activation of Wnt/β‐catenin signaling is associated with various aspects cancer development. This study explored the roles novel target genes pathway in cells. Methods Using haploid chronic myelogenous leukemia cell line HAP1, RNA sequencing (RNA‐seq) was performed to identify whose expression increased by APC disruption and reversed β‐catenin knockdown (KD). The regulatory mechanism function one candidate investigated colorectal (CRC) Results In total, 64...
Abstract Sporadic colorectal cancer ( CRC ) is classified into several molecular subtypes. We previously established two groups of DNA methylation markers through genome‐wide analysis to classify distinct subgroups: high‐, intermediate‐, and low‐methylation epigenotypes HME , IME LME respectively). also called CpG island methylator phenotype CIMP )‐high shows both Group 1 markers) 2 markers, while / ‐low 2, but not no either or markers. While BRAF ‐ KRAS mutation(+) strongly correlated with...
Although most sporadic colorectal cancers ( CRC ) are thought to develop from protruded adenomas through the adenoma–carcinoma sequence, some flat lesions, so‐called laterally spreading tumors LST ). We previously analyzed epigenetic aberrations in and found that clearly classified into two molecular subtypes: intermediate‐methylation with KRAS mutation low‐methylation absence of oncogene mutation. Intermediate‐methylation were mostly granular type ‐G) non‐granular ‐ NG In present study, we...
Although liquid-based cytology (LBC) has increased the sensitivity of cytological diagnosis endometrial cancer (EC) compared with conventional smear cytology, LBC for detection EC is between 70% and 96% remains unsatisfactory. In present study, we efficacy genetic (LBGDx) by amplicon sequencing five genes including PTEN, PIK3CA, CTNNB1, KRAS, TP53 in 48 subjects who underwent screening. Consequently, classified 15 samples as "positive or suspicious malignancy" were later confirmed EC....
Isocitrate dehydrogenase 1 and 2 ( IDH1/2 ) mutations their key effector 2-hydroxyglutarate (2-HG) have been reported to promote oncogenesis in various human cancers. To elucidate molecular mechanism(s) associated with mutations, we established mouse embryonic fibroblasts (MEF) cells colorectal cancer stably expressing cancer-associated IDH1 R132C or IDH2 R172S , analyzed the change metabolic characteristics of these cells. We found that mutants induced intracellular 2-HG accumulation...
Aberrant DNA methylation causes major epigenetic changes and has been implicated in cancer following the inactivation of tumor suppressor genes by hypermethylation promoter CpG islands. Although methylated regions can be randomly demethylated 5-azacytidine 5-aza-2'-deoxycytidine, site-specific inhibition methylation, for example, region a specific gene, yet to technically achieved. Hairpin pyrrole (Py)-imidazole (Im) polyamides are small molecules that designed recognize bind particular...
Aberrant activation of the Wnt/β‑catenin signaling pathway plays a crucial role in development and progression colorectal cancer. Previously, we identified set candidate genes that were regulated by this pathway, present study focused on motile sperm domain containing 1 (<em>MOSPD1</em>). Immunohistochemical staining revealed expression MOSPD1 was elevated tumor cells from cancer tissues compared with non‑tumor cells. Using ChIP‑seq data JASPAR database, regulatory region(s) <em>MOSPD1</em>...
While sporadic colorectal cancer (CRC) is classified into several molecular subtypes, stratification of familial tumors yet to be well investigated. We previously established two groups methylation markers through genome-wide DNA analysis, which CRC and adenoma three distinct subgroups: high-, intermediate-, low-methylation epigenotypes. Here, we investigated adenomatous polyposis (FAP), quantitative analysis 127 samples (16 cancers, 96 adenomas, 15 benign mucosa from 14 patients with FAP)...
PVRL4 (or nectin‑4) is a promising therapeutic target since its upregulated expression found in wide range of human cancer types. Enfortumab vedotin, an antibody‑drug conjugate targeting PVRL4, clinically used for the treatment urothelial bladder cancer. In addition, rMV‑SLAMblind, genetically engineered oncolytic measles virus, can infect cells and induce apoptosis through interaction with PVRL4. Although <em>PVRL4</em> transcript levels are elevated breast, lung ovarian cancer, mechanisms...
Familial adenomatous polyposis (FAP) is an inherited disorder characterized by numerous colorectal polyps with predisposition to the development of cancer (CRC). Here, we conducted genome-wide DNA methylation analysis FAP neoplasms, including seven samples and 16 adenoma samples, using Infinium 450K BeadArray. As controls for sporadic neoplasms mucosae, used 450k data from 297 CRC 45 37 normal mucosa reference The Cancer Genome Atlas other databases. Unsupervised two-way hierarchical...
Abstract Aberrant activation of the Wnt/β-catenin signaling pathway plays a crucial role in wide range human tumors including colorectal and liver cancer. The activated causes nuclear accumulation β-catenin, which leads to elevated expression target genes TCF/LEF (T-cell factor/lymphoid enhancer factor) transcription factors through their interaction with β-catenin. Recent studies have identified lot induced by this such as MYC, CCND1, AXIN2, LGR5, clarified roles tumorigenesis. However,...
Abstract Modifications of histone tails control chromatin structure, and the acetylation is generally associated with euchromatin transcriptional activation. Acetylated lysine residues are recognized by bromodomains, which serve as acetyl-lysine binding modules participate in regulation. Bromodomain-containing protein 8 (BRD8) a subunit NuA4/TIP60-histone acetyltransferase complex. Although BRD8 has been considered to act co-activator complex, its biological role remains be unclear. In this...
Pseudomyxoma peritonei (PMP) is a rare disease caused by mucin-producing tumors that develop most frequently from the appendix. The characterized accumulation of mucin in abdominal cavity. Although frequent mutations KRAS and GNAS genes have been reported PMP, gene expression profiles remain to be fully clarified because its rarity difficulties collecting pure cancerous cells scattered within mucin. To disclose molecular features PMP cells, we performed RNA-seq analysis ten PMPs their...
The Wnt/β-catenin signaling pathway plays a key role in development and carcinogenesis. Although some target genes of this have been identified various tissues neoplasms, the comprehensive understanding their roles human cancer, including hepatoma colorectal cancer remain to be fully elucidated. In study, we searched for regulated by Wnt liver using HuH-7 cells. A comparison expression profiles between cells expressing an active form mutant β-catenin enhanced green fluorescent protein (EGFP)...