A5046407838- Chronic Kidney Disease and Diabetes
- Pancreatitis Pathology and Treatment
- Metabolism and Genetic Disorders
- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- Biochemical and Molecular Research
- Pancreatic and Hepatic Oncology Research
- Connective Tissue Growth Factor Research
- Esophageal and GI Pathology
- Hepatocellular Carcinoma Treatment and Prognosis
- Gastroesophageal reflux and treatments
- TGF-β signaling in diseases
- Gallbladder and Bile Duct Disorders
- Renal Diseases and Glomerulopathies
- Heavy metals in environment
- Pancreatic function and diabetes
- Neurotransmitter Receptor Influence on Behavior
- Organ Transplantation Techniques and Outcomes
- Antiplatelet Therapy and Cardiovascular Diseases
- Advanced Glycation End Products research
- Gastrointestinal disorders and treatments
- Nitric Oxide and Endothelin Effects
- Gastrointestinal Bleeding Diagnosis and Treatment
- Cardiac Ischemia and Reperfusion
- Biomedical Research and Pathophysiology
Mayo Clinic in Florida
2021-2025
Jacksonville College
2021-2025
University of Washington
2024-2025
Baystate Medical Center
2023-2024
WinnMed
2021-2024
Johns Hopkins University
2012-2023
Community Medical Center
2023
St. Luke's Hospital
2023
RELX Group (Netherlands)
2023
Johns Hopkins Medicine
2012-2022
Abstract. Connective tissue growth factor (CTGF) is a peptide secreted by cultured endothelial cells and fibroblasts when stimulated transforming factor-β (TGF-β), overexpressed during fibrotic processes in coronary arteries skin. To determine whether CTGF implicated the pathogenesis of diabetic glomerulosclerosis, rat mesangial (MC) as well kidney cortex microdissected glomeruli were examined from obese, db/db mice their normal counterparts. Exposure MC to recombinant human significantly...
To define the interplay of glomerular hypertension and hypertrophy with mesangial extracellular matrix (ECM) deposition, we examined effects capillary distention cell stretching on ECM synthesis. The volume microdissected rat glomeruli (Vg), perfused ex vivo at increasing flows, was quantified related to proximal intraglomerular pressure (PIP). Glomerular compliance, expressed as slope positive linear relationship between PIP Vg 7.68 x 10(3) microns 3/mmHg. Total increment (PIP 0-150 mmHg)...
An environment of high glucose concentration stimulates the synthesis extracellular matrix (ECM) in mesangial cell (MC) cultures. This may result from a similar increase intracellular concentration. We theorized that increased uptake, rather than per se is major determinant exaggerated ECM formation. To test this, we compared effects 35 mM on normal MCs with those 8 same cells overexpressing transporter GLUT1 (MCGT1). Increasing medium to caused total collagen and catabolism, net 81-90%...
Four hundred four patients with chronic Chagas' disease were treated itraconazole (6 mg/kg of body weight/day for 120 days), allopurinol (8.5 60 or a placebo pure starch. Patients monitored over period years by clinical examination, serology, xenodiagnosis, hemoculture, and electrocardiogram. Drug tolerance was good, only treatments discontinued due to side effects that subsided after suspension treatment. Parasitologic cure evident in 44% the those 53% itraconazole, electrocardiographic...
Mesangial matrix deposition is the hallmark of hypertensive and diabetic glomerulopathy. At similar levels systemic hypertension, Dahl salt-sensitive but not spontaneously rats (SHR) develop glomerular which accompanied by upregulation transforming growth factor beta1 (TGF-beta1), mesangial expansion, sclerosis. GLUT-1 ubiquitously expressed predominant glucose transporter in cells. In cells vitro, overexpression increases basal transport, resulting excess fibronectin collagen production....
Cultured mesangial cells (MC) exposed to cyclic mechanical strain or high glucose levels increase their secretion of transforming growth factor-beta1 (TGF-beta1) and collagen, suggesting possible mechanisms for the development diabetic renal sclerosis resulting from intraglomerular hypertension and/or hyperglycemia. This study examines whether interacts with influence collagen metabolism this change is mediated by TGF-beta. Accordingly, rat MC were grown on flexible-bottom plates in 8 35 mM...
TGF-β receptor expression and binding in rat mesangial cells: Modulation by glucose cyclic mechanical strain. <h3>Background</h3> Transforming growth factor-β (TGF-β) is a causal factor experimental glomerulosclerosis, it mediates the increased extracellular matrix (ECM) accumulation that occurs cultured cells (MCs) exposed to high concentrations This change associated with levels of TGF-β, but may also involve alterations binding. <h3>Methods</h3> Rat MCs media containing either 8 or 35 mM...
The effects on renal function of moderate restriction in protein intake were studied 14- to 20-yr-old type I diabetic patients who had no clinical disease or hypertension; matched normal subjects served as controls. After assessment and function, studies conducted at the completion each two consecutive dietary periods 1 wk. Diets containing 3.5 1.5 g X kg-1 day-1 provided during first second periods, respectively. Baseline intakes substantial both controls (1.86 day-1) diabetics (2.17...
To investigate the temporal relationship of diabetes-induced renal growth and its associated metabolic alterations to early development hyperfunction, parallel functional studies were performed shortly after onset diabetes in rats. Hyperglycemia hypoinsulinemia evident 18 h streptozocin injection, significant hyperglucagonemia acidosis present at 36-48 h. Glomerular filtration rate (GFR), expressed per unit body weight, first increased 3 days [1.35 ± 0.07 (SE) (N = 14)] was 18% greater than...
<title>Abstract</title> Purpose Biliary leaks are commonly caused by abdominal trauma, which can be managed endoscopic retrograde cholangiopancreatography (ERCP). The data on factors associated with persistent biliary after successful ERCP is scarce. We aimed to describe the leaks. Methods A retrospective study of an academic level 1 trauma center was conducted patients presenting and had a leak ERCP. primary outcome leak, defined as needing additional excluding stent removal. Results total...
Connective tissue growth factor (CTGF), a member of the closely related CCN family cytokines appears to be fibrotic in skin. To determine whether CTGF is implicated diabetic glomerulosclerosis we studied cultured rat mesangial cells (MC) as well kidney cortex and microdissected glomeruli from obese, db/db mice their normal counterparts. Exposure MC rhCTGF significantly increased fibronectin collagen type I secretion. Further, unstimulated expressed low levels message secreted minimal amounts...