Abstract Conventional cancer treatments can cause serious side effects because they are not specific to cells and damage healthy cells. Aptamers often single-stranded oligonucleotides arranged in a unique architecture, allowing them bind specifically target sites. This feature makes an ideal choice for targeted therapeutics. They typically produced through the systematic evolution of ligands by exponential enrichment (SELEX) undergo extensive pharmacological revision modify their affinity,...

Abstract To explore the proper prognostic markers for likelihood of metastasis in CRC patients. Seventy-seven fresh samples were collected to evaluate mRNA level selected marker using Real-time PCR. Moreover, 648 formalin-fixed paraffin-embedded tissues gathered protein expression by immunohistochemistry (IHC) on tissue microarrays. The results Real-Time PCR showed that low Talin1 was significantly associated with advanced TNM stage ( p = 0.034 ) as well gender 0.029 levels. Similarly, IHC...

DNA damage-inducible transcript 4 (DDIT4) is induced in various cellular stress conditions. This study was conducted to investigate expression and prognostic significance of DDIT4 protein as a biomarker the patients with colorectal cancer (CRC). PPI network KEGG pathway analysis were applied identify hub genes among obtained differentially expressed CRC tissues from three GEO Series. In clinical, one subcellular locations evaluated 198 using immunohistochemistry method on tissue microarrays....

METHODS: Talin-1 protein was demonstrated as a potential prognostic marker in renal cell carcinoma (RCC) using bioinformatics analysis. We, therefore, examined the expression levels and significance of with clinical follow-up total 269 tissue specimens from three important subtypes RCC 30 adjacent normal samples immunohistochemistry. Then, we used combined analysis B7-H3 to investigate higher values. RESULTS: The results showed that high membranous cytoplasmic significantly associated...

Abstract Background Talin-1 as a component of multi-protein adhesion complexes plays role in tumor formation and migration various malignancies. This study investigated protein levels potential prognosis biomarker skin tumors. Methods was evaluated 106 cancer (33 melanomas 73 non-melanomas (NMSC)) 11 normal formalin-fixed paraffin-embedded (FFPE) tissue samples using immunohistochemical technique on microarrays (TMAs). The association between the expression clinicopathological parameters,...

DNA damage-inducible transcript 4 (DDIT4), also known as Redd1, Dig2, and RTP801 was identified to be upregulated in response a variety of cellular stresses, including damage, endoplasmic reticulum stress, energy stress. Several studies have discovered that dysregulation DDIT4 involved various cancers with paradoxical expression roles. Hence, this study designed investigate the clinical significance prognostic value different subtypes gastric cancer (GC). To evaluate pattern GC tissues well...

Nearly half of patients with colorectal cancer (CRC), the third leading cause deaths worldwide, are diagnosed in late stages disease. Appropriate treatment is not applied a timely manner and nearly 90% who experience metastasis ultimately die. Timely detection CRC can increase five-year survival rate patients. Existing histopathological molecular classifications insufficient for prediction metastasis, which limits approaches to treatment. Detection reliable cancer-related biomarkers improve...

Diffuse large B cell lymphoma (DLBCL) is the most common type of non-Hodgkin (NHL) in adults. Although studies regarding association between expression Ki-67, CD10, BCL6, and MUM1 proteins, as well c-MYC amplification EBV status with clinicopathologic characteristics have rapidly progressed, their co-expression prognostic role remain unsatisfactory. Therefore, this study aimed to investigate all markers features value DLBCL. Also, was investigated.

Melanoma antigen gene-A2 (MAGE-A2) is one of the most cancer-testis antigens overexpressed in a variety malignancies. However, expression MAGE-A2 for clinical values pathophysiology renal cell carcinoma (RCC) unknown. For first time, present study was conducted to examine and prognostic significance clear RCC (ccRCC). assayed 162 well-defined ccRCC samples using immunohistochemistry staining on tissue microarrays. The association between clinic-pathologic features as well survival outcomes...

DNA damage-inducible transcript 4 (DDIT4), induced under cellular stress conditions, has been implicated in malignancies due to its abnormal expression patterns. The of DDIT4 at the mRNA level and potential role as a prognostic biomarker gliomas were analyzed using GEPIA tool. To validate these findings, protein levels their significance examined tissue microarrays from glioma patients immunohistochemistry clinical samples. Bioinformatics analysis revealed that overexpression tumors high are...

Aim and Objective: It is interesting to find the gene signatures of cancer stages based on omics data. The aim study was evaluate enrich array data using ontology ncRNA databases in colorectal cancer. Methods: human were obtained from GEO databank. downregulated up-regulated genes identified after scoring, weighing merging clusters with high-score edges determined networks. miRNAs related enriched. Furthermore, long non-coding RNA (lncRNA) networks predicted a central core for miRNAs....

Spalt-like transcription factor 4 (SALL4) and aldehyde dehydrogenase1 family member A1 (ALDH1A1) expressing cells have been characterized as possessing stem cell-like properties known cancer cell marker in serous ovarian carcinoma (SOC).The association between SALL4 ALDH1A1 was observed based on literature review bioinformatics tools. Therefore, this study aimed to investigate the co-expression of SALL4/ALDH1A1 proteins clinicopathological parameters their prognostic value SOC patients using...

Abstract Background Various diagnostic and prognostic tools exist in colorectal cancer (CRC) due to multiple genetic epigenetic alterations causing the disease. Today, expression of RNAs is being used as markers for cancer. Methods In current study, various dysregulated CRC were identified via bioinformatics prediction. Expression several these measured by RT-qPCR 48 tissues from patients well stem cell-enriched spheroids derived HT-29 cell line. The relationships between levels...

Dynamin 2 (DNM2) involved in tumor progression various malignancies.For the first time, we evaluated DNM2 expression pattern, its association with clinicopathological characteristics and survival outcomes RCC subtypes.We pattern tissues as well adjacent normal tissue using immunohistochemistry on microarray (TMA) slides.Our findings revealed increased samples rather than tissues. The results indicated that there was a statistically significant difference between cytoplasmic of among subtypes...

Analysis of circulating tumor DNA (ctDNA) can be used to characterize and monitor cancers. Recently, non-invasive prenatal testing (NIPT) as a new next-generation sequencing (NGS)-based approach has been applied for detecting ctDNA. This study aimed investigate the copy number variations (CNVs) utilizing in plasma ctDNA from ovarian cancer (OC) patients who were treated with neoadjuvant chemotherapy (NAC). The samples six patients, including stages II-IV, collected during pre- post-NAC...

The molecular mechanisms of drug use on sexual health are largely unknown. We investigated, the relationship between heroin disorder and epigenetic factors influencing histone acetylation in sperm cells. volunteers included twenty-four 20- to 50-year-old men with a normal spermogram who did not consume any drugs age- BMI-matched only for more than last four months. HDAC1 HDAC11 mRNA expression levels spermatozoa miR-34c-5p miR-125b-5p seminal plasma were measured. heroin-user group showed...

Abstract Background TWIST1 and CD105, which contribute to tumor malignancy, are overexpressed in cancers. Accordingly, enhances epithelial-to-mesenchymal transition (EMT) promotes the formation of cancer stem cells (CSCs). Also, CD105 is a neoangiogenesis marker endothelial cells, introduced as CSC tumoral epithelial several types The present study was aimed investigate expressions colorectal (CRC) patients. Methods Expressions 250 CRC tissue samples were evaluated using immunohistochemistry...

DNA damage-inducible transcript 4 (DDIT4) is induced in various cellular stress conditions. Several studies showed that the dysregulation of DDIT4 involved different malignancies with paradoxical expressions and roles. Therefore, this study investigated clinical significance, prognostic, diagnostic value types pancreatic tumors (PT). The expression long non-coding RNA (TPTEP1) mRNA level was examined 27 fresh PT samples using Real-time quantitative PCR (RT-qPCR). Moreover, 200 formalin-fixed...