A5085279807- Aquaculture disease management and microbiota
- Reproductive biology and impacts on aquatic species
- Invertebrate Immune Response Mechanisms
- Aquaculture Nutrition and Growth
- SARS-CoV-2 detection and testing
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Environmental Toxicology and Ecotoxicology
- Fish Ecology and Management Studies
- Plant Virus Research Studies
- Nanoparticles: synthesis and applications
- Biosensors and Analytical Detection
- Insect symbiosis and bacterial influences
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Pharmaceutical and Antibiotic Environmental Impacts
- Vibrio bacteria research studies
- Identification and Quantification in Food
- Mosquito-borne diseases and control
- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- Parasite Biology and Host Interactions
- Marine Bivalve and Aquaculture Studies
- Physiological and biochemical adaptations
- Viral Infections and Vectors
- Heavy Metal Exposure and Toxicity
Centre for Environment, Fisheries and Aquaculture Science
2016-2025
University of Exeter
2010-2025
UK Health Security Agency
2022-2023
King's College London
2013
University of Birmingham
2009-2010
Brunel University of London
1999-2001
Steroidal estrogens, originating principally from human excretion, are likely to play a major role in causing widespread endocrine disruption wild populations of the roach (Rutilus rutilus), common cyprinid fish, rivers contaminated by treated sewage effluents. Given extent this problem, risk assessment models needed predict location and severity river catchments identify areas where regulation discharges remove these contaminants is necessary. In study we attempted correlate British rivers,...
Despite increasing application of silver nanoparticles (NPs) in industry and consumer products, there is still little known about their potential toxicity, particularly to organisms aquatic environments. To investigate the fate effects NPs fish, rainbow trout (Oncorhynchus mykiss) were exposed via water commercial particles three nominal sizes: 10 nm (N10), 35 (N35), 600–1600 (NBulk), nitrate for days. Uptake into gills, liver, kidneys was quantified by inductively coupled plasma-optical...
Nanoparticles (NPs) are reported to be a potential environmental health hazard. For organisms living in the aquatic environment, there is uncertainty on exposure because of lack understanding and data regarding fate, behavior, bioavailability nanomaterials water column. This paper reports series integrative biological physicochemical studies uptake unmodified commercial nanoscale metal oxides, zinc oxide (ZnO), cerium dioxide (CeO(2)), titanium (TiO(2)), from diet determine their...
Silver nanoparticles cause toxicity in exposed organisms and are an environmental health concern. The mechanisms of silver nanoparticle toxicity, however, remain unclear. We examined the effects exposure to nano-, bulk-, ionic forms on zebrafish embryos (Danio rerio) using a Next Generation Sequencing approach Illumina platform (High-Throughput SuperSAGE). Significant alterations gene expression were found for all treatments many pathways affected, most notably those associated with...
Abstract An enzyme-linked immunosorbent assay (ELISA) based oncarp (Cyprinus carpio) vitellogenin (c-VTG, an estrogen-dependent precursor of egg yolk) was developed to quantify VTG in the fathead minnow (Pimephales promelas) and employed establish vivo testing system for estrogens fish early life stages. The working range c-VTG ELISA between 1 50 ng/ml (20%–80% binding). inter- intraassay variations were 9.7 ± 3% 8.9 3.4% (n = 15), respectively. Plasma whole body homogenates from females...
To establish whether the intersex condition seen in roach (Rutilus rutilus) United Kingdom (UK) rivers was species specific or a more general phenomenon fish, evidence for sexual disruption sought second cyprinid species, gudgeon (Gobio gobio). Gudgeon were collected from Rivers Aire and Lea (at locations that receive high-volume discharges of sewage treatment works [STW] effluent contain roach) two still waters, their gonads examined histologically intersexuality (the simultaneous presence...
The mechanisms underlying the initiation of puberty in fish are poorly understood, and whether Kiss1 receptor (Kiss1r; previously designated G protein-coupled 54; GPR54) its ligands, kisspeptins, play a significant role, as has been established mammals, is not yet known. We determined (via real-time PCR) temporal patterns expression brain kiss1r, gnrh2, gnrh3 suite related genes hypothalamo-pituitary-gonadal (HPG) axis analyzed them against timing gonadal germ cell development male female...
Copper (Cu) is a micronutrient essential for the biochemical functioning of numerous processes in vertebrates but also often present aquatic environment at concentrations able to cause adverse health effects organisms. This study investigated signaling pathways mediating exposure Cu using toxicogenomic approach fish model, stickleback (Gasterosteus aculeatus). Freshwater-acclimated male were exposed via water Cu, including environmentally relevant (3.2−128 μg Cu/L 4 days), and biological...
Bisphenol A (BPA) is a commercially important high production chemical widely used in epoxy resins and polycarbonate plastics, ubiquitous the environment. Previous studies demonstrated that BPA activates estrogenic signaling pathways associated with adverse effects on reproduction vertebrates exposure can induce epigenetic changes. We aimed to investigate reproductive of fish model document its mechanisms toxicity. exposed breeding groups zebrafish (Danio rerio) 0.01, 0.1, 1 mg/L for 15 d....
Although Astyanax mexicanus surface fish regenerate their hearts after injury, Pachón cave-dwelling counterparts cannot and, instead, form a permanent fibrotic scar, similar to the human heart. Myocardial proliferation peaks at levels in both and 1 week injury. However, Pachón, this peak coincides with strong scarring immune response, ultimately, cavefish cardiomyocytes fail replace scar. We identified lrrc10 be upregulated compared Similar cavefish, knockout of zebrafish impairs heart...
Most public health initiatives that monitor viruses in wastewater have utilized quantitative polymerase chain reaction (PCR) and whole genome PCR sequencing, mirroring techniques used for viral epidemiology individuals. These require prior knowledge of the target are limited to monitoring individual or small groups viruses. Metagenomic sequencing may offer an alternative strategy a broad spectrum wastewater, including novel emerging pathogens. In this study, while amplicon gave high...
Our recent work suggests limited uptake of unstabilized metal oxide nanoparticles via water into fish, however, some other studies have indicated such exposures can induce oxidative stress. To investigate tissue distribution and toxicity titanium dioxide (TiO2) that may enter we conducted a series injection studies. Rainbow trout (Oncorhynchus mykiss) were intravenously injected with 100 μg TiO2 the content in blood, brain, gills, liver, kidney quantified at time points between 6 h 90 days...
Worldwide, a number of viable populations fish are found in environments heavily contaminated with metals, including brown trout (Salmo trutta) inhabiting the River Hayle South-West England. This population is chronically exposed to water-borne mixture copper and zinc, at concentrations lethal naïve fish. We aimed investigate molecular mechanisms employed by tolerate high metal concentrations. To achieve this, we combined tissue analysis whole-transcriptome profiling using RNA-seq on an...
Panulirus argus virus 1 (PaV1) is the only known infecting Caribbean spiny lobster (Panulirus argus) from Sea. Recently, related viruses, Dikerogammarus haemobaphes (DhV1) and Carcinus maenas (CmV1), have been detected in demon shrimp (Dikerogammarus haemobaphes) European shore crab (Carcinus maenas), respectively, sites United Kingdom. The virion morphology of these crustacean viruses similar to that iridoviruses. However, unlike iridoviruses other nucleocytoplasmic large DNA (NCLDVs),...