A5082616191- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- Drug-Induced Hepatotoxicity and Protection
- Lipid metabolism and biosynthesis
- Adipokines, Inflammation, and Metabolic Diseases
- Diet, Metabolism, and Disease
- Metabolism and Genetic Disorders
- Fatty Acid Research and Health
- Inflammasome and immune disorders
- Diabetes and associated disorders
- Adipose Tissue and Metabolism
- Immune Response and Inflammation
- Protein Interaction Studies and Fluorescence Analysis
- Colorectal Cancer Screening and Detection
- Organ Transplantation Techniques and Outcomes
- Proteoglycans and glycosaminoglycans research
- Cytokine Signaling Pathways and Interactions
- Pancreatic function and diabetes
- Macrophage Migration Inhibitory Factor
- Alcohol Consumption and Health Effects
- Chronic Lymphocytic Leukemia Research
- Endoplasmic Reticulum Stress and Disease
- Monoclonal and Polyclonal Antibodies Research
- Clinical Nutrition and Gastroenterology
- Erythrocyte Function and Pathophysiology
Hospital Clínic de Barcelona
2017-2024
European Foundation for the Study of Chronic Liver Failure
2020-2024
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2017-2024
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2019-2024
Grifols (Spain)
2021-2024
Universitat de Barcelona
2019
Background & AimsWe investigated the effect of albumin treatment (20% solution) on hypoalbuminemia, cardiocirculatory dysfunction, portal hypertension, and systemic inflammation in patients with decompensated cirrhosis without bacterial infections.MethodsWe performed a prospective study to assess effects long-term (12 weeks) low doses (1 g/kg body weight every 2 high (1.5 week) serum albumin, plasma renin, function, pressure, levels cytokines, collecting data from 18 infections (the...
Decompensated cirrhosis is characterized by exuberant systemic inflammation. Although the inducers of this feature remain unknown, presence circulating forms oxidized albumin, namely human nonmercaptalbumin 1 (HNA1) and HNA2, a common finding in cirrhosis. The aim study was to explore ability these albumin induce inflammation triggering activation peripheral leukocytes. We observed significantly higher plasma levels HNA1 HNA2 patients with (n = 256) compared healthy volunteers 48), which...
Patients with acute-on-chronic liver failure (ACLF) present a systemic hyperinflammatory response associated increased circulating levels of small-molecule metabolites. To investigate whether these alterations reflect inadequate cell energy output, we assessed mitochondrial morphology and central metabolic pathways emphasis on the tricarboxylic acid (TCA) cycle in peripheral leukocytes from patients acutely decompensated (AD) cirrhosis, without ACLF.The study included samples AD cirrhosis...
Human serum albumin (HSA) is an emerging treatment for preventing excessive systemic inflammation and organ failure(s) in patients with acutely decompensated (AD) cirrhosis. Here, we investigated the molecular mechanisms underlying immunomodulatory properties of HSA. Administration HSA to AD cirrhosis elevated circulating bacterial DNA rich unmethylated cytosine-phosphate-guanine dideoxynucleotide motifs (CpG-DNA) was associated reduced plasma cytokine concentrations. In isolated leukocytes,...
Specialized proresolving mediators (SPMs) biosynthesized from docosahexaenoic acids (DHAs) including resolvins (Rvs), protectins, and maresins are potent endogenous autacoids that actively resolve inflammation, protect organs, stimulate tissue regeneration. Our hypothesis was failure of resolution programs may lead to unremitting inflammation in obesity, contributing the development metabolic comorbidities this condition. Obese individuals with persistent low-grade systemic showed reduced...
Endoplasmic reticulum (ER) stress and activation of the unfolded protein response (UPR) are hallmarks non-alcoholic fatty liver disease (NAFLD), which is hepatic manifestation metabolic syndrome associated with obesity.The specialized pro-resolving lipid mediator maresin 1 (MaR1) preserves tissue homeostasis by exerting cytoprotective actions, dampening inflammation expediting its timely resolution.Here, we explored whether MaR1 protects cells from lipotoxic hypoxia-induced ER stress.Mice...
Non-alcoholic steatohepatitis (NASH) is the hepatic manifestation of metabolic syndrome and characterized by steatosis, inflammation fibrosis. Soluble guanylate cyclase (sGC) stimulation reduces fibrosis in experimental models lung, kidney heart disease. Here, we tested whether sGC also effective NASH.NASH was induced mice feeding a choline-deficient, l-amino acid-defined, high-fat diet. These received either placebo or stimulator IW-1973 at two different doses (1 3 mg·kg-1 ·day-1 ) for 9...
Significance Fatty liver, which is an initial step in the development of more severe complications such as liver cirrhosis, prevalent worldwide our society. This study demonstrates that stimulation soluble guanylate cyclase (sGC), enzyme producing second messenger cGMP, protects against most common features fatty namely inflammation and fibrosis, animal models disease. Our also provides explanation for this protection describes how sGC blocks inflammasome (a protein complex responsible...
Besides its oncotic power, albumin exerts pleiotropic actions, including binding, transport, and detoxification of endogenous exogenous molecules, antioxidant activity, modulation immune inflammatory responses. In particular, recent studies have demonstrated that reduces leukocyte cytokine production. Here, we investigated whether also has the ability to protect tissues from damaging actions these mediators. We circumscribed our investigation tumor necrosis factor (TNF) α, which exemplifies...
Abstract Background and aim Extracellular matrix (ECM) components released during excessive fat mass expansion are considered potential endogenous danger/alarm signals contributing to innate immune system activation. The of the current study was specifically measure plasma levels low molecular weight (LMW) hyaluronan (HA) evaluate its role as pro-inflammatory damage-associated pattern (DAMP) on leukocyte response in context human obesity. Subjects methods Participants were selected according...
Background and Aim: Injury to hepatocyte mitochondria is common in metabolic dysfunction‐associated fatty liver disease. Here, we investigated whether changes the content of essential acid–derived lipid autacoids affect mitochondrial bioenergetics efficiency. Approach Results: The study was performed transgenic mice for fat‐1 gene, which allows endogenous replacement membrane omega‐6–polyunsaturated acid (PUFA) composition by omega‐3–PUFA. Transmission electron microscopy revealed that had...
Abstract Cytokine‐induced inflammation and mitochondrial oxidative stress are key drivers of liver tissue injury. Here, we describe experiments modeling hepatic inflammatory conditions in which plasma leakage leads to large amounts albumin reach the interstitium parenchymal surfaces explore whether this protein plays a role preserving hepatocyte mitochondria against damaging actions cytotoxic cytokine tumor necrosis factor alpha (TNFα). Hepatocytes precision‐cut slices were cultured absence...
Obesity comorbidities are closely associated with chronic low-grade adipose tissue inflammation. A number of SNPs inflammation has been identified, underscoring the impact genetic determinants on this process. Here, we screened in genes pro-inflammatory (IL-1β, IL-6, STAT3 and JAK2), anti-inflammatory (IL-10 SOCS3) pro-resolving (ERV1/ChemR23) properties 101 obese 99 non-obese individuals. Among analyzed, identified that individuals carrying a C allele rs1878022 polymorphism ERV1/ChemR23...
Highlights•Palmitoylcarnitine is elevated in plasma of patients with acute decompensation cirrhosis.•This long-chain acylcarnitine induces mitochondrial oxidative stress leukocytes.•It suppresses heme oxygenase 1 and the secretion proinflammatory cytokines.•These effects were prevented by blocking entry palmitoylcarnitine its oxidation mitochondria.AbstractBackground & AimsIn cirrhosis, (AD) correlates a hyperinflammatory state driven dysfunction, which significant factor progression to...
Albumin infusions are therapeutically used to revert hypoalbuminemia and replace the extensively oxidized albumin molecule circulating in patients with acutely decompensated (AD) cirrhosis. Because has high affinity for lipids, here we characterized lipidome AD explored effects on release of fatty acid (FA)–derived lipid mediators by peripheral leukocytes. Lipids were measured liquid chromatography–tandem mass spectrometry albumin‐enriched albumin‐depleted plasma fractions separated...
Metabolic dysfunction-associated Steatohepatitis (MASH), is a prominent cause for liver cirrhosis. MASH-cirrhosis responsible complications and there no specific treatment. To develop new therapeutic approaches, animal models are needed. The aim of this study was to fast model in rats reflecting the human disease. Carbon tetrachloride (CCl