A5050061139- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Immune Response and Inflammation
- CAR-T cell therapy research
- Bone and Dental Protein Studies
- Glycosylation and Glycoproteins Research
- Virus-based gene therapy research
- Reproductive System and Pregnancy
- Cytokine Signaling Pathways and Interactions
- Immunodeficiency and Autoimmune Disorders
- RNA Interference and Gene Delivery
- Diabetes and associated disorders
- Galectins and Cancer Biology
- Herpesvirus Infections and Treatments
- Cancer Immunotherapy and Biomarkers
- Lymphoma Diagnosis and Treatment
- Artificial Immune Systems Applications
- T-cell and Retrovirus Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Cell Adhesion Molecules Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Atherosclerosis and Cardiovascular Diseases
- Mast cells and histamine
Rutgers, The State University of New Jersey
2024
Dana-Farber Cancer Institute
2014-2023
Harvard University
2014-2023
Memorial Sloan Kettering Cancer Center
1975-2015
Stanford University
1973-2015
Hôpital Européen Georges-Pompidou
2015
Université Paris Cité
2015
Assistance Publique – Hôpitaux de Paris
2015
Inserm
2015
Heinrich Heine University Düsseldorf
2014
Cell-mediated (type-1) immunity is necessary for immune protection against most intracellular pathogens and, when excessive, can mediate organ-specific autoimmune destruction. Mice deficient in Eta-1 (also called osteopontin) gene expression have severely impaired type-1 to viral infection [herpes simplex virus–type 1 (KOS strain)] and bacterial ( Listeria monocytogenes ) do not develop sarcoid-type granulomas. Interleukin-12 (IL-12) interferon-γ production diminished, IL-10 increased. A...
Ly alloantigens coded by two unlinked genetic loci (Ly-1 and Ly-2/Ly-3) are expressed on lymphoid cells undergoing thymus-dependent differentiation. Peripheral Thy-1+ from C57BL/6 mice can be divided into three subclasses the basis of differential expression Ly-1, Ly-2, Ly-3; about 50% express all antigens (Ly -123+), 33% only Ly-1 (Ly-1+), 6-8% Ly-2 Ly-3 (Ly-23+). Cells Ly-123+ first peripheral to appear in ontogeny, reduced periphery shortly after adult thymectomy. In contrast, Ly-1+...
The CD44 family of surface receptors regulates adhesion, movement, and activation normal neoplastic cells. cytokine osteopontin (Eta-1), which similar cellular functions, was found to be a protein ligand CD44. Osteopontin induces chemotaxis but not homotypic aggregation, whereas the inverse is true for interaction between carbohydrate ligand, hyaluronate. different responses cells after ligation by either or hyaluronate may account independent effects on cell migration growth. This mechanism...
Lymphocytes from BALB/c or C57B6/6 mice that develop killer activity to alloantigens belong the numerically small Ly-23 subclass of peripheral T cells, distinguished by selective expression determinants on their surfaces. The maturation these cells can be amplified Ly-1+ which do not themselves contribute cell pool. This amplification was abolished escluding Ia("Beta")+ stimulator population during mixed lymphocyte culture (MCL), suggesting is due recognition I region antigens L-1+ a...
Viral infection is sometimes associated with the initiation or exacerbation of autoimmune disease, although underlying mechanisms remain unclear. One proposed mechanism that viral determinants mimic host antigens trigger self-reactive T cell clones to destroy tissue. An epitope expressed by a coat protein herpes simplex virus–type 1 (HSV-1) KOS strain has now been shown be recognized autoreactive cells target corneal in murine model stromal keratitis. Mutant HSV-1 viruses lacked this did not...
T helper type 17 (TH17) cells are highly proinflammatory effector that characterized by the production of high amounts IL-17A, IL-17F, IL-21, and IL-22. Furthermore, TH17 have been associated with a number autoimmune diseases. However, it is not clear whether can also serve as effective cells. Here we show function B-cell helpers in they only induce strong proliferative response B vitro but trigger antibody class switch recombination vivo. Transfer into WT or T-cell receptor α–deficient...
These experiments test the hypothesis that cells carrying Ly1+23- surface phenotype are programmed exclusively for helper and not suppressive activity regardless of external conditions such as mode or type antigen stimulation. To this end, we have stimulated purified populations Ly1 with in vitro using devised to induce unselected T express optimal levels specific T-suppressor activity. We find after stimulation, generate SRBC-specific T-helper but T-suppressive findings establish...
How T cells maintain their identity Although best known for pathogen-fighting prowess, lymphocytes also ensure that the immune response does not run amok. A subset of called regulatory (T regs ) performs this function by, example, making sure only attack pathogens and self. can exhibit plasticity in functions face an inflammatory stimulus. Kim et al. sought to identify molecules stable maintenance . Using genetically modified mice, they found both CD4 + CD8 require transcription factor...
It has become increasingly clear that both the type and intensity of immune response to antigen are controlled by a complex series interactions among different types T lymphocytes macrophages. This conclusion comes from several lines experiments. First, studies cellular basis have indicated cells capable helper-initiator, suppressive, cytolytic effects, these activities specialized functions distinct subclasses (Shiku et al. 1975; Cantor Boyse 1975a,b; 1976; Jandinski Huber 1976). Studies...
A cDNA sequence coding for mouse mast-cell growth-factor (MCGF) has been cloned from a library prepared mRNA derived concanavalin A-activated T-cell clone. Cloning was achieved using the pcD vector that permits expression of inserts in mammalian cells. The DNA codes polypeptide 166 amino acid residues including putative signal peptide. supernatant fluid obtained after transfection COS-7 monkey cells with pcD-MCGF plasmid had MCGF activity. between acids 33 and 41, deduced nucleotide its...
Cells of the Lyl subclass generate helper activity in both primary and secondary responses to sheep erythrocytes (SRBC). In contrast, after priming with SRBC, cells Ly-2+ subclasses, particular Ly23 cells, have suppressive activity. The degree Ly23-mediated suppression is directly proportional amount antigen (SRBC) used for priming. Suppression by specific, because from SRBC-primed animals do not suppress response horse erythrocytes, vice versa. Thus, cytotoxic specific suppressor functions...
We have shown that (a) purified T-helper cells induce of another T-cell set-, expressing the Ly123+Qa1+ surface phenotype, to exert potent suppressive activity, (b) this T-T interaction plays an important role in regulating vivo immune responses, and (c) represents barrier protocols intended augment status individuals by adoptive (or active) immunotherapy. Our results also indicate Ly123+ set mediating feedback suppression is sensitive both low doses cyclophosphamide removal thymus adult...
Concanavalin A, a nonspecific polyclonal activator of T lymphocytes, activates Lyl and Ly23 subclasses to the same degree. After activation, subclass, but not has following properties: (a) Suppression antibody response sheep erythrocytes (SRBC) in vitro. (b) Production soluble factor that suppresses anti-SRBC (c) generation cell-mediated cytotoxicity H-2 target cells Con A-activated express helper function Because intact A-stimulated T-cell population contains both cell types, these do exert...
Although there is considerable evidence that a subpopulation of regulatory CD4 + CD25 T cells can suppress the response autoreactive cells, underlying molecular mechanism not understood. We find transmission suppressive signal by CD4CD25 requires engagement B7 molecule expressed on target cells. The from B7-deficient mice resistant to suppression in vitro , and these provoke lethal wasting disease lymphopenic despite presence Susceptibility restored lentiviral-based expression full-length,...
Abstract We have tested the proposition that induction of certain sets B cell clones to produce antibody requires a signal from T helper cells recognize idiotypic determinants expressed on Ig receptors relevant clones. The approach is based analysis populations required induce secrete anti‐arsonate antibodies are marked by cross‐reactive idiotype (CRId). CRId + anti‐azophenyl arsonate (Ar) produced in A/J strain mice after immunization with Ar keyhole limpet hemocyanin and represent 20–70%...
Neutrophil-independent macrophage responses are a prominent part of delayed-type immune and healing processes depend on T cell-secreted cytokines. An important mediator in this setting is the phosphoprotein osteopontin, whose secretion by activated cells confers resistance to infection several intracellular pathogens through recruitment activation macrophages. Here, we analyze structural basis activity following cleavage thrombin into two fragments. interaction between C-terminal domain...