A5066833720- Hedgehog Signaling Pathway Studies
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Blood properties and coagulation
- Pancreatic and Hepatic Oncology Research
- Tumors and Oncological Cases
- Genetic and rare skin diseases.
- Cancer and Skin Lesions
- Oral and Maxillofacial Pathology
- Cancer Cells and Metastasis
- Cholangiocarcinoma and Gallbladder Cancer Studies
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- DNA Repair Mechanisms
- Nonmelanoma Skin Cancer Studies
- Cancer Mechanisms and Therapy
- Cancer, Lipids, and Metabolism
- Cholesterol and Lipid Metabolism
- Lung Cancer Treatments and Mutations
- Cleft Lip and Palate Research
- Melanoma and MAPK Pathways
- Ethics and Legal Issues in Pediatric Healthcare
- Hippo pathway signaling and YAP/TAZ
- Genital Health and Disease
- Immune cells in cancer
Indiana University – Purdue University Indianapolis
2013-2023
Southern Medical University
2023
Center for Scientific Review
2023
National Institutes of Health
2023
Zhujiang Hospital
2023
Indiana University School of Medicine
2012-2022
Indiana University
2009-2022
University School
2011-2022
Indiana University Health
2010-2021
Center for Children
2014-2016
The basal cell nevus syndrome (BCNS) is characterized by developmental abnormalities and the postnatal occurrence of cancers, especially carcinomas (BCCs), most common human cancer. Heritable mutations in BCNS patients a somatic mutation sporadic BCC were identified homolog Drosophila patched (ptc) gene. ptc gene encodes transmembrane protein that acts opposition to Hedgehog signaling protein, controlling fates, patterning, growth numerous tissues. PTC appears be crucial for proper embryonic...
The hedgehog pathway plays a critical role in the development of prostate. However, prostate cancer is not clear. Prostate second most prevalent cause death American men. Therefore, identification novel therapeutic targets for has significant clinical implications. Here we report that activation occurs frequently advanced human cancer. We find high levels target genes, PTCH1 and hedgehog-interacting protein (HIP), are detected over 70% tumors with Gleason scores 8–10, but only 22% 3–6....
Cancer cells are known to execute reprogramed metabolism of glucose, amino acids and lipids. Here, we report a significant role cholesterol in cancer metastasis. By using label-free Raman spectromicroscopy, found an aberrant accumulation cholesteryl ester human pancreatic specimens cell lines, mediated by acyl-CoA acyltransferase-1 (ACAT-1) enzyme. Expression ACAT-1 showed correlation with poor patient survival. Abrogation esterification, either inhibitor or shRNA knockdown, significantly...
Hedgehog (Hh) signaling is deregulated in multiple human cancers including pancreatic ductal adenocarcinoma (PDA). Because KRAS mutation represents one of the earliest genetic alterations and occurs almost universally PDA, we hypothesized that oncogenic promotes tumorigenesis part through activation Hh pathway. Here, report activates hedgehog PDA cells, utilizing a downstream effector pathway mediated by RAF/MEK/MAPK but not phosphatidylinositol 3-kinase (PI3K)/AKT. Oncogenic transformation...
Liver cancers, the majority of which are hepatocellular carcinomas (HCCs), rank as fourth in cancer mortality worldwide and most rapidly increasing type United States. However, molecular mechanisms underlying HCC development not well understood. Activation hedgehog pathway is shown to be involved several types gastrointestinal cancers. Here, we provide evidence indicate that signaling activation occurs frequently HCC. We detect expression Shh , PTCH1 Gli1 115 cases 44 liver tissues adjacent...
Activation of the hedgehog pathway, through loss patched (PTC) or activation smoothened (SMO), occurs frequently in basal cell carcinoma (BCC), most common human cancer. However, molecular basis this neoplastic effect is not understood. The downstream molecule Gli1 known to mediate biological pathway and itself up-regulated all BCCs. can drive production BCCs mouse when overexpressed epidermis. Here we show that activate platelet-derived growth factor receptor α (PDGFRα) C3H10T½ cells....
The hedgehog pathway plays a critical role in the development of foregut. Recent studies indicate that activation occurs stomach and other gastrointestinal cancers. However, association with tumor stage, differentiation subtype is not well documented. Here, we report our findings elevated expression target genes human patched gene 1 (PTCH1) or Gli1 63 99 primary gastric Activation associated poorly differentiated more aggressive tumors. sonic (Shh) transcript localized to cancer tissue,...
The fifth national consensus conference on the management of Helicobacter pylori (H.pylori) infection organized by Chinese Medical Association, Society Gastroenterology, H. and Peptic Ulcer Study Group was held at Hangzhou, Zhejiang Province December 15-16, 2016.More than 80 members study group experts in field research gastroenterology attended meeting.Consensus preparatory established to draft related statements.The quality evidence strength recommendation were evaluated GRADE system.The...
Abnormal activation of the hedgehog-signaling pathway is pivotal abnormality driving growth basal cell carcinomas (BCCs), most common type human cancer. Antagonists this such as cyclopamine may therefore be useful for treatment and other hedgehog-driven tumors. We report here that chronic oral administration dramatically reduces ( approximately 66%) UVB induced carcinoma formation in Ptch1(+/-) mice. Fas expression low murine but up-regulated presence smoothened (SMO) antagonist,...
The hedgehog pathway plays a critical role in the development of foregut. However, primary esophageal cancers is not well studied. Here, we report that elevated expression target genes occurs 14 22 cancers. signaling activation associated with tumor subtypes, stages, or differentiation. While sonic (Shh) transcript localized to tissue, Gli1 and PTCH1 observed both stroma. We discovered 4 squamous cell carcinomas, which overexpress Shh, have genomic amplification Shh gene. Treatment cancer...
The hedgehog (Hh) signaling pathway is essential for embryonic development and carcinogenesis. Activation of Hh has been identified in several types gastrointestinal cancers, including esophageal, gastric, pancreatic, liver cancers. Several recent studies suggest that activation can inhibit Wnt signaling. However, the molecular basis underlying this inhibition remains unclear. As transcription factors pathway, Gli molecules transform cells culture, their expression are associated with cancer...
The protooncogene MYC plays an important role in the regulation of cellular proliferation, differentiation, and apoptosis has been implicated a variety human tumors. closely related MYCN encode highly conserved nuclear phosphoproteins (Myc NMyc) that apparently function as transcription factors cell. We have identified large protein interacts directly with transcriptional activating domain Myc (designated “protein associated Myc” or Pam). Pam contains extended amino acid sequence...
Human AP-endonuclease (APE1/Ref-1), a central enzyme involved in the repair of oxidative base damage and DNA strand breaks, has second activity as transcriptional regulator that binds to several trans-acting factors. APE1 overexpression is often observed tumor cells confers resistance various anticancer drugs; its downregulation sensitizes such agents. Because involvement repairing induced by many these drugs unlikely, drug may be linked APE1's regulatory function. Here, we show APE1,...
The hedgehog (Hh) pathway plays a critical role during development of embryos and cancer. Although the molecular basis by which protein kinase A (PKA) regulates stability downstream transcription factor cubitus interruptus, Drosophila homologue vertebrate Gli molecules, is well documented, mechanism PKA inhibits functions molecules in vertebrates remains elusive. Here, we report that activation retains Gli1 cytoplasm. Conversely, inhibition activity promotes nuclear accumulation Gli1....
Abstract Purpose: Aggressive pancreatic cancer is commonly associated with a dense desmoplastic stroma, which forms protective niche for cells. The objective of the study was to determine functions tissue transglutaminase (TG2), Ca2+-dependent enzyme that cross-links proteins through transamidation and abundantly expressed by cells in stroma. Experimental Design: Orthotopic xenografts coculture systems tested mechanisms modulates tumor–stroma interactions. Results: We show TG2 secreted...
Pancreatic cancer, mostly pancreatic ductal adenocarcinomas (PDAC), is one of the most lethal cancers, with a dismal median survival around 8 months. PDAC notoriously resistant to chemotherapy. Thus far, numerous attempts using novel targeted therapies and immunotherapies yielded limited clinical benefits for cancer patients. It hoped that delineating molecular mechanisms underlying drug resistance in may provide therapeutic options. Using acquired gemcitabine cell lines, we revealed an...