A5024128481- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Immunodeficiency and Autoimmune Disorders
- Animal Virus Infections Studies
- Cytomegalovirus and herpesvirus research
- Inflammasome and immune disorders
- Kawasaki Disease and Coronary Complications
- Autoimmune and Inflammatory Disorders Research
- Parvovirus B19 Infection Studies
- Immune Cell Function and Interaction
- Herpesvirus Infections and Treatments
- vaccines and immunoinformatics approaches
- Respiratory viral infections research
- COVID-19 epidemiological studies
- Immune responses and vaccinations
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- Viral gastroenteritis research and epidemiology
- Blood groups and transfusion
Research Institute Hospital 12 de Octubre
2020-2023
Instituto de Salud Carlos III
2023
Hospital Universitario 12 De Octubre
2021-2023
Centro de Investigación Biomédica en Red
2023
Common variable immunodeficiency (CVID) is characterized by hypogammaglobulinemia and/or a defective antibody response to T-dependent and T-independent antigens. CVID immunization depends on the antigen type, vaccine mechanism, specific patient immune defect. In patients, humoral cellular responses currently used COVID-19 vaccines remain unexplored. Eighteen subjects receiving 2-dose anti-SARS-CoV-2 were prospectively studied. S1-antibodies S1-specific IFN-γ T cell determined ELISA...
The timing of the development specific adaptive immunity after natural SARS-CoV-2 infection, and its relevance in clinical outcome, has not been characterized depth. Description long-term maintenance both cellular humoral responses elicited by real-world anti-SARS-CoV-2 vaccination is still scarce. Here we aimed to understand optimal protective infection vaccination. We performed an early, longitudinal study S1-, M- N-specific IFN-γ IL-2 T cell anti-S total neutralizing antibodies 88 mild,...
An early analysis of circulating monocytes may be critical for predicting COVID-19 course and its sequelae. In 131 untreated, acute patients at emergency room arrival, showed decreased surface molecule expression, including low HLA-DR, in association with an inflammatory cytokine status limited anti-SARS-CoV-2-specific T cell response. Most these alterations had normalized post-COVID-19 6 months after discharge. Acute transcriptome upregulation anti-inflammatory tissue repair genes such as...
SARS-CoV-2 vaccination has proven the most effective measure to control COVID-19 pandemic. Booster doses are being administered with limited knowledge on their need and effect immunity.To determine duration of specific T cells, antibodies neutralization after 2-dose vaccination, assess a third dose adaptive immunity explore correlates protection against breakthrough infection.12-month longitudinal assessment SARS-CoV-2-specific IgG neutralizing triggered by 2 BNT162b2 followed mRNA-1273 in...
Despite the growing number of patients with persistent symptoms after acute SARS-CoV-2 infection, pathophysiology underlying long-COVID is not yet well characterized, and there no established therapy. We performed a deep immune profiling in nine (PSP), before 4-day prednisone course, five post-COVID-19 without (NSP). PSP showed perturbed distribution circulating mononuclear cell populations. Symptoms were accompanied by pro-inflammatory phenotype characterized increased conventional...
Severe acute respiratory syndrome coronavirus 2-specific cell-mediated immunity (SARS-CoV-2-CMI) elicited by mRNA-based vaccines in solid organ transplant (SOT) recipients and its correlation with antibody responses remain poorly characterized.We included 44 (28 kidney, 14 liver, 2 double organ) who received the full series of mRNA-1273 vaccine. SARS-CoV-2-CMI was evaluated at baseline, before second dose, wk after completion vaccination an ELISpot-based interferon-γ FluoroSpot assay using...
The magnitude and kinetics of severe acute respiratory syndrome coronavirus 2-specific cell-mediated immunity (SARS-CoV-2-CMI) in kidney transplant (KT) recipients remain largely unknown.We enumerated SARS-CoV-2-specific interferon-γ-producing CD69+ CD4+ CD8+ T cells at months 4 6 from the diagnosis disease 2019 (COVID-19) 21 KT by intracellular cytokine staining. Overlapping peptides encompassing SARS-CoV-2 spike (S) glycoprotein N-terminal 1- to 643-amino acid sequence membrane protein...
Whether immunosuppression impairs severe acute respiratory syndrome coronavirus 2-specific T cell-mediated immunity (SARS-CoV-2-CMI) after liver transplantation (LT) remains unknown. We included 31 LT recipients in whom SARS-CoV-2-CMI was assessed by intracellular cytokine staining (ICS) and interferon (IFN)-γ FluoroSpot assay a median of 103 days from COVID-19 diagnosis. Serum SARS-CoV-2 IgG antibodies were measured ELISA. A control group nontransplant immunocompetent patients matched (1:1...
The rapid development of vaccines to prevent COVID-19 has raised the need compare capacity different in terms developing a protective humoral response. Previous studies have shown inconsistent results this area, highlighting importance further research evaluate efficacy vaccines. This study utilized highly sensitive and reliable flow cytometry method measure titers IgG1 isotype antibodies blood healthy volunteers after receiving one or two doses various administered Spain. was also used...
Primary infection and/or reactivation of cytomegalovirus (CMV) in kidney transplant recipients (KTR) favor rejection and mortality. T follicular helper cells (TFH) could contribute to protection against CMV. Circulatory TFH (cTFH) were studied pretransplant early posttransplant 90 CMV seropositive KTR not receiving antithymocyte globulin or antiviral prophylaxis, followed-up for 1 year. Patients who presented had significantly lower cTFH activated posttransplant. Pretransplant also within...
Immune dysregulation in individuals with Down syndrome (DS) leads to an increased risk for hospitalization and death due coronavirus disease 2019 (COVID-19) may impair the generation of protective immunity after vaccine administration.
The immunogenicity elicited by the Omicron BA.4/BA.5-adapted bivalent booster vaccine after solid organ transplantation (SOT) has not been characterized.
ABSTRACT The rapid development of vaccines to prevent infection by SARS-CoV-2 virus causing COVID-19 makes necessary compare the capacity different in terms a protective humoral response. Here, we have used highly sensitive and reliable flow cytometry method measure titers antibodies IgG1 isotype blood healthy volunteers after receiving one or two doses being administered Spain. We took advantage multiplexed simultaneously reactivity with S protein original strain Wuhan variants B.1.1.7...
Introduction: The infection and/or reactivation of latent CMV and CMV-disease in kidney transplant patients (KTR) increasing the allo-sensitization risk limiting possibilities getting a new transplant. Cellular response is thought to be responsible CMV-immunity. However, it still unknown if T follicular helper cells (TFH), that are specialized assisting B cell differentiation towards antibody-producing-plasma cells, may contribute protection through promoting neutralizing antibodies (Nabs)....