A5084014072- Clinical Nutrition and Gastroenterology
- Renal Transplantation Outcomes and Treatments
- Organ Transplantation Techniques and Outcomes
- Immune Cell Function and Interaction
- IL-33, ST2, and ILC Pathways
- Eosinophilic Esophagitis
- COVID-19 Clinical Research Studies
- Long-Term Effects of COVID-19
- Transplantation: Methods and Outcomes
- T-cell and B-cell Immunology
- Pediatric Hepatobiliary Diseases and Treatments
- Liver Disease and Transplantation
- Adenosine and Purinergic Signaling
- Microscopic Colitis
- Cytomegalovirus and herpesvirus research
- Organ and Tissue Transplantation Research
- COVID-19 and healthcare impacts
- Immunotherapy and Immune Responses
- Complement system in diseases
- Xenotransplantation and immune response
- Immune Response and Inflammation
- Organ Donation and Transplantation
- Hematopoietic Stem Cell Transplantation
- Mechanical Circulatory Support Devices
- Mesenchymal stem cell research
Research Institute Hospital 12 de Octubre
2014-2025
Hospital Universitario 12 De Octubre
2011-2023
Hospital Universitario La Paz
2021
We examined intraepithelial lymphocytes (IELs) in 213 ileal biopsies from 16 bowel grafts and compared them with 32 native intestines. During the first year posttransplantation, exhibited low levels of IELs (percentage CD103+ cells) principally due to reduced CD3+CD8+ cells, while CD103+CD3– cell numbers became significantly higher. Changes IEL subsets did not correlate histology results, isolated intestine, or multivisceral transplants, but CD3– were higher patients receiving...
Background Prognostic biomarkers of acute rejection (AR) in solid organ transplantation have been addressed multiple small retrospective studies, and there is a critical need for multicenter studies. Because their tolerogenic properties, regulatory T cells (Tregs) play an important role transplant outcome. Methods In the present study, we retrospectively examined different Treg subpopulations independent cohort kidney patients within first year after transplantation. All participating...
The current shortage of pediatric multivisceral donors accounts for the long time and mortality on waiting list patients. use after cardiac death, especially outbreak normothermic regional perfusion, has increased in recent years all solid organs except intestine, mainly because its higher susceptibility to ischemia-reperfusion injury. We present first literature case death transplantation a 13-month-old recipient from 2.5-month-old donor. Once exitus was certified, an extracorporeal...
Rejection is one of the most important drawbacks for graft and patient survival in intestinal multivisceral transplantation. However, there no consensus on diagnostic criteria humoral rejection, literature about role donor-specific antibodies (DSA) allograft outcome risk factors that contribute to their development scant with contradictory results. The present study analyzes DSA exclusively a pediatric cohort 43 transplants. Among our patients, 11.6% showed preformed DSA, but they did not...
Background Intestinal transplantation (ITx) represents the only curative option for patients with irreversible intestinal failure. Nevertheless, its rejection rate surpasses that of other solid organ transplants due to heightened immunological load gut. Regulatory T-cells (Tregs) are key players in induction and maintenance peripheral tolerance, suggesting their potential involvement modulating host vs . graft responses after ITx. Thus, we investigated association Tregs allograft outcomes...
Pretransplantation anti-major histocompatibility complex class I chain-related molecule A (MICA) sensitization is an uncommon event and its role on kidney graft evolution not completely defined.A retrospective study of patients transplanted between 2005 2011 in our center (n=727) was performed. Recipients were classified four groups, according either to multiplexed flow cytometry-recorded anti-human leukocyte antigen (HLA) anti-MICA antibodies or percent panel-reactive antibody (PRA; by...
Whether immunosuppression impairs severe acute respiratory syndrome coronavirus 2-specific T cell-mediated immunity (SARS-CoV-2-CMI) after liver transplantation (LT) remains unknown. We included 31 LT recipients in whom SARS-CoV-2-CMI was assessed by intracellular cytokine staining (ICS) and interferon (IFN)-γ FluoroSpot assay a median of 103 days from COVID-19 diagnosis. Serum SARS-CoV-2 IgG antibodies were measured ELISA. A control group nontransplant immunocompetent patients matched (1:1...
We previously reported that preformed anti-MHC class I–related chain A (MICA) antibodies increase the risk for renal graft rejection and enhance deleterious effect of PRA+ status early after transplantation. studied 727 kidney recipients. Days to reach optimal serum creatinine level, estimated glomerular filtration rate (eGFR) at Month 3 chronic disease (CKD) stages were recorded. Anti-MICA specificities C1q binding tested by solid-phase assay. Complement-dependent cytotoxicity (CDC) flow...
Background Human CD8 immunodeficiency is characterized by undetectable CD8+ lymphocytes and an increased population of CD4−CD8− (double negative) T lymphocytes.Design Methods We hypothesized that the double negative subset corresponds to cellular should express committed cytotoxic lymphocyte lineage. To assess this, we determined phenotype function peripheral blood mononuclear cells and/or magnetically isolated from two CD8-deficient patients. analyze expression co-localization with...
There is an urgent need to address the shortage of potential multivisceral grafts in order reduce average time waiting list. Since donation after circulatory death (DCD) has been successfully employed for other solid organs, a thorough evaluation use intestinal from DCD warranted. Here, we have generated model Maastricht III rodents, focusing on viability and at five (DCD5) twenty (DCD20) minutes cardiac arrest compared living brain donors. groups exhibited time-dependent damage. DCD20...
Background Donor-specific anti-HLA antibodies (DSA) impact negatively on the outcome of intestinal grafts. Although use antibody-removal therapies (ART) is becoming more frequent in last few years, issues regarding their timing and effectiveness remain under discussion. Methods In present study, we report our experience with eight ART procedures (based plasmapheresis, intravenous immunoglobulin, rituximab) pediatric multivisceral transplants de novo DSA (dnDSA). Results were performed when...
“Slow” and “Fast” C3 complement variants (C3S C3F) result from a g.304C>G polymorphism that changes arginine to glycine at position 102. are associated with complement-mediated diseases outcome in transplantation. In this work genotyping is achieved by Real Time PCR - High Resolution Melting (RT-PCR-HRM) optimized method. an analysis of 49 subjects, 10.2% were C3FF, 36.7% C3SF 53.1% C3SS. Allelic frequencies (70% for C3S 30% Hardy-Weinberg equilibrium similar those published previously....
Chronic Granulomatous Disease (CGD) is a rare primary immunodeficiency caused by the alteration of enzyme complex NADPH oxidase, which affects phagocytic function. CGD patients are susceptible to recurrent infections mainly bacteria and/or fungi.We studied 6 year-old boy with suspicion CGD. The diagnosis was confirmed based on functional study oxidase. Simultaneously, second pregnancy mother reported and genetic counselling requested.We identified new disease-causing mutation direct...
Mesenchymal stromal cells (MSC) have been proposed as a promising complement to standard immunosuppression in solid organ transplantation because of their immunomodulatory properties. The present work addresses the role adipose-derived MSC (Ad-MSC) an experimental model acute rejection small bowel (SBT).Heterotopic allogeneic SBT was performed. A single dose 1.5x106 Ad-MSC intra-arterially delivered just before graft reperfusion. Animals were divided into CONTROL (CTRL), CONTROL+Ad-MSC...
Considering recent clinical and experimental evidence, expectations for using DCD-derived intestines have increased considerably. However, more knowledge about DCD procedure long-term results after intestinal transplantation (ITx) is needed. We aimed to describe in detail a ITx normothermic regional perfusion (NRP) preclinical model. Small bowel was obtained from pigs donors 1 h of NRP transplanted the recipients. Graft Intestinal samples were during transplantation. Ischemia-reperfusion...
Introduction: Lack of enough space for safe closure after transplantation leads to the use fascia and abdominal wall grafts overcome this problem. Little is known about immunogenicity fascial transplants. We have been using an experimental model transplants in rabbits last 4 years. Here we show results a subset 9 pig rabbit xenotransplants. Methods: Fascia procurement: midline incision served 3 x1 cm graft procurement. The was kept at 4°C serum (7) or glutaraldehyde (G) (2) transplanted 24 h...