Long non-coding RNAs (lncRNAs) are involved in regulating chromatin modifications, gene transcription, mRNA translation, and protein function. We recently reported a high variation the basal expression levels of panel lncRNAs HeLa MCF-7 cells their differential response to DNA damage induction. Here, we hypothesized that lncRNA molecules with different cellular may have abundance secreted exosomes, exosome would reflect damage. MALAT1, HOTAIR, lincRNA-p21, GAS5, TUG1, CCND1-ncRNA exosomes...

Exosomes are membranous vesicles containing various biomolecules including lncRNAs which involved in cellular communication and secreted from many cells cancer cells. In our study, investigated the exosomal GAS5 lincRNA-p21 lncRNA levels urine samples 30 patients with prostate (PCa) 49 benign prostatic hyperplasia. Quantification of molecules was performed by real-time PCR. We observed a significant difference between PCa BPH whereas did not reveal difference. Our data suggest that...

Long non-coding RNAs (lncRNAs) have been shown to be aberrantly expressed in head and neck cancer (HNC). The aim of the present study was evaluate plasma levels three lncRNA molecules (lincRNA-p21, GAS5, HOTAIR) treatment response HNC patients treated with radical chemoradiotherapy (CRT). Forty-one were enrolled study. Most had nasopharyngeal carcinoma (n = 27, 65.9 %) locally advanced disease. Blood drawn at baseline evaluation 4.5 months after therapy. lncRNAs measured by semiquantitative...

Long non-coding RNAs (lncRNAs) are key regulatory molecules in many fundamental cellular processes and their deregulation is assumed to contribute carcinogenesis. Exosomal lncRNAs thought be involved the dissemination of cell signals control local microenvironments. In current study, exosomal expression growth arrest specific 5 (GAS5), an inhibitor proliferation promoter apoptosis, was evaluated apoptotic initiated by different mechanisms. Therefore, MCF-7 MDA-MB-231 breast cancer cells were...

BACKGROUND: Novel biomarkers are needed to predict the effectiveness of treatment presurgical neoadjuvant chemotherapy (NAC) in breast cancer (BC). OBJECTIVE: This is an exploratory study assess impact 3 cancer-related long non-coding RNAs (lncRNAs) (H19, MALAT1 and GA5) blood p lasma patients with BC predicting response NAC. METHODS: The plasma levels were relatively measured by quantitative PCR at baseline, end fourth cycle NAC locally advanced BC. RESULTS: Only H19 was associated...

Abstract Objectives Multiple myeloma (MM) is a common hematological cancer. Hence, it important to conduct further studies investigating the molecular mechanisms in detail that contributes genesis. In addition genetic changes, epigenetic factors such as miRNAs may influence expression of myeloma-related genes. Methods Our study aimed detect genes closely related MM and involved cancer process by changing these with bioinformatics tools vitro methods. Bioinformatics approaches identified hub...

Circulating DNA is present in plasma/serum, mainly complexed with histones as nucleosomes. The detection of circulating nucleosomes (cNUCs) the peripheral blood may be a diagnostic modality for cancer-associated changes modified histone tails circulation. In study, we investigated correlation between trimethylation H3 lysine 9 (H3K9me3) and H4 20 (H4K20me3), which are hallmarks pericentric heterochromatin, cNUCs healthy subjects patients colorectal cancer (CRC) multiple myeloma (MM). plasma...

microRNAs (miRNAs) are small molecules in their mature form and master regulators of gene expression. Recent work has shown that miRNAs involved the p53 network. Of various miRNAs, miR-34 is regulated by protein. can be induced ionizing radiation (IR) vitro vivo. However, there no data literature for induction a chemical agent inducing DNA damage. Here we studied expression HeLa MCF-7 cells exposed to genotoxic stress-induced bleomycin (BLM) or γ-radiation. We first analyzed accumulation...

Background/Aim: Prostate cancer (PCa) is an androgen-dependent disease. Novel anti-androgens (i.e. enzalutamide) have recently been developed for the treatment of patients with metastatic castration-resistant prostate (CRPC). Evidence accumulating that antigen 3 (PCA3) involved in androgen receptor (AR) signaling. Here, combination enzalutamide-mediated AR blockade, we investigated effect PCA3 targeting on viability PCa cells. Materials and Methods: In hormone-sensitive LNCaP cells,...

Background/Aim: Colorectal cancer (CRC) is one of leading cancers in terms incidence and mortality. Interaction tumor cells with the surrounding microenvironment plays a crucial role development progression CRC. Many pathways such as kynurenine pathway, OX40/OX40L-mediated signaling microRNAs targeting PD-L1 may be involved CRC by affecting T cell activation, thus creating an immune-deficient microenvironment. Herein, our goal was to assess association between plasma levels tryptophan (TRP),...

Background/Aim: No blood-based biomarkers are available to differentiate between colonic tumors and precancerous polyps. Previously we demonstrated levels of trimethylated H4K20 (H4K20me3) be lower in blood plasma from patients with colon cancer than those cancer-free individuals. Herein, added individuals polyps for the first time order analyze investigate usefulness H4K20me3 histone H4 discriminate Materials Methods: The study included a cohort 185 undergoing colonoscopy. H4, measured by...

In the present study, cellular or exosomal expression of H19, an oncofetal lncRNA gene, was evaluated during androgen stimulation via dihydrotestosterone (DHT) AR blockage enzalutamide in cultured hormone-sensitive Pca cells which overexpres (LNCaP-AR+).Prostate cancer (PCa) is androgen-dependent disease. Androgen receptor (AR) antagonists (i.e. enzalutamide) have been used for treatment patients with metastatic castration-resistant prostate (CRPC). Exosomes and their contents (non-coding...

The effect of radiation-induced genotoxic stress on the expression satellite II and III repeats in breast colon cancer cells

The principal aim of our study was to investigate the usefulness serum protein and circulating mRNA insulin-like growth factor-1 (IGF-1) as a diagnostic prognostic tool in hepatocellular carcinoma (HCC). Fifty-four HCC patients age- sex-matched 20 healthy controls were enrolled into this study. Pretreatment IGF-1 determined by solid-phase sandwich ELISA quantitative RT-PCR method, respectively. median age at diagnosis 60 years, range 36–77 years; where majority group male (n = 48, 88.8 %)....

BACKGROUND: Reseptor tyrosine kinases (cMET and EGFR) are important in lung cancer targeted therapy. We believe if we can use them as markers for clinicians to help decide the diagnosis of cancer. This parameter will be serum samples patients with trea tment. The aim this study is aimed evaluate clinical utility protein circulating mRNA cMET HGF patients. also analyzed correlation expression clinicopathologic parameters. METHODS: performed enzyme-linked immunosorbent assay (ELISA) measure...

Breast cancer is the most common type of in women worldwide. Triple methylation H4 lysine 20 (H4K20me3), a key component epigenetic regulation genomic integrity, catalyzed by methyltransferase, SUV420H2. Data on expression status SUV420H2 breast are limited. In present study, influence suppression proliferation cells was experimentally investigated. Subsequently, assessed resectable along with H4K20me3 status. knocked down using small interfering RNA oligonucleotides. determined...

Histone modifications are involved in the DNA damage response (DDR). Here, by utilizing an ELISA immunoassay we assessed methylation at H3K9 (H3K9me2 and H3K9me3) two cell lines with differential sensitivity to radiation-induced apoptosis, HeLa (sensitive) MCF-7 (resistant). We found that induction γ-irradiation leads considerable accumulation (up 5-fold) of H3K9me2 H3K9me3, but not H4K20me3 (control modification) cells (p<0.05). Interestingly, a lower dose (2 Gy) was more effective than 5...