A5001084037- Prenatal Screening and Diagnostics
- Genomic variations and chromosomal abnormalities
- Chromosomal and Genetic Variations
- Neurobiology and Insect Physiology Research
- Photoreceptor and optogenetics research
- Receptor Mechanisms and Signaling
- Angiogenesis and VEGF in Cancer
- Reproductive Biology and Fertility
- RNA Interference and Gene Delivery
- Salivary Gland Tumors Diagnosis and Treatment
- Cell death mechanisms and regulation
- Genetic Syndromes and Imprinting
- Lymphatic System and Diseases
- Medical Imaging and Pathology Studies
- Occupational and environmental lung diseases
- Signaling Pathways in Disease
- Physiological and biochemical adaptations
- Peroxisome Proliferator-Activated Receptors
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- HER2/EGFR in Cancer Research
- Assisted Reproductive Technology and Twin Pregnancy
- Science, Research, and Medicine
- Pituitary Gland Disorders and Treatments
- Sexual Differentiation and Disorders
- Neurological diseases and metabolism
Duke-NUS Medical School
2024
Peking University
2019-2023
Chinese Institute for Brain Research
2019-2023
Xianyang Research and Design Institute of Ceramics (China)
2023
McGovern Institute for Brain Research
2022
King University
2022
Universiti Sains Malaysia
2019-2020
Central South University
2008-2015
National Engineering Research Center of Human Stem Cells
2013-2015
Juntendo University
2010
Preimplantation genetic diagnosis and screening are widely accepted for chromosomal abnormality identification to avoid transferring embryos with defects. Massively parallel sequencing (MPS) is a rapidly developing approach genome analysis increasing application in clinical practice. The purpose of this study was use MPS aneuploidies unbalanced rearrangements after blastocyst biopsy. Trophectoderm (TE) samples 38 blastocysts from 16 vitro fertilization cycles were subjected analysis....
Next generation sequencing (NGS) is now being used for detecting chromosomal abnormalities in blastocyst trophectoderm (TE) cells from vitro fertilized embryos. However, few data are available regarding the clinical outcome, which provides vital reference further application of methodology. Here, we present a evaluation NGS-based preimplantation genetic diagnosis/screening (PGD/PGS) compared with single nucleotide polymorphism (SNP) array-based PGD/PGS as control. A total 395 couples...
STUDY QUESTIONIs preimplantation genetic diagnosis (PGD) for translocation carriers more effective when done with a single-nucleotide polymorphism (SNP) array using trophectoderm (TE) biopsy and frozen embryo transfer (FET) compared traditional PGD based on fluorescence in situ hybridization (FISH-PGD) blastomere fresh transfer?
Copy number variations (CNVs), a common genomic mutation associated with various diseases, are important in research and clinical applications. Whole genome amplification (WGA) massively parallel sequencing have been applied to single cell CNVs analysis, which provides new insight for the fields of biology medicine. However, WGA-induced bias significantly limits sensitivity specificity detection. Addressing these limitations, we developed practical bioinformatic methodology detection at...
Development of resistance to chemo- and radiotherapy in patients suffering from advanced cervical cancer narrows the therapeutic window for conventional therapies. Previously we reported that a combination selective BCL-2 family inhibitors ABT-263 A-1210477 decreased cell proliferation C33A, SiHa CaSki human lines. As binds both BCL-XL with high affinity, it was unclear whether synergism drug driven either by singly inhibiting or BCL-XL, inhibition both. In this present study, used inhibitor...
The monoamine neuromodulator dopamine (DA) plays a critical role in the brain, and ability to directly measure dopaminergic activity is essential for understanding its physiological functions. We therefore developed first red fluorescent GPCR-activation–based DA (GRAB ) sensors optimized versions of green GRAB following our previous studies. In response extracellular DA, both have large increase fluorescence (ΔF/F 0 values 150% 340%, respectively), with subcellular resolution, subsecond...
The ability to directly measure acetylcholine (ACh) release is an essential first step towards understanding its physiological function. Here we optimized the GRAB ACh ( G PC R - A ctivation– B ased- ) sensor with significantly improved sensitivity and minimal downstream coupling. Using this sensor, measured in-vivo cholinergic activity in both Drosophila mice, revealing compartmental signals fly olfactory center single-trial dynamics multiple regions of mice brain under a variety different behaviors
Translocation is one of the more common structural rearrangements chromosomes, with a prevalence 0.2%. The two most types chromosomal translocations, Robertsonian and reciprocal, usually result in no obvious phenotypic abnormalities when balanced. However, these are still associated reproductive risks, such as infertility, spontaneous abortion delivery babies mental retardation or developmental delay. In recent years, array-based whole-genome amplification (WGA) technologies, including...
Copy Number Variants (CNVs) is a new molecular frontier in clinical genetics. CNVs 1p36 are usually pathogenic and have attracted the attention of cytogeneticists worldwide. None triplication has been reported thus far.We present three patients with 1p36. Among them one first tetrasomy due to pure microtriplication other two microdeletion. Traditional chromosome G-banding technique showed normal karyotype. Single nucleotide polymorphism (SNP) microarray analysis combined multiplex...
Abstract The microstructure design is important for regulating the microwave dielectric properties of materials. However, in‐depth studies on frequency temperature stability and related micromechanism remain poorly understood. work investigates correlation among sintering behavior, crystal structure, bonding nature, LnPO 4 (Ln = Eu, Pr) ceramics by combining first‐principles calculations experimental perspective. high density ( ρ > 97%) large grains associated with lattice expansion...
Abstract Temporal coincidence between the conditioned stimulus (CS) and unconditioned (US) is essential for associative learning across species. Despite its ubiquitous presence, mechanism that may regulate this time window duration remains unclear yet. Using olfactory in Drosophila as a model, we find suppressing or promoting serotonin (5-HT) signal could respectively shorten prolong of odor-shock synaptic plasticity mushroom body (MB) Kenyon cells (KCs). Capitalizing on G PC R - ctivation b...
Researchers have recently demonstrated that thrombospondin-1 (TSP-1) has an important function in regulating neovascularization. Whether it inhibits or accelerates neovascularization, however, is still controversial. We found few reports about the correlation between TSP-1 and vascularization mucoepidermoid carcinoma. In this research, distribution expression of carcinoma were investigated. also analyzed (1) microvessel density (MVD), as indicator neovascularization activity, (2) effect on...
Objective To determine the karyotype of a patient with Prader-Willi-like syndrome features. Methods Chromosomal high resolution banding was carried out to analyze patient, and methylation-specific PCR used imprinting region chromosome 15.Subtelomeric screened by multiplex ligation-dependent probe amplification （MLPA）, fluorescent in situ hybridization （FISH） real-time quantitative were further performed identify deleted region. Results No abnormality discovered analysis studies. MLPA showed...
Objective To establish a single-cell whole genome amplification (WGA) technique, in combination with comparative genomic hybridization (CGH), for analyzing chromosomal copy number changes, and to explore its clinical application preimplantation genetic diagnosis (PGD). Methods Twelve single cell samples known karyotypes, including 5 chorionic villus samples, 4 human embryonic stem (hESC) 3 peripheral lymphocyte blastomere carrying abnormalities detected by PGD, were collected combining...
Summary Impaired proteostasis can induce protein aggregation which is toxic to neuronal cells, contributing neurodegeneration and other signs of aging. In this study, we delineate an early-onset progressive neuropathy evoking Giant Axonal Neuropathy 1. The causative gene IVNS1ABP encodes a E3-ubiquitin ligase adaptor close gigaxonin paralogue. Patient-derived fibroblasts, iPSCs, neural progenitors exhibited hallmarks accumulation lysosomal dysfunction. Ubiquitome analysis revealed...