A5001158217- Pregnancy and preeclampsia studies
- Hepatitis C virus research
- interferon and immune responses
- Systemic Lupus Erythematosus Research
- Birth, Development, and Health
- Prenatal Screening and Diagnostics
- Gestational Diabetes Research and Management
- Assisted Reproductive Technology and Twin Pregnancy
- Fetal and Pediatric Neurological Disorders
- Influenza Virus Research Studies
- Inflammasome and immune disorders
- Maternal and fetal healthcare
- MRI in cancer diagnosis
- Protease and Inhibitor Mechanisms
- Adrenal Hormones and Disorders
- Immune Response and Inflammation
- Renal and Vascular Pathologies
- Renal Diseases and Glomerulopathies
- Peroxisome Proliferator-Activated Receptors
- Adrenal and Paraganglionic Tumors
- RNA modifications and cancer
- Brucella: diagnosis, epidemiology, treatment
- Hepatitis Viruses Studies and Epidemiology
- Blood Coagulation and Thrombosis Mechanisms
- Silymarin and Mushroom Poisoning
The University of Texas Southwestern Medical Center
2007-2023
Southwestern Medical Center
2008-2023
Parkland Health & Hospital System
2023
Cornell University
2010
Foundation for Reproductive Medicine
2010
University of Washington
2008-2009
Czech Academy of Sciences, Institute of Microbiology
2007
Beijing Obstetrics and Gynecology Hospital
2006
St. Mary's Hospital
1993-2000
St Mary's Hospital
1995
RIG-I is an RNA helicase containing caspase activation and recruitment domains (CARDs). binding signaling by are implicated in pathogen recognition triggering of IFN-alpha/beta immune defenses that impact cell permissiveness for hepatitis C virus (HCV). Here we evaluated the processes control signaling. studies analysis cells lacking RIG-I, or related MDA5 protein, demonstrated but not MDA5, efficiently binds to secondary structured HCV confer induction IFN-beta expression. We also found...
Hepatitis C virus (HCV) and triglyceride-rich very low-density lipoproteins (VLDLs) both are secreted uniquely by hepatocytes circulate in blood a complex. Here, we isolated from human hepatoma cells the membrane vesicles which HCV replicates. These vesicles, contain replication complex, highly enriched proteins required for VLDL assembly, including apolipoprotein B (apoB), apoE, microsomal triglyceride transfer protein. In that constitutively produce infectious HCV, production is reduced...
Viral signaling through retinoic acid-inducible gene-I (RIG-I) and its adaptor protein, IFN promoter-stimulator 1 (IPS-1), activates regulatory factor-3 (IRF-3) the host IFN-alpha/beta response that limits virus infection. The hepatitis C (HCV) NS3/4A protease cleaves IPS-1 to block RIG-I signaling, but how this regulation controls HCV is not known. Moreover, endogenous cleavage has been demonstrated in context of infection vitro or vivo. Here, we show transiently induces RIG-I-...
Silymarin, an extract from milk thistle (Silybum marianum), and its purified flavonolignans have been recently shown to inhibit hepatitis C virus (HCV) infection, both in vitro vivo. In the current study, we further characterized silymarin's antiviral actions. Silymarin had effects against cell culture (HCVcc) infection that included inhibition of entry, RNA protein expression, infectious production. did not block HCVcc binding cells but inhibited entry several viral pseudoparticles (pp),...
Interferon regulatory factor-3 (IRF-3) activation directs α/β interferon production and interferon-stimulated gene (ISG) expression, which limits virus infection. Here, we examined the distribution of hepatitis C (HCV) nonstructural 3 protein, status IRF-3 activation, expression target genes ISGs during asynchronous HCV infection in vitro liver biopsies from patients with chronic infection, using confocal microscopy functional genomics approaches. In general, stimulated a low-frequency...
We investigated the mechanisms of alphafetoprotein (AFP) transfer across human placenta by correlating measurements AFP with cytochemical localization AFP. Placental cotyledons were dually perfused in vitro either fetal or maternal perfusate containing umbilical cord plasma as a source Steady state clearance, corrected for release endogenous AFP, was 0.973 +/- 0.292 microliter/min per gram to direction (n = 10), significantly higher (P < 0.02) than that 5; 0.022 0.013 gram). Clearance...
Chronic hepatitis C virus (HCV) infection is a major global public health problem. HCV supported by viral strategies to evade the innate antiviral response wherein NS3.4A protease complex targets and cleaves interferon promoter stimulator-1 (IPS-1) adaptor protein ablate signaling of alpha/beta immune defenses. Here we examined structural requirements therapeutic potential inhibitors control against infection. The composition NS3 includes an amino-terminal serine domain carboxyl-terminal RNA...
We tested two hypotheses: 1) that fibrin-containing fibrinoid-filled denudations of the syncytiotrophoblast may provide a route for paracellular diffusion and 2) placentas from women who had elevated maternal serum alphafetoprotein (MSAFP) in midgestation raised permeability to AFP greater denudation than normal pregnancy. measured creatinine clearance across term placental cotyledons above groups used light microscope morphometric analysis determine volume density fibrinoid deposits. There...
OBJECTIVE In addition to the physical symptoms of galactorrhoea and amenorrhoea, hyperprolactinaemia in women is also reported be associated with psychological symptoms. Previous studies have found an increased incidence depression, anxiety hostility female patients hyperprolactinaemia. this study, were assessed a large population symptom scores compared between definite evidence pituitary adenoma on high‐resolution CT scanning those without, who presumed idiopathic or ‘functional’ DESIGN...
hIMEMORANDA.tM, 1211a contiinuous suture of catgut.The wound in the abdomninal wall was then closed by continuous catgut sutures, peritoneum, muscles, and skin being eachl stitchled separately.The operation very well borne.As patient re- covered consciousness slhe became wild restless.Shle soon began to complain pain abdomen, it evident that labour lhad commenced.A healthy- looking male infant born about 3 A.M., twelve lhours after commencement operation.The pulsation cord hiad ceased, no...
The placenta is a dynamic organ that must perform remarkable variety of functions during its relatively short existence in order to support developing fetus. These include nutrient delivery, gas exchange, waste removal, hormone production, and immune barrier protection. Proper development function are critical for healthy pregnancy outcomes, but the underlying genomic regulatory events control this process remain largely unknown. We hypothesized mapping sites transcriptional enhancer...
Magnetic resonance imaging (MRI) has gained popularity in the field of prenatal due to ability provide high quality images soft tissue. In this paper, we presented a novel method for extracting different textural and morphological features placenta from MRI volumes using topographical mapping. We proposed polar planar mapping methods produce common placental unique point observation. The extracted included entire surface, as well thickness, intensity, entropy maps displayed convenient...
SummaryMaternal serum α-fetoprotein is more commonly elevated (> 2 multiples of the median) in pregnancy associated with systemic lupus erythematosus (35 per cent compared 5 for a general obstetric population, P < 001). An level is, this series, predictor fetal loss, induced hypertension and premature delivery (before 34 weeks) patient.
Magnetic resonance imaging (MRI) has potential benefits in understanding fetal and placental complications pregnancy. An accurate segmentation of the uterine cavity placenta can help facilitate fast automated analyses accreta spectrum other pregnancy complications. In this study, we trained a deep neural network for fully automatic from MR images pregnant women with without abnormalities. The two datasets were axial MRI data 241 women, among whom, 101 patients also had sagittal data. Our...