Andrew Champion
A5090265250- Neurobiology and Insect Physiology Research
- Insect and Arachnid Ecology and Behavior
- Neural dynamics and brain function
- Genetics, Aging, and Longevity in Model Organisms
- Plant Molecular Biology Research
- Zebrafish Biomedical Research Applications
- Cell Image Analysis Techniques
- Animal Behavior and Reproduction
- Advanced Electron Microscopy Techniques and Applications
- Advanced Fluorescence Microscopy Techniques
- Physiological and biochemical adaptations
- Plant and Biological Electrophysiology Studies
- Circadian rhythm and melatonin
- Visual Attention and Saliency Detection
- Axon Guidance and Neuronal Signaling
- Scientific Computing and Data Management
- Colorectal Cancer Screening and Detection
- Retinal Development and Disorders
- Single-cell and spatial transcriptomics
- Planarian Biology and Electrostimulation
- AI in cancer detection
- Radiomics and Machine Learning in Medical Imaging
- Photoreceptor and optogenetics research
- Neuroinflammation and Neurodegeneration Mechanisms
- Visual perception and processing mechanisms
University of Cambridge
2022-2025
MRC Laboratory of Molecular Biology
2023-2024
Janelia Research Campus
2015-2023
Howard Hughes Medical Institute
2016-2023
Georgia Institute of Technology
2014
Neuronal circuit mapping using electron microscopy demands laborious proofreading or reconciliation of multiple independent reconstructions. Here, we describe new methods to apply quantitative arbor and network context iteratively proofread reconstruct circuits create anatomically enriched wiring diagrams. We measured the morphological underpinnings connectivity in existing reconstructions Drosophila sensorimotor (larva) visual (adult) systems. Synaptic inputs were preferentially located on...
High-resolution electron microscopy of nervous systems has enabled the reconstruction synaptic connectomes. However, we do not know sign for each connection (i.e., whether a is excitatory or inhibitory), which implied by released transmitter. We demonstrate that artificial neural networks can predict transmitter types presynapses from micrographs: network trained to six transmitters (acetylcholine, glutamate, GABA, serotonin, dopamine, octopamine) achieves an accuracy 87% individual...
Abstract Animal behavior is principally expressed through neural control of muscles. Therefore understanding how the brain controls requires mapping neuronal circuits all way to motor neurons. We have previously established technology collect large-volume electron microscopy data sets tissue and fully reconstruct morphology neurons their chemical synaptic connections throughout volume. Using these tools we generated a dense wiring diagram, or connectome, for large portion Drosophila central...
Animal behavior is principally expressed through neural control of muscles. Therefore understanding how the brain controls requires mapping neuronal circuits all way to motor neurons. We have previously established technology collect large-volume electron microscopy data sets tissue and fully reconstruct morphology neurons their chemical synaptic connections throughout volume. Using these tools we generated a dense wiring diagram, or connectome, for large portion Drosophila central brain....
In most animals, a relatively small number of descending neurons (DNs) connect higher brain centers in the animal’s head to circuits and motor (MNs) nerve cord body that effect movement limbs. To understand how signals generate behavior, it is critical these pathways are organized onto MNs. fly, Drosophila melanogaster , MNs controlling muscles leg, wing, other systems reside ventral (VNC), analogous mammalian spinal cord. companion papers, we introduced densely-reconstructed connectome Male...
Nervous systems function as ensembles of neurons communicating via synaptic connections, and a functional understanding nervous requires extensive knowledge their connectomes. In companion paper (Takemura et al., 2023), we describe the acquisition complete fruit fly nerve cord connectome, first for an animal that can walk or fly. Here, to efficiently navigate appreciate biological significance this categorise name nearly all systematically link them experimental literature. We employ system...
Abstract High-resolution electron microscopy of nervous systems enables the reconstruction connectomes. A key piece missing information from connectomes is synaptic sign. We show that for D. melanogaster , artificial neural networks can predict transmitter type released at synapses micrographs and thus add putative signs to connections. Our network discriminates between six transmitters (acetylcholine, glutamate, GABA, serotonin, dopamine, octopamine) with an average accuracy 87%/94%...
Abstract In most animals, a relatively small number of descending neurons (DNs) connect higher brain centers in the animal’s head to circuits and motor (MNs) nerve cord body that effect movement limbs. To understand how signals generate behavior, it is critical these pathways are organized onto MNs. fly, Drosophila melanogaster , MNs controlling muscles leg, wing, other systems reside ventral (VNC), analogous mammalian spinal cord. companion papers, we introduced densely-reconstructed...
Summary Nervous systems function as ensembles of neurons communicating via synaptic connections, and a functional understanding nervous requires extensive knowledge their connectomes. In companion paper (Takemura et al., 2023), we describe the acquisition complete fruit fly nerve cord connectome, first for an animal that can walk or fly. Here, to efficiently navigate appreciate biological significance this categorise name nearly all systematically link them experimental literature. We employ...
In most complex nervous systems there is a clear anatomical separation between the nerve cord, which contains of final motor outputs necessary for behaviour, and brain. insects, neck connective both physical information bottleneck connecting brain ventral cord (VNC, spinal analogue) comprises diverse populations descending (DN), ascending (AN) sensory neurons, are crucial sensorimotor signalling control. Integrating three separate EM datasets, we now provide complete connectomic description...
In most animals, a relatively small number of descending neurons (DNs) connect higher brain centers in the animal’s head to circuits and motor (MNs) nerve cord body that effect movement limbs. To understand how signals generate behavior, it is critical these pathways are organized onto MNs. fly, Drosophila melanogaster , MNs controlling muscles leg, wing, other systems reside ventral (VNC), analogous mammalian spinal cord. companion papers, we introduced densely-reconstructed connectome Male...
Abstract Neuronal circuit mapping using electron microscopy demands laborious proofreading or reconciliation of multiple independent reconstructions. Here, we describe new methods to apply quantitative arbor and network context iteratively proofread reconstruct circuits create anatomically-enriched wiring diagrams. We measured the morphological underpinnings connectivity in existing reconstructions Drosophila sensorimotor (larva) visual (adult) systems. Synaptic inputs were preferentially...
In most complex nervous systems there is a clear anatomical separation between the nerve cord, which contains of final motor outputs necessary for behaviour, and brain. insects, neck connective both physical an information bottleneck connecting brain ventral cord (an analogue spinal cord) comprises diverse populations descending neurons (DNs), ascending (ANs) sensory neurons, are crucial sensorimotor signalling control. Here, by integrating three separate electron microscopy (EM)...
Animal behavior is principally expressed through neural control of muscles. Therefore understanding how the brain controls requires mapping neuronal circuits all way to motor neurons. We have previously established technology collect large-volume electron microscopy data sets tissue and fully reconstruct morphology neurons their chemical synaptic connections throughout volume. Using these tools we generated a dense wiring diagram, or connectome, for large portion Drosophila central brain....
Nervous systems function as ensembles of neurons communicating via synaptic connections, and a functional understanding nervous requires extensive knowledge their connectomes. In companion paper (Takemura et al., 2023), we describe the acquisition complete fruit fly nerve cord connectome, first for an animal that can walk or fly. Here, to efficiently navigate appreciate biological significance this categorise name nearly all systematically link them experimental literature. We employ system...
Pattern recognition in tissue biopsy images can assist clinical diagnosis and identify relevant image characteristics linked with various biological characteristics. Although previous work suggests several informative imaging features for pattern recognition, there exists a semantic gap between of these pathologists' interpretation histopathological images. To address this challenge, we develop decision support system automated Fuhrman grading renal carcinoma We extract 1316 color, shape,...
Investigating the dense meshwork of wires and synapses that form neuronal circuits is possible with high resolution serial-section electron microscopy (ssEM) 1 . However, imaging scale required to comprehensively reconstruct axons dendrites more than 10 orders magnitude smaller spatial extents occupied by networks interconnected neurons 2 —some which span nearly entire brain. The difficulties in generating handling data for relatively large volumes at nanoscale has thus restricted all...
Larvae of the fruit fly