A5042109411- Mitochondrial Function and Pathology
- Pluripotent Stem Cells Research
- Viral Infections and Immunology Research
- CRISPR and Genetic Engineering
- Cancer Genomics and Diagnostics
- Autophagy in Disease and Therapy
- ATP Synthase and ATPases Research
- RNA Research and Splicing
- Epigenetics and DNA Methylation
- Genomics and Chromatin Dynamics
- Cardiac Fibrosis and Remodeling
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Endoplasmic Reticulum Stress and Disease
- Thyroid Cancer Diagnosis and Treatment
- Systemic Lupus Erythematosus Research
- Renal and related cancers
- Developmental Biology and Gene Regulation
- Metabolism and Genetic Disorders
- Evolution and Genetic Dynamics
- Heat Transfer and Boiling Studies
- Microbial Metabolic Engineering and Bioproduction
- Algal biology and biofuel production
- Cytokine Signaling Pathways and Interactions
- Single-cell and spatial transcriptomics
Guangzhou Institutes of Biomedicine and Health
2016-2025
Guangzhou Medical University
2017-2025
University of Hong Kong
2018-2025
Tongji Hospital
2024
Union Hospital
2013-2024
Huazhong University of Science and Technology
2013-2024
Chinese University of Hong Kong
2019-2024
Shandong Jiaotong University
2024
Chinese Academy of Sciences
2013-2023
State Key Laboratory of Respiratory Disease
2012-2023
Abstract Pluripotent stem cells hold great promise in regenerative medicine and developmental biology studies. Mitochondrial metabolites, including tricarboxylic acid (TCA) cycle intermediates, have been reported to play critical roles pluripotency. Here we show that TCA enzymes Pdha1, Pcb, Aco2, Cs, Idh3a, Ogdh, Sdha Mdh2 are translocated the nucleus during somatic cell reprogramming, primed-to-naive transition totipotency acquisition. The nuclear-localized Idh3a promote reprogramming...
Valproic acid (VPA) is widely used to treat epilepsy, migraine, chronic headache, bipolar disorder, and as adjuvant chemotherapy, but potentially causes idiosyncratic liver injury. Alpers‐Huttenlocher syndrome (AHS), a neurometabolic disorder caused by mutations in mitochondrial DNA polymerase gamma (POLG), associated with an increased risk of developing fatal VPA hepatotoxicity. However, the mechanistic link this clinical mystery remains unknown. Here, fibroblasts from 2 AHS patients were...
Mitochondrial quality control plays an important role in maintaining mitochondrial homeostasis and function. Disruption of degrades brain We found that flunarizine (FNZ), a drug whose chronic use causes parkinsonism, led to parkinsonism-like motor dysfunction mice. FNZ induced decreased mass specifically the brain. content both neurons astrocytes, without affecting number nigral dopaminergic neurons. In human neural progenitor cells, also depletion. Mechanistically, independent ATG5- or...
Induced pluripotent stem cells (iPSCs) have fewer and immature mitochondria than somatic mainly rely on glycolysis for energy source. During cell reprogramming, other organelles get remodeled. However, events of organelle remodeling interaction during reprogramming not been extensively explored. We show that both SKP/SKO (Sox2, Klf4, Pou5f1/Oct4) SKPM/SKOM (SKP/SKO plus Myc/c-Myc) lead to decreased mitochondrial mass but with different kinetics by divergent pathways. Rapid, MYC/c-MYC-induced...
Myocarditis is an inflammatory cardiovascular disease which contributes to dilated cardiomyopathy (DCM) and heart failure. Canagliflozin (CANA) exerts anti-inflammatory cardioprotective effects in failure besides its hypoglycemic effect. However, the role of CANA myocarditis has not been elucidated. In this work, treatment markedly alleviated cardiac inflammation improved function experimental autoimmune (EAM) mice induced by α-myosin-heavy chain peptides. The expressions NLRP3 inflammasome...
Selective elimination of mitochondria by autophagy is a critical strategy for variety physiological processes, including development, cell-fate determination and stress response. Although several mechanisms have been identified as responsible selective degradation mitochondria, such the PINK1-PRKN/PARKIN- receptor-dependent pathways, aspects particularly principles underlying selection process remain obscure. Here, we addressed new in which dependent on organellar topology. We found that...
Adenocarcinoma of the bladder is a rare urinary carcinoma with limited therapy options due to lack molecular characterization. Here, we aimed reveal mutational and transcriptomic landscapes adenocarcinoma assess any relationship prognosis. Between February 2015 June 2021, total 23 patients were enrolled. These included 16 primary adenocarcinomas seven urachal adenocarcinoma. Whole exome sequencing (16 patients), whole genome bulk RNA (RNA-seq) (19 single-cell RNA-seq (5 patients) conducted...
The mechanisms of somatic cell reprogramming have been revealed at multiple levels. However, the lack tools to monitor different reactive oxygen species (ROS) has left their distinct signals and roles in unknown. We hypothesized that mitochondrial flashes (mitoflashes), recently identified spontaneous bursts superoxide signaling, play a role reprogramming. Here we show frequency mitoflashes transiently increases, accompanied by flash amplitude reduction, during early stages This transient...
Abstract B cells contribute to the development of dilated cardiomyopathy (DCM) by inducing myocyte injuries and myocardial fibrosis. Our recent research indicated that microRNA (miR) -185 participated in human B-cell activation. Thus, this study was aimed explore relationship between miR-185 DCM progression. Forty-one healthy volunteers fifty newly diagnosed patients were enrolled. The levels plasma miR-185, TNF-α secreting anti-heart autoantibody detected. We found mean significantly higher...
Abstract Background The majority of microbial fermentations are currently performed in the batch or fed-batch manner with high process complexity and huge water consumption. continuous production can contribute to green sustainable development fermentation industry. co-culture systems photo-autotrophic heterotrophic species play important roles establishing mode for bio-based chemicals production. Results In present paper, system Synechococcus elongates – Escherichia coli was established put...
Abstract The dorsoventral gradient of BMP signaling plays an essential role in embryonic patterning. Zinc Finger SWIM-Type Containing 4 ( zswim4 ) is expressed the Spemann-Mangold organizer at onset Xenopus gastrulation and then enriched developing neuroectoderm mid-gastrula stages. Knockdown or knockout causes ventralization. Overexpression decreases, whereas knockdown increases expression levels ventrolateral mesoderm marker genes. Mechanistically, ZSWIM4 attenuates signal by reducing...
Remodeled mitochondria are characteristic of pluripotent stem cells. However, a role for mitochondrial movement and distribution in pluripotency remains unknown. Here, we show that retrograde transport-mediated perinuclear clustering via dynein complex occurs at the early phase induction. Interestingly, this redistribution is regulated by Yamanaka factor OCT4 but not SOX2 or KLF4. This redistribution, which has effect on efficiency somatic cell reprogramming, also depends DRP1-mediated...
Myocardial injuries in viral myocarditis (VMC) are caused by infection and related autoimmune disorders. Recent studies suggest that IL-9 mediated both antimicrobial immune responses addition to allergic diseases. However, the role of VMC remains controversial uncertain. In this study, we infected Balb/c mice with Coxsackievirus B3 (CVB3), found was enriched blood hearts on days 5 7 after virus infection. Most secreted CD8 + T cells day CD4 myocardium. Further, knockout exacerbated cardiac...