A5046860509- Asthma and respiratory diseases
- DNA Repair Mechanisms
- SARS-CoV-2 and COVID-19 Research
- Computational Drug Discovery Methods
- Neonatal Respiratory Health Research
- Cystic Fibrosis Research Advances
- Transplantation: Methods and Outcomes
- Immune Cell Function and Interaction
- Respiratory viral infections research
- Pediatric health and respiratory diseases
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Mesenchymal stem cell research
- Allergic Rhinitis and Sensitization
- Tissue Engineering and Regenerative Medicine
- Pneumonia and Respiratory Infections
- Respiratory and Cough-Related Research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Fatty Acid Research and Health
- Mitochondrial Function and Pathology
- Genetic Neurodegenerative Diseases
- Immunodeficiency and Autoimmune Disorders
- Hematopoietic Stem Cell Transplantation
- MicroRNA in disease regulation
- Carcinogens and Genotoxicity Assessment
- Respiratory Support and Mechanisms
Goethe University Frankfurt
2016-2025
University Hospital Frankfurt
2015-2024
Klinik und Poliklinik für Kinder- und Jugendmedizin
2008-2024
Universität der Bundeswehr München
2021
The San Raffaele Telethon Institute for Gene Therapy
2021
San Raffaele University of Rome
2021
Zentrum für Kinderheilkunde
2013-2020
Goethe Institute
2015-2016
Children's Hospital
2015
Zittau/Görlitz University of Applied Sciences
2015
Natural killer (NK) cells are an important effector cell type for adoptive cancer immunotherapy. Similar to T cells, NK can be modified express chimeric antigen receptors (CARs) enhance antitumor activity, but experience with CAR-engineered and their clinical development is still limited. Here, we redirected continuously expanding clinically usable established human NK-92 the tumor-associated ErbB2 (HER2) antigen. Following GMP-compliant procedures, generated a stable clonal line expressing...
Abstract Mesenchymal stromal cells (MSC) provide a supportive cellular microenvironment and are able to maintain the self-renewal capacity of hematopoietic progenitor (HPC). Isolation procedures for MSC vary extensively, this may influence their biologic properties. In study, we have compared human isolated from bone marrow (BM) using two culture conditions, cord blood (CB), adipose tissue (AT). The ability long-term culture-initiating cell frequency primitive CD34+CD38− immunophenotype was...
Introduction: The German PID-NET registry was founded in 2009, serving as the first national of patients with primary immunodeficiencies (PID) Germany. It is part European Society for Immunodeficiencies (ESID) registry. purpose to gather data on epidemiology, diagnostic delay, diagnosis, and treatment PIDs. Methods: Clinical laboratory collected from 2,453 36 PID centres an online Data analysed software Stata® Excel. Results: minimum prevalence Germany 2.72 per 100,000 inhabitants. Among...
Abstract Acute myeloid leukemia (AML) is a malignant disorder derived from neoplastic progenitor cells characterized by abnormal proliferation and differentiation. Although novel therapeutics have recently been introduced, AML remains therapeutic challenge with insufficient cure rates. In the last years, immune-directed therapies such as chimeric antigen receptor (CAR)-T were which showed outstanding clinical activity against B-cell malignancies including acute lymphoblastic (ALL). However,...
Natural Killer (NK) cells are known for their high intrinsic cytotoxic capacity, and the possibility to be applied as 'off-the-shelf' product makes them highly attractive cell-based immunotherapies. In patients with multiple myeloma (MM), an elevated number of NK has been correlated higher overall-survival rate. However, cell function can impaired by upregulation inhibitory receptors, such immune checkpoint NKG2A. Here, we developed a CRISPR-Cas9-based gene editing protocol that allowed us...
Ataxia telangiectasia (AT) is a pleiotropic genetic disorder characterized by progressive neurodegeneration, especially of cerebellar Purkinje cells, immunodeficiency, increased incidence cancer, and premature aging. The disease caused functional inactivation the ATM (AT-mutated) gene product, which thought to act as sensor reactive oxygen species oxidative damage cellular macromolecules DNA. compound phenotype AT might thus be linked continuous state stress leading an increase programmed...
Reactive oxygen species (ROS) are important endogenous etiological agents for DNA damage, and ROS perform critical signaling functions in apoptosis, stress responses proliferation. The correlation between a lower incidence of cancer people who consume diet high naturally occurring antioxidants the observed increased cancerous tissues suggest that may be used chemoprevention. We tested this hypothesis by determining whether well-described nitroxide antioxidant, tempol...
Probiotics are perceived to exert beneficial effects in the prevention and treatment of allergic diseases.There conflicting data from studies as an impact on sensitization asthma.Our prospective double-blind study randomly assigned 131 children (6-24 months old) with at least two wheezing episodes a first-degree family history atopic disease 6 Lactobacillus rhamnosus (LGG, 10(10) colony forming units) or placebo. Atopic dermatitis asthma-related events (e.g. need inhalation, symptom-free...
The characterization of adipose-derived stromal/stem cells (ASCs) remains difficult due to the lack a definitive and unique cellular marker. Therefore, combination markers is necessary identify cells. No comprehensive analysis immunophenotype expanded plastic adherent ASCs has been published. aim this study was characterize general phenotype cultured further analyze subsets. were isolated from lipoaspirates patients undergoing cosmetic liposuction in standard cell culture. A done with BD...
To compare cytokines in undiluted vitreous of treatment-naïve patients with macular oedema without vitreomacular traction secondary to branch (BRVO), central (CRVO) and hemi-central (H-CRVO) retinal vein occlusion.Ninety-four (median age 72 years, 42 men) underwent an intravitreal combination therapy, including a single-site 23-gauge core vitrectomy the application bevacizumab dexamethasone due vision-decreasing oedema. Among these were 43 BRVO, 35 CRVO 16 hemi-CRVO, which distributed fresh...
In 2009, a federally funded clinical and research consortium (PID-NET, http://www.pid-net.org) established the first national registry for primary immunodeficiencies (PID) in Germany. The contains genetic information on PID patients is set up within framework of existing European Database Primary Immunodeficiencies, run by Society Immunodeficiencies. Following example other registries, central data entry clerk has been employed to support at participating centres. Regulations ethics...
We investigated the anti-inflammatory potential of n-3 polyunsaturated fatty acids (PUFA) on specific bronchial inflammation. Allergic asthmatics were challenged using a low-dose allergen provocation model.Our parallel double-blinded study randomly assigned 23 house dust mite-allergic (aged 22-29 years; 13 females, 10 males) to dietary supplementation with either an PUFA-enriched fat blend (0.69 g/day) or placebo for 5 weeks. After 3 weeks, patients daily low doses mite 2 Primary outcome...
Pre-emptive cancer immunotherapy by donor lymphocyte infusion (DLI) using cytokine-induced killer (CIK) cells may be beneficial to prevent relapse with a reduced risk of causing graft-versus-host-disease. CIK are heterogeneous effector cell population including T (CD3(+) CD56(-) ), natural (NK) (CD3(-) CD56(+) ) and (T-NK) that exhibit non-major histocompatibility complex (MHC)-restricted cytotoxicity generated ex vivo expansion peripheral blood mononuclear in the presence interferon...
Lung failure is responsible for significant morbidity and a frequent cause of death in ataxia-telangiectasia (A-T). Disturbance the redox balance alveolar epithelial cells must be considered as causal factor respiratory disease A-T. To investigate bronchoalveolar sensitivity to reactive oxygen species (ROS) ROS-induced DNA damage, we used bleomycin (BLM) induce experimental inflammation fibrotic changes Atm-deficient mouse model. BLM or saline was administered by oropharyngeal instillation...
Head and neck squamous cell carcinoma (HNSCC) is a highly heterogeneous aggressive tumor originating from the epithelial lining of upper aero-digestive tract accounting for 300,000 annual deaths worldwide due to failure current therapies. The natural killer group 2D receptor (NKG2D) on (NK) cells several T subsets plays an important role immunosurveillance HNSCC thus targeted by immune evasion strategies in particular shedding various NKG2D ligands (NKG2DLs). Based plasma samples 44...
A high incidence of thromboembolic events associated with mortality has been reported in severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) infections failure. The present study characterized post-transcriptional gene regulation by global microRNA (miRNA) expression relation to activated coagulation and inflammation 21 critically ill SARS-CoV-2 patients. cohort consisted patients moderate failure (n = 11) 10) at an stage (day 0-3) the later course disease (>7 days). All needed...
SUMMARY Highly reactive oxygen species (ROS) are involved in T-cell activation and the defense against environmental pathogens. An imbalance of ROS generation detoxifying scavenger enzymes could contribute to increased susceptibility cancer infections ataxia telangiectasia. We studied oxidative status, i.e. plasma total antioxidant capacity (TEAC), retinol, α-tocopherol, ubiquinol, number activated T cells 10 patients with telangiectasia (AT) compared age-matched healthy controls. As...