A5034721615- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- Lung Cancer Treatments and Mutations
- RNA Research and Splicing
- Colorectal Cancer Treatments and Studies
- MicroRNA in disease regulation
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- Lung Cancer Diagnosis and Treatment
- Glioma Diagnosis and Treatment
- Cancer-related molecular mechanisms research
- Plant Virus Research Studies
- BRCA gene mutations in cancer
- Cystic Fibrosis Research Advances
- HER2/EGFR in Cancer Research
- Nutrition, Genetics, and Disease
- Genetic factors in colorectal cancer
- Bioinformatics and Genomic Networks
- Mechanisms of cancer metastasis
- Angiogenesis and VEGF in Cancer
- Genomics and Chromatin Dynamics
- Neonatal Respiratory Health Research
- Tracheal and airway disorders
- Cancer Treatment and Pharmacology
- Cancer, Hypoxia, and Metabolism
Nicolaus Copernicus University
2014-2023
Collegium Medicum in Bydgoszcz
2012-2023
University of Bydgoszcz
2021
Northwestern University
2009-2019
University of North Carolina Health Care
2019
University of Kansas
2019
National Institutes of Health
2019
Leidos (United States)
2019
Leidos Biomedical Research Inc. (United States)
2019
Tufts University
2019
A compound that reduces the prevalence of perinucleolar compartment in cancer cells inhibits metastasis vivo.
// Karol Czubak 1 , Marzena Anna Lewandowska 2, 3 Katarzyna Klonowska Krzysztof Roszkowski 4, 5 Janusz Kowalewski 3, 6 Marek Figlerowicz Piotr Kozlowski European Centre for Bioinformatics and Genomics, Institute of Bioorganic Chemistry, Polish Academy Sciences, Poznan, Poland 2 Molecular Oncology Genetics Department, Innovative Medical Forum, The Franciszek Lukaszczyk Center, Bydgoszcz, Department Thoracic Surgery Tumors, Nicolaus Copernicus University, Torun, Collegium Medicum, 4...
The accurate detection of genetic variants such as single substitutions (IDH1/2, TERT), chromosomal abnormalities (CDKN2A, 1p/19q deletions, and EGFR amplifications), or promoter methylations (MGMT) is critical for glioma patient management, emphasized in the World Health Organization's (WHO's) most recent classification 2021 (WHO CNS5). purpose this study was to evaluate novel innovative methods determining IDH1/2 status context WHO CNS5. Multiple biomarkers were simultaneously screened...
A growing body of evidence indicates that miRNAs may either drive or suppress oncogenesis. However, little is known about somatic mutations in miRNA genes. To determine the frequency and potential consequences gene mutations, we analyzed whole exome sequencing datasets 569 lung adenocarcinoma (LUAD) 597 squamous cell carcinoma (LUSC) samples generated The Cancer Genome Atlas (TCGA) project. Altogether, identified 1091 sequence variants affecting 522 different genes showed half all cancers...
Genomic variations deep in the intronic regions of pre-mRNA molecules are increasingly reported to affect splicing events. However, there is no general explanation why apparently similar may have either effect on or cause significant alterations. In this work we examined structural architecture pseudoexons previously described ATM and CFTR patients. The case derives from deletion a repressor element characterized by an aberrant 5′ss selection despite presence better alternatives. pseudoexon...
Testing for the epidermal growth factor receptor (EGFR) gene mutations requires considerable multidisciplinary experience of clinicians (for appropriate patient selection), pathologists selection cytological or histological material) and geneticists performing reporting reliable molecular tests). We present our on efficacy routine EGFR testing in various types tumor samples frequency a large series Polish non-small cell lung cancer (NSCLC) patients.Deletions exon 19 substitution L858R 21...
Abstract The overall prevalence of germline BRCA 1/2 mutations is estimated between 11% and 15% all ovarian cancers. Individuals with alterations treated the PARP 1 inhibitors ( iPARP 1) tend to respond better than patients wild‐type 1/2. Additionally, also somatic induce sensitivity 1. Therefore, detection both required for effective treatment. aim this study was identify frequency spectrum in a group Polish serous carcinoma. In total, 100 formalin‐fixed paraffin‐embedded FFPE ) carcinoma...
There is a growing interest in the genetic variation of noncoding genomic elements, including miRNAs, and several mutations miRNA genes implicated human diseases, cancer, have already been detected. However, lack dedicated analytical tools severely hampers progress this area. In study, we developed whole-miRNome sequencing (WMS), which enables targeted all (n~2000) 28 biogenesis genes. Herein, by almost 600 samples, ~300 tumor/normal pairs samples from different cancer types, identified...
Background/Aims: Mutations in the CFTR gene cause Cystic Fibrosis (CF) most common life-threatening autosomal recessive disease affecting Caucasians. We identified a mutation (c.120del23) abolishing normal translation initiation codon, which occurs two Portuguese CF patients. This study aims at functionally characterizing effect of this novel mutation. Methods: RNA and protein techniques were applied to both native tissues from patients recombinant cells expressing constructs determine...
The low levels of CFTR gene expression and paucity protein in human airway epithelial cells are not easily reconciled with the pivotal role lung cystic fibrosis pathology. Previous data suggested that regulatory mechanisms controlling might be different epithelium comparison to intestinal where mRNA is much more abundant. Here we examine chromatin structure modification across locus primary tracheal (HTE) bronchial (NHBE) cell lines including 16HBE14o- Calu3. We identify regions open appear...
The rapid development of molecular biology techniques allows for the introduction real-time polymerase chain reaction (PCR) methods with a limit mutation detection at 1 % in background wild-type DNA. Analysis KRAS mutations codons 12, 13, and 61, together analysis BRAF codon 600, are predictive biomarkers anti-epidermal growth factor receptor (EGFR) treatment colorectal cancer. Our aim was to compare PCR using DNA isolated from tissue samples previously evaluated presence tumor cells...
Lung cancer is the leading cause of cancer-related death worldwide. Recent progress in lung diagnosis and treatment has been achieved due to a better understanding molecular mechanisms disease identification biomarkers that allow more specific treatments. One best known examples personalized therapy use tyrosine kinase inhibitors, such as gefitinib erlotinib, for successful non-small-cell patients selected based on EGFR mutations. Therefore, reliable detection mutations critical application...
The epidermal growth factor receptor (EGFR) mutation status in the tyrosine kinase domain is known to be a predictor of response gefitinib or erlotinib lung cancer; thus, non-surgical procedure tumor specimen collection critical for analysis. aim present study was analyze EGFR, KRAS and BRAF limited cytological material. To best our knowledge, this first time that quantitative scale cells percentage material were evaluated at early stages pathomorphological qualification Our results revealed...
A. Slusarczyk1, R. Kamath1, C. Wang1, D. Anchel2, Pollock1, M.A. Lewandowska1, T. Fitzpatrick1, D.P. Bazett-Jones2 and S. Huang1⇓ Department of Cell Molecular Biology, Northwestern University Feinberg School Medicine, Chicago, Illinois 60614; Biochemistry, Toronto, Canada M5G 1L7 Correspondence: s-huang2{at}northwestern.edu
IDH1 (isocitrate dehydrogenase 1) is a potential biomarker and drug target. Genomic epigenetic data on astrocytoma have demonstrated that the mutation sufficient to establish glioma hypermethylator phenotype. Furthermore, recent studies also indicated mutant inhibitor induced demethylation of histone H3K9me3 expression genes associated with gliogenic differentiation. As presence p.R132H in gene seems be more powerful prognostic marker than O(6)-methylguanine-DNA methyltransferase promoter...
We have previously reported a natural GTAA deletion within an intronic splicing processing element (ISPE) of the ataxia telangiectasia mutated (ATM) gene that disrupts non-canonical U1 snRNP interaction and activates excision upstream portion intron. The resulting pre-mRNA intermediate is then processed to cryptic exon, whose aberrant inclusion in final mRNA responsible for telangiectasia. show here last 40 bases downstream antisense Alu repeat are required activation exon by ISPE deletion....
The perinucleolar compartment (PNC) forms in cancer cells and is highly enriched with a subset of polymerase III RNAs RNA-binding proteins. Here we report that PNC components mitochondrial RNA-processing (MRP) RNA, pyrimidine tract-binding protein (PTB), CUG-binding (CUGBP) interact vivo, as demonstrated by coimmunoprecipitation RNA pull-down experiments. Glycerol gradient analyses show this complex large sediments at different fraction from known MRP RNA-containing complexes, the...