A5115600813- Nanofabrication and Lithography Techniques
- Microbial Metabolic Engineering and Bioproduction
- Bacteriophages and microbial interactions
- Electrowetting and Microfluidic Technologies
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Legume Nitrogen Fixing Symbiosis
- Enzyme Production and Characterization
- Microbial Community Ecology and Physiology
- Monoclonal and Polyclonal Antibodies Research
- Fungal and yeast genetics research
- Genomics and Phylogenetic Studies
- Nanowire Synthesis and Applications
- Advanced Biosensing Techniques and Applications
- Innovative Microfluidic and Catalytic Techniques Innovation
- Plant Pathogenic Bacteria Studies
- Electromagnetic Fields and Biological Effects
- Glycosylation and Glycoproteins Research
- Marine Toxins and Detection Methods
- Polysaccharides and Plant Cell Walls
- Single-cell and spatial transcriptomics
- Mesenchymal stem cell research
- AI in cancer detection
- Biofuel production and bioconversion
- Escherichia coli research studies
Centre National de la Recherche Scientifique
2007-2018
Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés
2004-2018
Université de Toulouse
2009-2018
Institut National des Sciences Appliquées de Toulouse
2003-2018
Academia Nacional de Medicina
2017
Université Toulouse III - Paul Sabatier
2009-2016
Institut National Polytechnique de Toulouse
2009
Genopole (France)
2003-2009
Rationale: In addition to their well-known function as antibody-producing cells, B lymphocytes can markedly influence the course of infectious or noninfectious diseases via antibody-independent mechanisms. tuberculosis (TB), cells accumulate in lungs, yet functional contribution host response remains poorly understood.Objectives: To document role TB an unbiased manner.Methods: We generated transcriptome isolated from Mycobacterium (Mtb)-infected mice and validated identified key pathways...
Article16 November 2018Open Access Transparent process A conserved mechanism drives partition complex assembly on bacterial chromosomes and plasmids Roxanne E Debaugny Laboratoire de Microbiologie et Génétique Moléculaires, Centre Biologie Intégrative (CBI), National la Recherche Scientifique (CNRS), Université Toulouse, UPS, France Search for more papers by this author Aurore Sanchez Jérôme Rech Delphine Labourdette LISBP, CNRS, INRA, INSA, Dorignac Charles Coulomb, CNRS-Université...
Arrays of silicon-based microcantilevers with properly designed passivated aluminum electrodes have been used to generate microarrays by depositing microspots biological samples using a direct contact deposition technique. The approach proposed here can be compared the dip-pen technique but noticeable difference that electrostatic fields are generated onto cantilevers increase height liquid rise on cantilever surface when dipping them into deposited. Both electrowetting through reduction...
Microorganisms constitute a reservoir of enzymes involved in environmental carbon cycling and degradation plant polysaccharides through their production vast variety Glycoside Hydrolases (GH). The CAZyChip was developed to allow rapid characterization at transcriptomic level these GHs identify acting on hydrolysis or glycans.This DNA biochip contains the signature 55,220 bacterial available CAZy database. Probes were designed using two softwares, microarrays directly synthesized situ ink-jet...
The dermal Panniculus carnosus (PC) muscle is important for wound contraction in lower mammals and represents an interesting model of regeneration due to its high cell turnover. resident satellite cells (the bona fide stem cells) remain poorly characterized. Here we analyzed PC with regard developmental origin purported function. Lineage tracing shows that they originate Myf5+, Pax3/Pax7+ populations. Skin wounding increased myofiber turnover, the progeny being involved but no detectable...
Abstract We report results of a study the effects strong static (up to 16 T for 8 h) and pulsed 55 single‐shot 4 × 20 repeated shots) magnetic fields on Saccharomyces cerevisiae cultures in exponential phase growth. In contrast previous reports restricted only limited number cellular parameters, we have examined wide variety processes: genome‐scale gene expression, proteome profile, cell viability, morphology, growth, metabolic fermentation activity after field exposure. None these...
The segregation of plasmid F Escherichia coli is highly reliable. Sop partition locus, responsible for this stable maintenance, composed two genes, sopA and sopB a centromere, sopC , consisting 12 direct repeats 43 bp. Each repeat carries 16-bp inverted motif to which SopB binds form nucleoprotein assembly called the complex. A database search sequences closely related revealed unexpected features that appeared conserved. We have investigated requirements specific SopB– interactions using...
Personalized medicine has become a priority in breast cancer patient management. In addition to the routinely used clinicopathological characteristics, clinicians will have face an increasing amount of data derived from tumor molecular profiling. The aims this study were develop new gene selection method based on fuzzy logic and classification algorithm, validate signatures obtained cohorts. We analyzed four published expression datasets for carcinomas. identified best discriminating genes...
ABSTRACT Biocatalysts are essential for the development of bioprocesses efficient plant biomass degradation. Previously, a metagenomic clone containing DNA from termite gut microbiota was pinpointed in functional screening that revealed presence arabinofuranosidase activity. Subsequent genetic and bioinformatic analysis fragment belonged to member genus Bacteroides encoded 19 open reading frames (ORFs), annotation suggested hypothetical transporter regulator proteins others involved...
The isoprenoid pathway in yeasts is important not only for sterol biosynthesis but also the production of nonsterol molecules, deriving from farnesyl diphosphate (FPP), implicated N-glycosylation and heme ubiquinones. FPP formed mevalonate a reaction catalyzed by synthase (Erg20p). In order to investigate regulation Erg20p Saccharomyces cerevisiae, we searched its protein partners using two-hybrid screen, identified five interacting proteins, among them Yta7p. Subsequently, showed that Yta7p...
Bacterial centromeres-also called parS, are cis-acting DNA sequences which, together with the proteins ParA and ParB, involved in segregation of chromosomes plasmids. The specific binding ParB to parS nucleates assembly a large ParB/DNA complex from which ParA-the motor protein, segregates sister replicons. Closely related families partition systems, Bsr, were identified on plasmids multi-chromosomal bacterium Burkholderia cenocepacia other species order Burkholeriales. centromeres Bsr 16 bp...
Arrays of silicon-based microcantilevers with passivated aluminum electrodes properly designed have been used to generate microarrays by depositing microspots biological samples using a direct contact deposition technique. Using this approach, single loading, arrays more than hundred spots, from the femtoliter picoliter range, containing fluorescent-labelled oligonucleotides (15 mers) and proteins were directly patterned on glass slide. The aim here was check whether our system could be for...
Le premier decryptage du genome humain a necessite plus de dix ans travail et un investissement pres 3 milliards dollars. Depuis, plusieurs societes se sont lancees dans la course, avec pour objectif l'analyse d'un le rapidement possible cout inferieur 1000 $ par genome. Ces sequenceurs nouvelle generation ont revolutionne les analyses en genomique. Avec champ d'application tres etendu, ces methodes deviennent incontournables modifie facon dont scientifiques envisagent etudes Dans cet...
Summary Chromosome and plasmid segregation in bacteria are mostly driven by ParABS systems. These DNA partitioning machineries rely on large nucleoprotein complexes assembled centromere sites ( parS ). However, the mechanism of how a few -bound ParB proteins nucleate formation highly concentrated clusters remains unclear despite several proposed physico-mathematical models. We discriminated between these different models varying some key parameters vivo using F partition system. found that...