A5101616698- Hematopoietic Stem Cell Transplantation
- Mesenchymal stem cell research
- Immune cells in cancer
- Acute Myeloid Leukemia Research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Immunodeficiency and Autoimmune Disorders
- Digital Imaging for Blood Diseases
- Neonatal Respiratory Health Research
- Renal and related cancers
- AI in cancer detection
- Cell Image Analysis Techniques
- Pluripotent Stem Cells Research
- CAR-T cell therapy research
- RNA Interference and Gene Delivery
- Renal Diseases and Glomerulopathies
- Angiogenesis and VEGF in Cancer
- Genetic and Kidney Cyst Diseases
- Platelet Disorders and Treatments
- Chemokine receptors and signaling
- Multiple Myeloma Research and Treatments
- Cancer Research and Treatments
- Virus-based gene therapy research
- Cancer Cells and Metastasis
Kansai Medical University
2013-2024
The identification of human CD34-negative (CD34−) hematopoietic stem cells (HSCs) provides a new concept for the hierarchy in HSC compartment. Previous studies demonstrated that CD34− severe combined immunodeficiency (SCID)-repopulating (SRCs) are distinct class primitive HSCs comparison to well-characterized CD34+CD38− SRCs. However, purification level rare SRCs 18 lineage marker-negative (Lin−) (1/1000) is still very low compared with (1/40). As mouse, it will be necessary identify useful...
Abstract We previously identified CD34-negative (CD34 − ) severe combined immunodeficiency (SCID)-repopulating cells as primitive hematopoietic stem (HSCs) in human cord blood. In this study, we develop a prospective ultra-high-resolution purification method by applying two positive markers, CD133 and GPI-80. Using method, succeed purifying single long-term repopulating CD34 HSCs with self-renewing capability residing at the apex of HSC hierarchy from blood, evidenced single-cell-initiated...
Highlights•The percentages of CD271+SSEA-4+ MSCs (DPMSCs) in bone tissue decline with age•DPMSCs may accelerate cellular senescence through TGF-β2•TGF-β can change the balance adipogenesis and osteogenesis DPMSCs•The aging phenomena possibly be reversed by anti-TGF-β antibodiesSummaryIt is generally thought that proliferative capacity differentiation potential somatic stem cells, including mesenchymal stromal/stem cells (MSCs) hematopoietic age. We investigated effects on human bone-derived...
Summary Monoclonal antibody (mAb) drugs are desirable for the improvement of multiple myeloma (MM) treatment. In this study, we found first time that CD48 was highly expressed on MM plasma cells. 22 out 24 patients, more than 90% cells at significantly higher levels it normal lymphocytes and monocytes. only weakly some CD34 + haematopoietic stem/progenitor cells, not erythrocytes or platelets. We next examined whether could serve as a target antigen mAb therapy against MM. A newly generated...
Although c-kit is expressed highly on murine hematopoietic stem cells (HSCs) and essential for bone marrow (BM) hematopoiesis, the significance of high level expression HSCs was not well determined. We show here that CD150(+) CD48(-) Lineage(-) Sca-1(+) c-kit(+) in adult BM are distributed within range roughly a 20-fold difference c-kit, density correlates with cycling status HSC population. This predisposition more evident mice older than 30 weeks. The G(0) phase express lower both cell...
The use of dendritic cells (DC) to prime tumor-associated antigen-specific T-cell responses provides a promising approach cancer immunotherapy. Embryonic stem (ESC) and induced pluripotent (iPSC) can differentiate into functional DCs, thus providing an unlimited source DCs. However, the previously established methods generating practical volumes DCs from (PSC) require large number PSCs at start differentiation culture. In this study, we generated mouse proliferating myeloid (pMC) as...
Murine bone marrow (BM)-derived very small embryonic-like stem cells (BM VSELs), defined by a lineage-negative (Lin(-)), CD45-negative (CD45(-)), Sca-1-positive (Sca-1(+)) immunophenotype, were previously reported as postnatal pluripotent (SCs). We developed highly efficient method for isolating Lin(-)CD45(-)Sca-1(+) using enzymatic treatment of murine bone. designated these bone-derived VSELs (BD VSELs). The incidences BM in the BM-derived nucleated and that BD 0.002% 0.15%, respectively....
CD1d-restricted invariant NKT (iNKT) cells play crucial roles in various types of immune responses, including autoimmune diseases, infectious diseases and tumor surveillance. The mechanisms underlying their adjuvant functions are well understood. Nevertheless, although IL-4 IL-10 production characterize iNKT able to prevent or ameliorate some inflammatory conditions, the precise by which exert regulatory function remain elusive. This study demonstrates that activation human specific ligand...
OECs (outgrowth endothelial cells), also known as late-EPCs (late-endothelial progenitor have a high proliferation potential in addition to vitro tube formation capability. In ischaemic animal models, injected were integrated into regenerating blood vessels and improved neovascularization. Previous reports demonstrated the expression of CXCL8 be up-regulated tissues. It has been documented that stimulates angiogenic activity mature ECs (endothelial cells). Therefore, it suggested plays an...
Citrin deficiency is a congenital secondary urea cycle disorder lacking useful disease models for effective treatment development. In this study, human induced pluripotent stem cells (iPSCs) were generated from two patients with citrin and differentiated into hepatocyte-like (HLCs). Citrin-deficient HLCs produced albumin liver-specific markers but completely lacked protein expressed argininosuccinate synthase only weakly. addition, ammonia concentrations in medium cultured citrin-deficient...
In the murine hematopoietic stem cell (HSC) compartment, thrombopoietin (THPO)/MPL (THPO receptor) signaling plays an important role in maintenance of adult quiescent HSCs. However, THPO/MPL human primitive HSC compartment has not yet been elucidated. We have identified very cord blood (CB)-derived CD34 – severe combined immunodeficiency (SCID)-repopulating cells (SRCs) using intra-bone marrow injection method. this study, we investigated roles MPL expression compartment. The SRC activities...
It is well documented that specialized mesenchymal stem/stromal cells (MSCs) constitute the hematopoietic stem cell (HSC) niche in bone marrow (BM), and these MSCs support/maintain HSCs an undifferentiated state. A number of studies have demonstrated BM-derived (BM-MSCs) can support vitro. However, it remains unclear whether nonhematopoietic tissue-derived MSC-like cells, such as dental pulp (DPSCs), ability to HSCs. In this study, we prospectively isolated DPSCs from mouse mandibular...
The parathyroid gland plays an essential role in mineral and bone metabolism. Cultivation of physiological human cells has yet to be established the method by which differentiate from pluripotent stem remains uncertain. Therefore, it been hard clarify mechanisms underlying onset disorders, such as hyperparathyroidism. In this study, we developed a new cell differentiation induced (iPS) cells. Parathyroid occurred accordance with embryologic development. Differentiated cells, expressed...