A5101702984- Enzyme Production and Characterization
- Polysaccharides and Plant Cell Walls
- Polysaccharides Composition and Applications
- Proteins in Food Systems
- Blood properties and coagulation
- Meat and Animal Product Quality
- Protein Hydrolysis and Bioactive Peptides
- Biofuel production and bioconversion
- Phytase and its Applications
- Food composition and properties
- Microbial Metabolites in Food Biotechnology
- Biochemical and Molecular Research
- Advanced Cellulose Research Studies
- Digestive system and related health
- Polyamine Metabolism and Applications
- Biopolymer Synthesis and Applications
- Cancer, Lipids, and Metabolism
- Protein purification and stability
- Nanoparticle-Based Drug Delivery
- Probiotics and Fermented Foods
- Cancer Research and Treatments
- RNA Interference and Gene Delivery
- Lipoproteins and Cardiovascular Health
- Dye analysis and toxicity
- Enzyme Catalysis and Immobilization
East China Normal University
2015-2025
Pharmaceutical Biotechnology (Czechia)
2022
Nanjing University
2022
State Key Laboratory of Pharmaceutical Biotechnology
2022
Wannan Medical College
2022
Nanjing Medical University
2022
Nanjing Forestry University
2022
State Key Laboratory of Natural Medicine
2019
China Pharmaceutical University
2019
Shanghai Textile Holdings (China)
2012
Two unprecedented lindenane sesquiterpene dimers, chlotrichenes A (1) and B (2), were obtained from Chloranthus holostegius var. trichoneurus. Their structures characterized by NMR, ECD, X-ray diffraction analysis. They possess a new type of spirocarboncyclic dimeric framework formed endo-Diels–Alder reaction, the structure 1 features unique 3/5/6/6/6/6/5/3-fused octacyclic skeleton subsequently plausible epoxidation–cyclization reactions 2. Compound 2 showed synergetic cytotoxicity with DOX...
The lack of fat in yogurt can lead to alterations taste and whey separation, reducing consumer acceptance. In this study, the feasibility enhancing quality skim milk through a combination transglutaminase (TG) protein-glutaminase (PG) was investigated. TG PG resulted simultaneous cross-linking deamidated casein micelles, with deamidation taking priority over cross-linking, leading higher solubility lower turbidity proteins compared alone. When 0.06 U/mL 0.03 were added, firmness viscosity...
This research investigates the influence of inorganic salts on curdlan production, gel strength, molecular weight (Mw), and texture properties, to provide insights into optimizing fermentation conditions. Five different were individually incorporated medium assess their impact. The results showed that FeCl3 significantly reduced yield, Mw, quality, indicating its unsuitability for fermentation. FeSO4 at 0.01% enhanced such as springiness chewiness, though higher concentrations had adverse...
α-Glucosidase is an essential enzyme widely used in the food and biotechnology industries for production of isomaltose oligosaccharides (IMOs). In this study, Aspergillus niger ASP004 was subjected to two rounds nitrosoguanidine (NTG) mutagenesis, yielding a high-producing strain, NTG-II-3-64, with 3.7-fold increase activity under optimized fermentation conditions. The purified through ultrafiltration, ethanol precipitation, DEAE ion-exchange, gel filtration chromatography, resulting...
Protein glutaminase can catalyze the hydrolysis of glutamine residues in plant proteins into glutamic acid to enhance functional properties. However, yield PG Chryseobacterium proteolyticum was too low be used industrial production. In this study, C. TM1040 increased by 121%, up 7.30 U/mL a 15L fermenter after medium optimization. Subsequently, purified obtained cation exchange chromatography (CEX) coupled with hydrophobic interaction (HIC). The degree deamidation (DD) wheat gluten 87.11%,...
Protein glutaminase (PG), originating from Chryseobacterium proteolyticum, can catalyze the deamidation of glutamine residues in plant proteins into glutamic acid, thus enhancing its functional properties. However, low yield PG limits industrial production. In this study, C. proteolyticum TM1040 increased by 121 %, up to 7.30 U/mL a 15 L fermenter after medium optimization. Subsequently, purified was obtained cation exchange chromatography (CEX) coupled with hydrophobic interaction (HIC)....
Background: Lecithin cholesterol acyltransferase (LCAT) is an important enzyme responsible for free (FC) esterification, which critical high density lipoprotein (HDL) maturation and the completion of reverse transport (RCT) process. Plasma LCAT activity concentration showed various patterns under different physiological pathological conditions. Research on has grown rapidly over past 50 years, but there are no bibliometric studies summarizing this field as a whole. This study aimed to use...
Recently, an enzyme named protein-glutaminase (PG) has been identified as a new type of with significant potential for deamidation food proteins. The is shown to be expressed pre-pro-protein putative signal peptide 21 amino acids, pro-sequence 114 and mature PG 185 acids. microbial specifically catalyzes proteins without protein hydrolysis pretreatment only reacts glutamine residues in the side-chains or long peptides. All these attributes suggest that it great industrial applications....
Protein-glutaminase (PG), a deamidation enzyme commercially derived from Chryseobacterium proteolyticum, is used to improve the solubility and other functional properties of food proteins. In this study, new PG-producing strain, cucumeris ZYF120413-7, was isolated soil, it had high PG yield short culture time. It gave maximum activity with 0.557 U/ml on Cbz-Gln-Gly after 12 h culture, indicating that more suitable for production. The recovery purification fold were 32.95% 161.95-fold,...
Poly(γ-glutamic acid)-based targeted drug delivery system (PAMCN) targeting transmembrane metalloprotease aminopeptidase-N (APN/CD13) was fabricated and evaluated for the enhancement of efficiency cytotoxicity. The cisplatin (CDDP) loading content PAMCN about 36 ± 5% showed a sustainable release profile with half-maximal time (t1/2) 23 h. average size 132 18 nm determined by light scattering (LS) 158 67 atomic force microscopy (AFM). Flow cytometry fluorescence microscope analysis that...
The large-scale production of soybean proteins results in the generation a significant volume wastewater, containing substantial amount valuable β-amylase. β-amylase enzyme was purified from whey wastewater using three-step process, including alcohol precipitation, ion-exchange chromatography, and gel filtration chromatography. specific activity 29,700 U/mg, with an recovery 17.3% purification fold 16.5. had molecular mass around 56 kDa isoelectric point (pI) value 4.8. exhibited optimal at...
Curdlan has wide potential application in the food and biomedical fields due to its unique thermal gel biological activity. This study investigated effect of six sugars including glucose, fructose, lactose, maltose, sucrose xylose as carbon sources on production properties curdlan using Agrobacterium sp. DH-2. The maximum (38.1 g/L 37.4 g/L, respectively) yield (0.58 g curdlan/g 0.53 were achieved by followed lactose xylose. Scanning electron micrographs showed that surface cells was smooth...