A5016498366- Renal and related cancers
- Renal Diseases and Glomerulopathies
- Pluripotent Stem Cells Research
- Climate Change and Health Impacts
- Organ Donation and Transplantation
- Chronic Kidney Disease and Diabetes
- Biomedical Research and Pathophysiology
- Genetic and Kidney Cyst Diseases
- Dialysis and Renal Disease Management
- Acute Kidney Injury Research
- Renal cell carcinoma treatment
- Tissue Engineering and Regenerative Medicine
- Climate Change and Geoengineering
- CRISPR and Genetic Engineering
- Epigenetics and DNA Methylation
- Biomedical Ethics and Regulation
- Reproductive Biology and Fertility
- Pancreatic function and diabetes
- Cardiac tumors and thrombi
- Hippo pathway signaling and YAP/TAZ
- Global Health Care Issues
- Healthcare cost, quality, practices
- Erythrocyte Function and Pathophysiology
- Inflammasome and immune disorders
- Aortic Thrombus and Embolism
University of Florence
2018-2025
McGill University Health Centre
2024
RELX Group (United States)
2019-2023
Conference Board
2023
Meyer Children's Hospital
2018-2022
Ludwig-Maximilians-Universität München
2022
University of Turin
2010-2018
Cornell University
2016-2017
University College Dublin
2016
Biotechnology Research Center
2013
Acute kidney injury (AKI) is considered largely reversible based on the capacity of surviving tubular cells to dedifferentiate and replace lost via cell division. Here we show by tracking individual in conditional Pax8/Confetti mice that function recovered after AKI despite substantial loss. Cell cycle ploidy analysis upon Pax8/FUCCI2aR human biopsies identify endocycle-mediated hypertrophy cells. By contrast, a small subset Pax2+ progenitors enriches higher stress resistance clonal...
Acute tissue injury causes DNA damage and repair processes involving increased cell mitosis polyploidization, leading to function alterations that may potentially drive cancer development. Here, we show acute kidney (AKI) the risk for papillary renal carcinoma (pRCC) development tumor relapse in humans as confirmed by data collected from several single-center multicentric studies. Lineage tracing of tubular epithelial cells (TECs) after AKI induction long-term follow-up mice showed...
Abstract Acute kidney injury (AKI) is frequent, often fatal and, for lack of specific therapies, can leave survivors with chronic disease (CKD). We characterize the distribution tubular cells (TC) undergoing polyploidy along AKI by DNA content analysis and single cell RNA-sequencing. Furthermore, we study functional roles polyploidization using transgenic models drug interventions. identify YAP1-driven TC outside site as a rapid way to sustain residual function early during AKI. This...
Significance Statement To optimize the diagnosis of genetic kidney disorders in a cost-effective manner, we developed workflow based on referral criteria for in-person evaluation at tertiary center, whole-exome sequencing, reverse phenotyping, and multidisciplinary board analysis. This reached diagnostic rate 67%, with 48% confirming 19% modifying suspected clinical diagnosis. We obtained 64% children 70% adults. A modeled cost analysis demonstrated that early testing saves 20% costs per...
Raglianti, Valentina; Angelotti, Maria Lucia; De Chiara, Letizia; Allinovi, Marco; Cirillo, Luigi; Montero, Anna Manonelles; Cruzado, Josep Maria; Melica, Elena; Antonelli, Giulia; Conte, Carolina; Peired, Julie; Lasagni, Laura; Lange-Sperandio, Bärbel; Anders, Hans-Joachim; Becherucci, Francesca; Lazzeri, Mazzinghi, Benedetta; Romagnani, Paola Author Information
Crescentic glomerulonephritis is characterized by vascular necrosis and parietal epithelial cell hyperplasia in the space surrounding glomerulus, resulting formation of crescents. Little known about molecular mechanisms driving this process. Inducing crescentic two Pax2Cre reporter mouse models revealed that crescents derive from clonal expansion single immature cells. Preemptive delayed histone deacetylase inhibition with panobinostat, a drug used to treat hematopoietic stem disorders,...
Tubular epithelial cells (TC) compose the majority of kidney parenchyma and play fundamental roles in maintaining homeostasis. Like other tissues, mostly immature TC with progenitor capabilities are able to replace lost during injury via clonal expansion differentiation. In contrast, differentiated lack this capacity. However, as is frequently exposed toxic injuries, evolution positively selected a response program that endows maintain residual function injury. Recently, we others have...
Key Points A multinational survey of health care professionals on the kidney impacts climate change and environmental burden was conducted. Most participants reported knowledge gaps high level concern these interconnected issues. Only a minority report personal or organizational initiatives in environmentally sustainable care; this did not vary by country income level. Background Given threat to significant effect care, calls are increasing for organizations champion advocacy care. Yet,...
Cholesterol crystal embolism (CCE) implies immunothrombosis, tissue necrosis, and organ failure but no specific treatments are available. As CCE involves complement activation, we speculated that inhibitors of the C5a/C5aR axis would be sufficient to attenuate consequences like with systemic vasculitis. microcrystal injection into kidney artery wildtype mice initiated intra-kidney immunothrombosis within a few hours followed by sudden drop glomerular filtration rate ischemic necrosis after...
Spermatogonial stem cells reside in specific niches within seminiferous tubules and continuously generate differentiating daughter for production of spermatozoa. Although spermatogonial are unipotent, these able to spontaneously convert germline cell-derived pluripotent (GPSCs) vitro. GPSCs have many properties embryonic highly plastic, but their therapeutic potential tissue regeneration has not been fully explored. Using a novel renal epithelial differentiation protocol, we obtained...
The NLRP3 inflammasome integrates several danger signals into the activation of innate immunity and inflammation by secreting IL-1β IL-18. Most published data relate to in immune cells, but some reports claim similar roles parenchymal, namely epithelial, cells. For example, podocytes, epithelial cells critical for maintenance kidney filtration, have been reported express release IL-β diabetic disease, contributing filtration barrier dysfunction injury. We questioned this hence performed...
Podocytes and podocyte progenitors are interdependent components of the kidney's glomerular structure, with podocytes forming filtration barrier being key players in regeneration during pathophysiological processes. Both cell types subjected to constant mechanical forces, whose alterations can initiate podocytopathy worsen injury. Despite this, specific mechanosensors mechanotransduction pathways involved their response cues remain only partially explored. We used transcriptomics,...
Polyploidization of tubular cells (TC) is triggered by acute kidney injury (AKI) to allow survival in the early phase after AKI, but long run promotes fibrosis and AKI-chronic disease (CKD) transition. The molecular mechanism governing link between polyploid TC remains be clarified. In this study, we demonstrate that immediately expression cell cycle markers mostly identifies a population DNA-damaged TC. Using transgenic mouse models single-cell RNA sequencing show that, unlike diploid TC,...
Mesenchymal stem cells hold great promise for regenerative medicine as they can be easily isolated from different sources such adipose tissue, bone marrow, and umbilical cord blood. Spontaneously arising pluripotent obtained in culture murine spermatogonial (SSCs), while the pluripotency of human counterpart remains a matter debate. Recent gene expression profiling studies have demonstrated that embryonic cell- (ESC-) like testis are indeed closer to mesenchymal (MSCs) than cells. Here, we...
Acute organ injury, such as acute kidney injury (AKI) and disease (AKD), are major causes of morbidity mortality worldwide. Hyperuricemia (HU) is common in patients with impaired function but the impact asymptomatic HU on different phases AKI/AKD incompletely understood. We hypothesized that would attenuate AKD because soluble, contrast to crystalline, uric acid (sUA) can sterile inflammation. In vitro, 10 mg/dL sUA decreased reactive oxygen species interleukin-6 production macrophages,...