A5012198018- Cellular Mechanics and Interactions
- 3D Printing in Biomedical Research
- Microfluidic and Bio-sensing Technologies
- Force Microscopy Techniques and Applications
- Optical measurement and interference techniques
- Cell Adhesion Molecules Research
- Image Processing Techniques and Applications
- Graphene research and applications
- Bone Tissue Engineering Materials
- Microfluidic and Capillary Electrophoresis Applications
- Advanced Vision and Imaging
- Tendon Structure and Treatment
- T-cell and B-cell Immunology
- Graphene and Nanomaterials Applications
- Advanced Measurement and Metrology Techniques
- Advanced MEMS and NEMS Technologies
- Immunotherapy and Immune Responses
- Electrospun Nanofibers in Biomedical Applications
- Nanoparticle-Based Drug Delivery
- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- Polymer Surface Interaction Studies
- Acoustic Wave Resonator Technologies
- Nanopore and Nanochannel Transport Studies
- Mesenchymal stem cell research
Peking University
2016-2025
University of Chinese Academy of Sciences
2021-2025
Wenzhou University
2025
State Key Laboratory of Turbulence and Complex Systems
2015-2022
Zhejiang Lab
2021-2022
Suzhou Institute of Biomedical Engineering and Technology
2021
Chinese Academy of Sciences
2021
Tsinghua University
2017
Center for Interdisciplinary Studies
2017
State Key Laboratory of Artificial Microstructure and Mesoscopic Physics
2010
Abstract Cell therapeutics hold tremendous regenerative potential and the therapeutic effect depends on effective delivery of cells. However, current cell carriers with unsuitable cytocompatibility topological structure demonstrate poor viability during injection. Therefore, porous shape‐memory cryogel microspheres (CMS) are prepared from methacrylated gelatin (GelMA) by combining an emulsion technique gradient‐cooling cryogelation. Pore sizes CMS adjusted via procedure, optimized pore size...
Exosomes derived from mesenchymal stem cells (MSCs) have demonstrated regenerative potential for cell-free bone tissue engineering, nevertheless, certain challenges, including the confined therapeutic potency of exosomes and ineffective delivery method, are still persisted. Here, we confirmed that hypoxic precondition could induce enhanced secretion human exfoliated deciduous teeth (SHEDs) via comprehensive proteomics analysis, corresponding (H-Exo) exhibited superior in promoting cellular...
The direct growth of uniform graphene disks and their continuous film is achieved by exploiting the molten state glass. use glass enables highly nucleation an enhanced rate (tenfold) graphene, as compared to those scenarios on commonly used insulating solids. obtained glasses show promising application potentials in daily-life such smart heating devices biocompatible cell-culture mediums. As a service our authors readers, this journal provides supporting information supplied authors. Such...
Abstract B lymphocytes are activated upon Ag sensing by BCRs. The substrate presenting the can show different degrees of stiffness. It is not clear whether cells respond to changes in In this study we use high-resolution, high-speed live cell imaging techniques capture molecular events activation after recognition Ags tethered polyacrylamide gel substrates with variable stiffness as quantified Young’s modulus (2.6–22.1 kPa). We that initiation extremely sensitive exhibit much stronger...
B cells use B‐cell receptors (BCRs) to sense antigens that are usually presented on substrates with different stiffness. However, it is not known how substrate stiffness affects proliferation, class switch, and in vivo antibody responses. We addressed these questions using polydimethylsiloxane (PDMS) (20 or 1100 kPa). Live cell imaging experiments suggested stiffer more efficiently trigger the synaptic accumulation of BCR phospho‐Syk molecules compared softer substrates. In vitro expansion...
Transient receptor potential vanilloid 4 (TRPV4) is a calcium-permeable cation channel that has been associated with several types of cancer. However, its biological significance, as well related mechanism in endometrial cancer (EC) still remains elusive. In this study, we examined the function calcium EC, specific focus on TRPV4 and downstream pathway. We reported here findings high level serum ionized was significantly correlated advanced EC progression, among all channels, played an...
Atomically thin transition-metal dichalcogenides (TMDCs) have received substantial interest due to their typical thickness-dependent optical and electronic properties related applications in optoelectronics. However, the large-scale, thickness-tunable growth of such materials is still challenging. Herein, we report a fast wedding-cake-like MoS2 flakes on 6-in. soda-lime glass by using NaCl-coated Mo foils as metal precursors. The thicknesses are tuned from one layer (1L) >20L controlling...
Dental pulp necrosis are serious pathologic entities that causes tooth nutrition deficiency and abnormal root development, while regeneration of functional tissue is paramount importance to regain vitality. However, existing clinical treatments, which focus on replacing the necrotic with inactive filling materials, fail restore vitality functions, thus resulting in a devitalized weakened tooth. Currently, dental via stem cell-based therapy for pulpless teeth has raised enormous attention...
B lymphocytes use cell receptors (BCRs) to recognize membrane-bound antigens further initiate spreading and contraction responses during activation. We combined traction force microscopy live-cell imaging profile the origin, dynamics, function of generation in these responses. showed that activation required 10 20 nN when encountering presented by substrates with stiffness values from 0.5 1 kPa, which mimic rigidity antigen-presenting cells vivo. Perturbation experiments revealed F-actin...
Immune checkpoint blockades (ICBs) are a promising treatment for cancers such as melanoma by blocking important inhibitory pathways that enable tumor cells to evade immune attack. Programmed death ligand 1 monoclonal antibodies (aPDL1s) can be used an ICB significantly enhance the effectiveness of immunotherapy PD-1/PD-L1 pathway. However, aPDL1s may limited low selectivity in vivo and immunosuppressed microenvironment including hypoxia.To overcome limitations, we develop multifunctional...
Intestinal organoids, derived from intestinal stem cell self-organization, recapitulate the tissue structures and behaviors of epithelium, which hold great potential for study developmental biology, disease modeling, regenerative medicine. The epithelium is exposed to dynamic mechanical forces exert profound effects on gut development. However, conventional organoid culture system neglects key role microenvironments but relies solely biological factors. Here, we show that adding cyclic...
In recent years, although several studies have demonstrated the potential of graphene-coated substrates in promoting attachment, proliferation and differentiation osteoblasts mesenchymal stem cells (MSCs), effects single-layer graphene on osteogenic human MSCs (hMSCs) remains unclear, especially vivo. this study, we transferred chemical vapor deposition (CVD) grown to glass slides observed its adhesion, adipose-derived (hASCs) bone marrow (hBMMSCs) vitro. Then, vivo, incubated hASCs hBMMSCs...
The mechanosensing ability of lymphocytes regulates their activation in response to antigen stimulation, but the underlying mechanism remains unexplored. Here, we report that B cell mechanosensing-governed requires BCR signaling molecules. PMA-induced PKCβ can bypass Btk and PLC-γ2 molecules are usually required for cells discriminate substrate stiffness. Instead, PKCβ-dependent FAK is required, leading FAK-mediated potentiation spreading adhesion responses. inactivation or deficiency...
In recent years, dental implants have become the preferred approach for restoration of missing teeth. At present, most are made pure titanium, and affected by peri-implantitis bone resorption, which usually start from implant neck, due to complex environment in this region. To address these issues, study we modified surface titanium (Ti) exploit antibacterial osteoinductive effects single-layer graphene sheets. Chemical vapor deposition (CVD)-grown sheets were transferred discs, a method...
In this work, we reported that the quantum dot (QD) nanoparticles could be actively transported in membrane nanotubes between cardiac myocytes. Single particle imaging and tracking of QDs revealed most moved a bidirectional mode along with mean velocity 1.23 μm/s. The results suggested moving were coordinately motivated by molecular motors. It provides new information for study intercellular transportation nanoparticles.
Mechanical phenotypes of cells are found to hold vital clues reveal cellular functions and behaviors, which not only has great physiological significance but also is crucial for disease diagnosis. To this end, we developed a set electrodeformation-based biomechanical microchip assays quantify mechanical on the single-cell level. By investigating spatiotemporal dynamics cancer driven by dielectrophoresis forces, captured key global viscoelastic indexes including elasticity, viscosity,...