The global pandemic of COVID-19 cases caused by infection with SARS-CoV-2 is ongoing, no approved antiviral intervention. We describe here the effects treatment interferon-α2b in a cohort confirmed Wuhan, China. In this uncontrolled, exploratory study, 77 adults hospitalized were treated either nebulized IFN-α2b (5mU b.i.d.), arbidol (200mg t.i.d.) or combination plus arbidol. Serial testing along hematological measurements, including cell counts, blood biochemistry and serum cytokine...

We show how to apply the efficient Bayesian changepoint detection techniques of Fearnhead in multivariate setting. model joint density vector-valued observations using undirected Gaussian graphical models, whose structure we estimate. can exactly compute MAP segmentation, as well draw perfect samples from posterior over segmentations, simultaneously accounting for uncertainty about number and location changepoints, covariance structure. illustrate technique by applying it financial data bee...

Abstract Motivation: Array comparative genomic hybridization (aCGH) is a pervasive technique used to identify chromosomal aberrations in human diseases, including cancer. Aberrations are defined as regions of increased or decreased DNA copy number, relative normal sample. Accurately identifying the locations these has many important medical applications. Unfortunately, observed number changes often corrupted by various sources noise, making boundaries hard detect. One popular current uses...

Recent evidence shows that altered microRNA-9 (miR-9) expression is implicated in the progression of gastric cancer. However, exact roles and underlying mechanisms miR-9 proliferation, invasion metastasis cancer still remain unknown. In this study, was found to be down-regulated inversely correlated with cyclin D1 v-ets erythroblastosis virus E26 oncogene homolog 1 (Ets1) tissues cell lines. Bioinformatics analysis revealed putative binding sites 3′-untranslated regions (3′-UTR) Ets1 mRNA....

Although immune checkpoint blockade (ICB) therapy has shown remarkable benefits in cancers, a subset of patients with cancer exhibits unresponsiveness or develop acquired resistance due to the existence abundant immunosuppressive cells. Tumor-associated macrophages (TAMs), as dominant population, impede antitumor response; however, underlying mechanisms have not been fully elucidated yet.Single-cell RNA sequencing analysis was performed portray macrophage landscape and revealed mechanism...

Heparanase (HPSE) is the endogenous endoglycosidase that degrades heparan sulfate proteoglycans and promotes tumor growth, invasion, metastasis angiogenesis. Our previous studies have shown HPSE highly expressed in neuroblastoma (NB), most common extracranial solid childhood. However, underlying regulatory mechanisms remain largely unknown. In this study, we identified one binding site of microRNA-558 (miR-558) within promoter. NB tissues cell lines, miR-558 was up-regulated positively...

Abstract Recent evidence shows that v-ets erythroblastosis virus E26 oncogene homolog 1 (Ets1) is implicated in tumor development and progression. However, the clinical potentials underlying mechanisms of Ets1 gastric cancer progression metastasis remain largely unknown. In this study, immunostaining was identified 56 84 (66.7%) tissues, which correlated with invasion metastasis. specimens cell lines, miRNA-145 (miR-145) downregulated inversely expression. Bioinformatics analysis luciferase...

Abstract Background The global pandemic of COVID-19 cases caused by infection with SARS-CoV-2 is ongoing, no approved antiviral intervention. We describe here the effects treatment interferon-α2b in a cohort confirmed Wuhan, China. Methods In this retrospective study, 77 adults hospitalized were treated either nebulized IFN-α2b (5mU b.i.d.), arbidol (200mg t.i.d.) or combination plus arbidol. Serial testing along hematological measurements, including cell counts and blood biochemistry, serum...

The transcription factor forkhead box D3 (FOXD3) plays a crucial role in the development of neural crest cells. However, function and underlying mechanisms FOXD3 progression neuroblastoma (NB), an embryonal tumor that is derived from crest, still remain largely unknown. Here, we report important oncosuppressor NB tumorigenicity aggressiveness. We found was down-regulated tissues cell lines. Patients with high expression have greater survival probability. Over-expression or knockdown...

Recent studies have revealed the potential roles of intelectin 1 (ITLN1) in tumorigenesis. However, its functions and underlying mechanisms neuroblastoma (NB), most common extracranial solid tumor childhood, still remain largely unknown.Human cell lines were treated with recombinant ITLN1 protein or stably transfected expression short hairpin RNA vectors. Gene signaling pathway detected by western blot real-time quantitative RT-PCR. promoter activity transcription factor binding luciferase...

Matrix metalloproteinase 14 (MMP-14), a membrane-anchored MMP that promotes the tumorigenesis and aggressiveness, is highly expressed in gastric cancer. However, transcriptional regulators of MMP-14 expression cancer still remain largely unknown. In this study, through mining computational algorithm programs chromatin immunoprecipitation datasets, we identified adjacent binding sites myeloid zinc finger 1 (MZF1) miRNA-337-3p (miR-337-3p) within promoter. We demonstrated MZF1 directly bound...

Malignant pleural effusion (MPE) is a functional 'cold' tumor microenvironment in which the antitumor activity of CD8+ T cells and natural killer (NKT)-like suppressed function regulatory (Treg) enhanced. Using flow cytometry immunofluorescence staining, we detected distinct subset NKT-like expressing FOXP3 MPE. Through single-cell RNA sequencing (scRNA-seq) analysis, found that glycolysis pathway pyruvate metabolism were highly activated FOXP3+ cells. Similar to Treg cells, expressed...

Abstract Background Recent evidence indicates that methyl jasmonate (MJ), a plant stress hormone, exhibits anti-cancer activity on human cancer cells. The aim of this study is to determine whether sub-cytotoxic MJ can abolish the migration, invasion and angiogenesis gastric Methods Human cell lines SGC-7901 MKN-45 were treated with diverse concentrations MJ. Cell viability, proliferation, capabilities cells measured by MTT colorimetry, EdU incorporation, scratch assay, matrigel tube...

// Dan Li 1, * , Xiang Zhao Yong Xiao Hong Mei 1 Jiarui Pu Xuan Wanju Jiao 2 Huajie Song Hongxia Qu Kai Huang 3, 4 Liduan Zheng 2, 3 Qiangsong Tong Department of Surgery, Union Hospital, Tongji Medical College, Huazhong University Science and Technology, Wuhan, P. R. China Pathology, Clinical Center Human Genomic Research, Cardiology, These authors have contributed equally to this work Correspondence to: Tong, e-mail: qs_tong@hotmail.com Zheng, ld_zheng@hotmail.com Keywords: gastric cancer,...

// Xuan Xiang 1, * , Hong Mei Zhao Jiarui Pu 1 Dan Li Hongxia Qu Wanju Jiao 2 Jihe 3 Kai Huang 4 Liduan Zheng 2, Qiangsong Tong Department of Pediatric Surgery, Union Hospital, Tongji Medical College, Huazhong University Science and Technology, Wuhan, Hubei Province, P. R. China Pathology, Burnett School Biomedical Sciences, College Medicine, Central Florida, Orlando, USA Clinical Center Human Genomic Research, These authors have contributed equally to this work Correspondence to: Tong,...

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection causes disease 2019 (COVID-19), a lung that may progress to systemic organ involvement and in some cases, death. The identification of the earliest predictors progressive would allow for therapeutic intervention those cases. In an earlier clinical study, individuals with moderate COVID-19 were treated either arbidol (ARB) or inhaled interferon (IFN)-α2b +/−ARB. IFN treatment resulted accelerated viral clearance from upper...

Tumor-infiltrating CD8+ T cells are critical for anti-tumor immunity and positively associated with patient survival. However, the mechanisms governing cell infiltration remain incompletely elucidated, particularly those involving circular RNAs (circRNAs). In this study, we characterized circRNA expression profiles in four paired normal tumor tissues of non-small-cell lung cancer (NSCLC) identified that circFNDC3B, a transcript derived from exons 2 3 fibronectin type III domain containing 3B...

// Jieyu Zhou 1, * Maocai Li Wenbin Yu 2, Wenming 1 , Juan Wang Xuan Xiang Guojun 3 Xinliang Pan Dapeng Lei Department of Otorhinolaryngology, Qilu Hospital, Shandong University, Key Laboratory Otolaryngology, Ministry Health, Shandong, P. R. China 2 Carcinogenesis and Translational Research (Ministry Education/Beijing), Head Neck surgery, Peking University Cancer Hospital & Institute, Beijing, Surgery, The Texas MD Anderson Center, Houston, TX, USA These authors contributed equally to this...

Background: Regulatory T cells (Tregs) may represent a major cellular mechanism in immune suppression by dampening the anti-tumor response malignant pleural effusion (MPE). Tumor necrosis factor receptor type II (TNFR2) has emerged as novel identification for maximally suppressive subset of Tregs tumor environment. At present, significance TNFR2 expression on MPE remains unclear. Methods: The distribution TNFR2+cells and effector (Teffs) MPE, peripheral blood (PB), tuberculosis (TPE) were...