A5024376271- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Microbial Metabolites in Food Biotechnology
- Redox biology and oxidative stress
- Lysosomal Storage Disorders Research
- Photosynthetic Processes and Mechanisms
- Carbohydrate Chemistry and Synthesis
- Enzyme Catalysis and Immobilization
- Biochemical effects in animals
- Steroid Chemistry and Biochemistry
- Microbial Metabolic Engineering and Bioproduction
- Glycosylation and Glycoproteins Research
- Enzyme Structure and Function
- Photoreceptor and optogenetics research
- Bioactive Natural Diterpenoids Research
- Trypanosoma species research and implications
- Phytochemistry and Bioactivity Studies
- Adipose Tissue and Metabolism
- Biopolymer Synthesis and Applications
- Glycogen Storage Diseases and Myoclonus
- Bacterial Genetics and Biotechnology
- Viral Infectious Diseases and Gene Expression in Insects
- Galectins and Cancer Biology
- Metal-Catalyzed Oxygenation Mechanisms
- Chemical Looping and Thermochemical Processes
Suranaree University of Technology
2014-2025
Synchrotron Light Research Institute
2019-2025
Osaka University
2016-2019
Protein Research Foundation
2016-2018
Chulabhorn Research Institute
2012-2017
Chulabhorn Graduate Institute
2012
Hemolysin co-regulated protein 1 (Hcp1) is a component of the cluster Type VI secretion system (T6SS1) that plays key role during intracellular lifecycle Burkholderia pseudomallei . Hcp1 recognized as promising target antigen for developing melioidosis diagnostics and vaccines. While gene encoding retained across B strains, variants hcp1 have recently been identified. This study aimed to examine prevalence in clinical isolates , assess antigenicity variants, ability strains expressing these...
O-GlcNAcylation is a dynamic PTM of nuclear and cytoplasmic proteins, regulated by O-GlcNAc transferase (OGT) O-GlcNAcase, which catalyze the addition removal O-GlcNAc, respectively. This modification associated with glucose metabolism, plays important roles in many diseases including cancer. Although emerging evidence reveals that some tumor-associated proteins are modified, total cancer still largely unexplored. Here, we demonstrate was increased primary breast malignant tumors, not benign...
Abstract Calcium (Ca 2+ ) and redox signalling play important roles in acclimation processes from archaea to eukaryotic organisms. Herein we characterized a unique protein Chlamydomonas reinhardtii that has the competence integrate Ca - redox-related signalling. This protein, designated as calredoxin (CRX), combines four -binding EF-hands thioredoxin (TRX) domain. A crystal structure of CRX, at 1.6 Å resolution, revealed an unusual calmodulin-fold EF-hands, which is functionally linked via...
Human glucosylcerebrosidase 2 (GBA2) of the CAZy family GH116 is responsible for breakdown glycosphingolipids on cytoplasmic face endoplasmic reticulum and Golgi apparatus. Genetic defects in GBA2 result spastic paraplegia cerebellar ataxia, while cross-talk between GBA1 glucosylceramidases may affect Gaucher disease. Here, we report first three-dimensional structure any enzyme, Thermoanaerobacterium xylanolyticum TxGH116 β-glucosidase, alone complex with diverse ligands. These structures...
Abstract Background Pompe disease is a lysosomal storage disorder caused by the deficiency of acid alpha-glucosidase (EC. 3.2.1.20) due to mutations in human GAA gene. The objective present study was examine clinical and molecular characteristics infantile-onset (IOPD) Thailand. Methods Twelve patients with including 10 Thai two other Asian ethnicities were enrolled. To patients, gene analyzed PCR amplification direct Sanger-sequencing 20 exons coding region. novel transiently transfected...
Glycoside hydrolases (GH) bind tightly to the sugar moiety at glycosidic bond being hydrolyzed stabilize its transition state conformation. We endeavored assess importance of glucose-binding residues in GH family 116 (GH116) β-glucosidases, which include human β-glucosylceramidase 2 (GBA2), by mutagenesis followed kinetic characterization, X-ray crystallography, and ONIOM calculations on Thermoanaerobacterium xylanolyticum TxGH116, structural model for GH116 enzymes. Mutations that glucose...
Peroxiredoxins (PRXs) are a group of antioxidant enzymes that found in all organisms, including plants and green algae. The 2-Cys PRX from Chlamydomonas reinhardtii (CrPRX1) is chloroplast-localized protein critical for clearing reactive oxygen species chloroplasts. CrPRX1 reduced by thioredoxins or calredoxin (CrCRX), recently identified calcium-dependent redox protein. molecular interaction between PRXs thioredoxin/CrCRX functionally important, but discussion has been limited owing to lack...
Calcium (Ca
The Thermoanaerobacterium xylanolyticum gene product TxGH116, a glycoside hydrolase family 116 protein of 806 amino-acid residues sharing 37% sequence identity over 783 with human glucosylceramidase 2 (GBA2), was expressed in Escherichia coli . Purification by heating, immobilized metal-affinity and size-exclusion chromatography produced >90% pure TxGH116 an apparent molecular mass 90 kDa on SDS–PAGE. purified enzyme hydrolyzed the p -nitrophenyl ( NP) glycosides NP-β-D-glucoside,...
Mucopolysaccharidosis type I (MPS I) is a rare autosomal-recessive disorder caused by defects in alpha-L-iduronidase (IDUA), lysosomal enzyme encoded the IDUA gene. Herein, we characterized mutations underlying mucopolysaccharidosis intermediate form (Hurler-Scheie syndrome) and its molecular pathogenic mechanisms.Clinical data, activity of leukocytes, mutation gene were analyzed. Pathogenesis associated with an was further investigated evaluating mutant cDNA sequence, protein expression...
β-Mannanase (EC 3.2.1.78) is an enzyme that cleaves within the backbone of mannan-based polysaccharides at β-1,4-linked D-mannose residues, resulting in formation mannooligosaccharides (MOS), which are potential prebiotics. The GH26 β-mannanase KMAN from Klebsiella oxytoca KUB-CW2-3 shares 49–72% amino-acid sequence similarity with β-mannanases other sources. crystal structure a resolution 2.57 Å revealed open cleft-shaped active site. based on (β/α) 8 -barrel architecture, typical...
HpaR is a transcription regulator in the MarR family that controls expression of gene cluster responsible for conversion p ‐hydroxyphenylacetate to pyruvate and succinate cellular metabolism. Here, we report biochemical structural characterization Acinetobacter baumannii ( Ab HpaR) its complex with cognate DNA. Our study revealed binds upstream divergently transcribed hpaA meta ‐cleavage operon, as well hpaR gene, thereby repressing their by blocking access RNA polymerase. Structural...
Abstract The investigation of a plant glycosylated serine protease (EuRP‐61) isolated from Euphorbia resinifera latex for potential antiplatelet and anticoagulation activities has been previously reported. In the present study, protein sequence native crystal structure EuRP‐61 were characterized. was identified using single‐wavelength anomalous diffraction with refinement resolution 1.7 Å (PDB ID: 7EOX). main structural components composed three domains: catalytic, protease‐associated (PA),...
We designed and synthesized a fatty aldehyde surrogate containing formyl thioester group, which can be reduced by reductase (FALR) with stoichiometric formaldehyde generation. It rapidly visualized quantified using the Purpald assay. demonstrated its successful application in high throughput screening of FALR engineering.